Resazurin sodium | CAS#62758-13-8 | cell viability indicator

Resazurin sodium
featured

WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 562949

CAS#: 62758-13-8 (sodium)

Description: Resazurin is an oxidation-reduction indicator in cell viability. Resazurin is a blue, non-fluorescent dye originally used to test milk/biological fluids for bacterial and yeast contamination. It is also useful in sperm viability and semen quality test. Resazurin is applicable in cytotoxicity determination. Resazurin sodium salt has been used in viability assay: such as to measure cell viability of A. fumigatus; to assess cell proliferation; and to determine A. fumigatus viability in high-throughput screenings of microbial natural extracts.

Chemical Structure

Resazurin sodium

CAS# 62758-13-8 (sodium)

Instruction

Theoretical Analysis

MedKoo Cat#: 562949
Name: Resazurin sodium
CAS#: 62758-13-8 (sodium)
Chemical Formula: C12H6NNaO4
Exact Mass: 229.04
Molecular Weight: 251.173
Elemental Analysis: C, 57.38; H, 2.41; N, 5.58; Na, 9.15; O, 25.48

Price and Availability

Size	Price	Availability
1g	USD 250	2 Weeks
5g	USD 550	2 Weeks
10g	USD 750	2 Weeks
25g	USD 1250	2 Weeks
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Related CAS #: 62758-13-8 (sodium) 550-82-3 (free)

Synonym: Resazurin sodium; Resazurin sodium salt; Resazurin; Diazoresorcinol; Azoresorcin; Resazoin; Resazurine;

IUPAC/Chemical Name: 7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt

InChi Key: IVGPGQSSDLDOLH-UHFFFAOYSA-M

InChi Code: InChI=1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1

SMILES Code: O=C1C=CC2=[N+]([O-])C3=C(C=C([O-])C=C3)OC2=C1.[Na+]

Appearance: Solid powder

Purity: Dye content, ~90%

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Instruction:

View handling instruction

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 251.17 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:
In vitro protocol:
In vivo protocol:

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt* g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1

Volume 1

Concentration 2

Volume 2

1: Lin S, Yang L, Chen G, Li B, Chen D, Li L, Xu Z. Pathogenic features and characteristics of food borne pathogens biofilm: Biomass, viability and matrix. Microb Pathog. 2017 Oct;111:285-291. doi: 10.1016/j.micpath.2017.08.005. Epub 2017 Aug 9. Review. PubMed PMID: 28803003.

2: Riss TL, Moravec RA, Niles AL, Duellman S, Benink HA, Worzella TJ, Minor L. Cell Viability Assays. 2013 May 1 [updated 2016 Jul 1]. In: Sittampalam GS, Coussens NP, Brimacombe K, Grossman A, Arkin M, Auld D, Austin C, Baell J, Bejcek B, Chung TDY, Dahlin JL, Devanaryan V, Foley TL, Glicksman M, Hall MD, Hass JV, Inglese J, Iversen PW, Kahl SD, Kales SC, Lal-Nag M, Li Z, McGee J, McManus O, Riss T, Trask OJ Jr., Weidner JR, Xia M, Xu X, editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Available from http://www.ncbi.nlm.nih.gov/books/NBK144065/ PubMed PMID: 23805433.

3: Hall MD, Simeonov A, Davis MI. Avoiding Fluorescence Assay Interference-The Case for Diaphorase. Assay Drug Dev Technol. 2016 Apr;14(3):175-9. doi: 10.1089/adt.2016.707. Review. PubMed PMID: 27078679; PubMed Central PMCID: PMC4840916.

4: Coban AY, Deveci A, Sunter AT, Palomino JC, Martin A. Resazurin microtiter assay for isoniazid, rifampicin, ethambutol and streptomycin resistance detection in Mycobacterium tuberculosis: Updated meta-analysis. Int J Mycobacteriol. 2014 Dec;3(4):230-41. doi: 10.1016/j.ijmyco.2014.09.002. Epub 2014 Sep 29. Review. PubMed PMID: 26786621.

5: Alnimr AM. Dormancy models for Mycobacterium tuberculosis: A minireview. Braz J Microbiol. 2015 Jul 1;46(3):641-7. doi: 10.1590/S1517-838246320140507. eCollection 2015 Jul-Sep. Review. PubMed PMID: 26413043; PubMed Central PMCID: PMC4568887.

6: Pantanella F, Valenti P, Natalizi T, Passeri D, Berlutti F. Analytical techniques to study microbial biofilm on abiotic surfaces: pros and cons of the main techniques currently in use. Ann Ig. 2013 Jan-Feb;25(1):31-42. doi: 10.7416/ai.2013.1904. Review. PubMed PMID: 23435778.

7: Rampersad SN. Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays. Sensors (Basel). 2012;12(9):12347-60. doi: 10.3390/s120912347. Epub 2012 Sep 10. Review. PubMed PMID: 23112716; PubMed Central PMCID: PMC3478843.

8: Vega-Avila E, Pugsley MK. An overview of colorimetric assay methods used to assess survival or proliferation of mammalian cells. Proc West Pharmacol Soc. 2011;54:10-4. Review. PubMed PMID: 22423572.

9: Matteucci E, Giampietro O. Flow cytometry study of leukocyte function: analytical comparison of methods and their applicability to clinical research. Curr Med Chem. 2008;15(6):596-603. Review. PubMed PMID: 18336274.

10: Comhaire F, Vermeulen L. Human semen analysis. Hum Reprod Update. 1995 Jul;1(4):343-62. Review. PubMed PMID: 9080212.

Your view history

Resazurin sodium featured