FF-10501
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 206706

CAS#: 56973-26-3 (free base)

Description: FF-1050 is an IMPDH Inhibitor. FF-10501 Possesses Activating Effects on Myeloid Differentiation As Well As Anti-Proliferation. FF-10501 inhibited the proliferation of 9 cell-lines of hematological malignancy, including K562, HL-60 and MOLM-13. FF-10501 could ameliorate myelosuppression during MDS treatment by maintaining myelopoiesis, and the low-dose therapy for low-risk MDS patients might be effective for the improving refractory anemia.

Chemical Structure

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FF-10501
CAS# 56973-26-3 (free base)
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 206706
Name: FF-10501
CAS#: 56973-26-3 (free base)
Chemical Formula: C4H5N3O2
Exact Mass: 127.04
Molecular Weight: 127.103
Elemental Analysis: C, 37.80; H, 3.97; N, 33.06; O, 25.17

Price and Availability

Size Price Availability Quantity
25mg USD 90 Ready to ship
50mg USD 150 Ready to ship
100mg USD 225 2 weeks
200mg USD 350 2 weeks
500mg USD 650 2 Weeks
1g USD 950 2 Weeks
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Related CAS #: 56973-26-3 (free base)   66934-73-4 (HCl)   1131657-91-4 (tosylate)   1131657-83-4 (besylate)   1131657-85-6 (mesylate)   1428902-56-0 (hydrate)   66934-72-3 (sodium)   86011-08-7 (HBr)   1428902-57-1 (HCl hydrate)    

Synonym: FF-10501; FF 10501; FF10501.

IUPAC/Chemical Name: un5-hydroxy-1H-imidazole-4-carboxamide

InChi Key: UEWSIIBPZOBMBL-UHFFFAOYSA-N

InChi Code: InChI=1S/C4H5N3O2/c5-3(8)2-4(9)7-1-6-2/h1,9H,(H2,5,8)(H,6,7)

SMILES Code: O=C(N)C1=C(O)NC=N1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, not in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info: Structure was from Cancers 2019, 11, 1346

Product Data:
Product Data
Certificate of Analysis:
View CoA: current batch, Lot#A20B03B12
Safety Data Sheet (SDS):
View Safety Data Sheet (SDS)
Instruction:
View handling instruction
Biological target: FF-1050 is an IMPDH Inhibitor.
In vitro activity: An in vitro study was conducted to elucidate the mechanisms of FF-10501-induced cell death using 12 hematological malignant cell lines derived from myeloid and lymphoid malignancies. FF-10501 suppressed the growth of each cell line in a dose-dependent manner. However, the clinically relevant dose (40 μM) of FF-10501 induced cell death in three cell lines (MOLM-13, OCI-AML3, and MOLT-3). Investigation of the cell death mechanism suggested that FF-10501 induces both apoptotic and necrotic cell death. FF-10501-induced apoptosis was mediated by caspase-8 activation followed by activation of the mitochondrial pathway in MOLM-13 and MOLT-3 cells. FF-10501 induced necrotic cell death via endoplasmic reticulum stress in OCI-AML3 cells. Reference: Int J Hematol. 2019 Nov;110(5):606-617. https://dx.doi.org/10.1007/s12185-019-02722-x
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 127.10 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol: 1. Matsumoto T, Jimi S, Migita K, Terada K, Mori M, Takamatsu Y, Suzumiya J, Hara S. FF-10501 induces caspase-8-mediated apoptotic and endoplasmic reticulum stress-mediated necrotic cell death in hematological malignant cells. Int J Hematol. 2019 Nov;110(5):606-617. doi: 10.1007/s12185-019-02722-x. Epub 2019 Aug 12. PMID: 31407254.
In vivo protocol:

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1: Matsumoto T, Jimi S, Migita K, Terada K, Mori M, Takamatsu Y, Suzumiya J, Hara
S. FF-10501 induces caspase-8-mediated apoptotic and endoplasmic reticulum
stress-mediated necrotic cell death in hematological malignant cells. Int J
Hematol. 2019 Nov;110(5):606-617. doi: 10.1007/s12185-019-02722-x. Epub 2019 Aug
12. PubMed PMID: 31407254.


2: Ichii M, Oritani K, Murase M, Komatsu K, Yamazaki M, Kyoden R, Kito N, Nozaki
Y, Saito M, Iwamura H, Kanakura Y. Molecular targeting of
inosine-5'-monophosphate dehydrogenase by FF-10501 promotes erythropoiesis via
ROS/MAPK pathway. Leuk Lymphoma. 2018 Feb;59(2):448-459. doi:
10.1080/10428194.2017.1339878. Epub 2017 Jul 21. PubMed PMID: 28730859.


3: Murase M, Iwamura H, Komatsu K, Saito M, Maekawa T, Nakamura T, Yokokawa T,
Shimada Y. Lack of cross-resistance to FF-10501, an inhibitor of
inosine-5'-monophosphate dehydrogenase, in azacitidine-resistant cell lines
selected from SKM-1 and MOLM-13 leukemia cell lines. Pharmacol Res Perspect. 2016
Jan 28;4(1):e00206. doi: 10.1002/prp2.206. eCollection 2016 Feb. PubMed PMID:
26977297; PubMed Central PMCID: PMC4777262.