Ambamustine

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 592768

CAS#: 85754-59-2 (free base)

Description: Ambamustine, also known as PTT-119, is a novel tripeptide anticancer agent. PTT 119 was demonstrated to have antileukemic activity on murine and human cell lines. Most leukemic cell populations appeared to be responsive to the drug in a dose-related fashion. By cluster analysis, it was possible to discriminate subsets of samples according to PTT 119 sensitivity, and to investigate cross resistance with cytosine arabinoside and daunorubicin. PTT 119 may deserve applications in the treatment of acute nonlymphoblastic leukemia patients.

Chemical Structure

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Ambamustine
CAS# 85754-59-2 (free base)
Instruction

Theoretical Analysis

MedKoo Cat#: 592768
Name: Ambamustine
CAS#: 85754-59-2 (free base)
Chemical Formula: C29H39Cl2FN4O4S
Exact Mass: 628.21
Molecular Weight: 629.610
Elemental Analysis: C, 55.32; H, 6.24; Cl, 11.26; F, 3.02; N, 8.90; O, 10.16; S, 5.09

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Related CAS #: 85754-59-2 (free base)   83996-50-3 (HCl)    

Synonym: Ambamustine; PT 119; PT-119; PT119; PTT119; PTT-119; PTT 119;

IUPAC/Chemical Name: N-(3-(m-(Bis(2-chloroethyl)amino)phenyl)-N-(3-(p-fluorophenyl)-L-alanyl)-L-alanyl)-L-methionine, ethyl ester

InChi Key: XPGDODOEEWLHOI-GSDHBNRESA-N

InChi Code: InChI=1S/C29H39Cl2FN4O4S/c1-3-40-29(39)25(11-16-41-2)34-28(38)26(35-27(37)24(33)18-20-7-9-22(32)10-8-20)19-21-5-4-6-23(17-21)36(14-12-30)15-13-31/h4-10,17,24-26H,3,11-16,18-19,33H2,1-2H3,(H,34,38)(H,35,37)/t24-,25-,26-/m0/s1

SMILES Code: CSCC[C@@H](C(OCC)=O)NC([C@H](CC1=CC=CC(N(CCCl)CCCl)=C1)NC([C@H](CC2=CC=C(F)C=C2)N)=O)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 629.61 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Ardizzoni A, Antonelli G, Ricci S, Frasci G, Rinaldi M, Boni L, Galletti P, Pennucci MC, Antonuzzo A, Gravina A, Galli L, Comella G, Conte PF, Salvati F, Rosso R. Ambamustine in the second-line treatment of patients with small-cell lung cancer: a phase II Fonicap study. Am J Clin Oncol. 2000 Feb;23(1):22-5. PubMed PMID: 10683068.

2: Manna A, Porcellini A, Visani G, Marchetti-Rossi MT, Tura S. Limiting dilution analysis of a novel tripeptide anticancer agent Ambamustine (PTT-119): effect on K-562, CCRF-SB and multidrug resistant LoVo-Dk cell lines. Exp Hematol. 1994 Jun;22(6):517-20. PubMed PMID: 8187848.

3: Ambamustine. PTT119. Drugs R D. 2003;4(2):119-21. PubMed PMID: 12718568.

4: Mattii L, Barale R, Petrini M. Use of the comet test in the evaluation of multidrug resistance of human cell lines. Leukemia. 1998 Apr;12(4):627-32. PubMed PMID: 9557624.

5: Yagi MJ, Bekesi JG. Differential susceptibility and cytopathic alterations induced by PTT. 119, a new cancericidal compound, p-fluoro-Phe-L-Phe-m-bis-(2-chloroethyl)amino-L-Phe-Met ethoxy HCl. Biomed Pharmacother. 1983;37(7):329-38. PubMed PMID: 6667339.

6: Coppi G, Mosconi P, Gatti T, Berti MA. Toxicological aspects of a new tripeptide mustard. Farmaco Prat. 1985 Jul;40(7):242-52. PubMed PMID: 4029370.

7: Dubini F, Sezzano P, Berti MA, Coppi G. Mutagenicity studies on PTT-119. Farmaco Prat. 1987 Oct;42(10):269-79. PubMed PMID: 3322876.

