Sulfo-LC-SPDP Crosslinker
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MedKoo CAT#: 571613

CAS#: 169751-10-4 (sodium)

Description: Sulfo-LC-SPDP Crosslinker, or Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate, is a cleavable, water soluble, amino and thiol (sulfhydryl) reactive heterobifunctional protein crosslinker. The "long chain" Sulfo-LC-SPDP crosslinking reagent has a 15.7 angstrom spacer arm, and is the water soluble analog of LC-SPDP crosslinker.

Chemical Structure

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Sulfo-LC-SPDP Crosslinker
CAS# 169751-10-4 (sodium)
Instruction

Theoretical Analysis

MedKoo Cat#: 571613
Name: Sulfo-LC-SPDP Crosslinker
CAS#: 169751-10-4 (sodium)
Chemical Formula: C18H22N3NaO8S3
Exact Mass: 0.00
Molecular Weight: 527.560
Elemental Analysis: C, 40.98; H, 4.20; N, 7.97; Na, 4.36; O, 24.26; S, 18.23

Price and Availability

Size Price Availability Quantity
100mg USD 750 2 Weeks
1g USD 4850 2 Weeks
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Related CAS #: 169751-10-4 (sodium)   150244-18-1 (acid)    

Synonym: Sulfo-LC-SPDP Crosslinker, Sulfo-LC-SPDP Cross linker, Sulfo-LC-SPDP Cross-linker; Sulfo-AC5-SPDP; Sulfo-LC-SPDP;

IUPAC/Chemical Name: 3-Pyrrolidinesulfonic acid, 2,5-dioxo-1-[[1-oxo-6-[[1-oxo-3-(2-pyridinyldithio)propyl]amino]hexyl]oxy]-, monosodium salt

InChi Key: ZAPNXDUFCQIHFS-UHFFFAOYSA-M

InChi Code: InChI=1S/C18H23N3O8S3.Na/c22-14(8-11-30-31-15-6-3-5-10-20-15)19-9-4-1-2-7-17(24)29-21-16(23)12-13(18(21)25)32(26,27)28;/h3,5-6,10,13H,1-2,4,7-9,11-12H2,(H,19,22)(H,26,27,28);/q;+1/p-1

SMILES Code: O=S(C(C1)C(N(OC(CCCCCNC(CCSSC2=NC=CC=C2)=O)=O)C1=O)=O)([O-])=O.[Na+]

Appearance: Solid powder

Purity: >95% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info: Sulfo-LC-SPDP crosslinker's reactive groups include N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains; while on the other end of the Sulfo-LC-SPDP crosslinking molecule pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond. The disulfide bond can be reduced with TCEP-HCl or DTT to reverse the crosslinked proteins.

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 527.56 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Weston LA, Bauer KM, Skube SB, Hummon AB. Selective, bead-based global peptide capture using a bifunctional cross-linker. Anal Chem. 2013 Nov 19;85(22):10675-9. doi: 10.1021/ac401825m. Epub 2013 Oct 28. PubMed PMID: 24117407; PubMed Central PMCID: PMC4118834.