FM04

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MedKoo CAT#: 130543

CAS#: FM04

Description: FM04 is a novel potent P-glycoprotein (P-gp) inhibitor (EC50 = 83 nM). It interacts with Q1193 and I1115 in the nucleotide-binding domain 2 (NBD2) of human P-gp, which disrupts the R262-Q1081-Q1118 interaction pocket and uncoupling ICL2-NBD2 interaction, thus inhibiting P-gp.

Chemical Structure

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FM04
CAS# FM04
Instruction

Theoretical Analysis

MedKoo Cat#: 130543
Name: FM04
CAS#: FM04
Chemical Formula: C26H25NO4
Exact Mass: 415.18
Molecular Weight: 415.490
Elemental Analysis: C, 75.16; H, 6.07; N, 3.37; O, 15.40

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: FM-04, FM 04

IUPAC/Chemical Name: 2-(4-(2-(2-(Benzylamino)ethoxy)ethoxy)phenyl)-4H-chromen-4-one

InChi Key: JIFJFNBTQSCGBM-UHFFFAOYSA-N

InChi Code: InChI=1S/C26H25NO4/c28-24-18-26(31-25-9-5-4-8-23(24)25)21-10-12-22(13-11-21)30-17-16-29-15-14-27-19-20-6-2-1-3-7-20/h1-13,18,27H,14-17,19H2

SMILES Code: O=C1C=C(C2=CC=C(OCCOCCNCC3=CC=CC=C3)C=C2)OC4=C1C=CC=C4

Appearance: To be determined

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 415.49 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1. Liu Z, Wong ILK, Sang J, Liu F, Yan CSW, Kan JWY, Chan TH, Chow LMC. Identification of Binding Sites in the Nucleotide-Binding Domain of P-Glycoprotein for a Potent and Nontoxic Modulator, the Amine-Containing Monomeric Flavonoid FM04. J Med Chem. 2023 May 11;66(9):6160-6183. doi: 10.1021/acs.jmedchem.2c02005. Epub 2023 Apr 25. PMID: 37098275.

2. Kan JWY, Yan CSW, Wong ILK, Su X, Liu Z, Chan TH, Chow LMC. Discovery of a Flavonoid FM04 as a Potent Inhibitor to Reverse P-Glycoprotein-Mediated Drug Resistance in Xenografts and Improve Oral Bioavailability of Paclitaxel. Int J Mol Sci. 2022 Dec 4;23(23):15299. doi: 10.3390/ijms232315299. PMID: 36499627; PMCID: PMC9739180.