8: Coppi G, Berti MA, Gatti T, Mosconi P. Some pharmacological studies on a new tripeptide mustard. Farmaco Sci. 1984 Jan;39(1):26-34. PubMed PMID: 6705911.

9: Visani G, Lemoli RM, Tosi P, Verlicchi F, Gamberi B, Cenacchi AR, Colombini R, Fogli M, Russo D, Zuffa E, et al. Sensitivity of chronic myeloid leukemia hemopoietic progenitors to PTT-119 in combination with human recombinant interferon alpha and gamma. Blut. 1990 May;60(5):287-90. PubMed PMID: 2112414.

10: Pannuti F, Coppi G, Borella F, Martoni A, Urbano L, Melotti B. Pharmacokinetics of PTT-119 in man. Chemioterapia. 1988 Apr;7(2):113-6. PubMed PMID: 3396115.

11: Tura S, Mazza P, Gherlinzoni F, Zinzani PL, Poletti G, Visani G, Lemoli RM, Bandini G, Cavo M, Galieni P, et al. Phase II study with a new alkylating agent (PTT-119) in lymphoid malignancies. Haematologica. 1988 Nov-Dec;73(6):503-8. PubMed PMID: 3148513.

12: Lemoli RM, Visani G, Tosi P, Mazza P, Motta MR, Rizzi S, Zinzani PL, Poluzzi C, Gherlinzoni F, Tura S. Effects of a new bifunctional alkylating agent (PTT-119) on in vitro growth of human cell lines and normal myeloid progenitors (CFU-GM). Haematologica. 1988 May-Jun;73(3):195-200. PubMed PMID: 3139515.

13: De Barbieri A, Dall'Asta L, Comini A, Springolo V, Mosconi P, Coppi G. Synthesis, acute toxicity and chemotherapeutic anti-cancer activities of a new tripeptidic mustard. Farmaco Sci. 1983 Apr;38(4):205-18. PubMed PMID: 6861998.

14: Bianchi R, Nardelli B, Allegrucci M, Fioretti MC. [Therapeutic effectiveness of PTT-119 evaluated in vivo in experimental models]. G Ital Chemioter. 1985 Jan-Apr;32(1):1-6. Italian. PubMed PMID: 3830770.

15: Roboz J, Greaves J, Yagi MJ, Holland JF, Bekesi JG. Analytical and pharmacological studies on a new antineoplastic tripeptide, PTT.119. Pharmacology. 1985;30(1):52-9. PubMed PMID: 3975256.

16: Dal Pozzo O, Santini V, Saccardi R, Bernabei PA, Rossi Ferrini P. Assessment of PTT 119 activity on acute nonlymphoblastic leukemia cells by [3H]-thymidine uptake inhibition and cluster analysis. Haematologica. 1990 Jan-Feb;75(1):50-3. PubMed PMID: 2338287.

17: Verlicchi F, Boschi S, Visani G, Guidi A, Tosi P, Cavo M, Tura S. Evidence for increased intracellular transport of m-sarcolysine (alkylating moiety) when combined with two amino acid analogs (PTT-119). Blut. 1990 Nov;61(5):311-3. PubMed PMID: 2271778.

18: Tura S, Mazza P, Gherlinzoni F, Zinzani PL, Poletti G, Visani G, Lemoli RM, Cavo M, Galieni P, Tassi C, et al. Phase II study of a new alkylating agent (PTT-119) in resistant-relapsed non-Hodgkin's lymphomas. Cancer Chemother Pharmacol. 1989;23(2):123-5. PubMed PMID: 2910510.

19: Yagi MJ, Zanjani M, Holland JF, Bekesi JG. Increased cancericidal activity of PTT.119; a new synthetic bis-(2-chloroethyl)amino-L-phenylalanine derivative with carrier amino acids. II. In vivo bioassay. Cancer Chemother Pharmacol. 1984;12(2):77-82. PubMed PMID: 6697428.

20: Allegrucci M, Ballerini P, Romani L, Frati L. Immunopharmacological studies of PTT.119, a new synthetic bis-(2-chloroethyl)amino-L-phenylalanine derivative. Immunopharmacol Immunotoxicol. 1987;9(1):71-86. PubMed PMID: 3450712.