TM-25659

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 130223

CAS#: 260553-97-7

Description: TM-25659 is a TAZ agonist that plays an important role in cell proliferation and differentiation. TM-25659 has been shown to enhance osteogenic differentiation and interact with peroxisome proliferator-activated receptor gamma, inhibiting differentiation of adipocytes for the reduction of insulin resistance.

Chemical Structure

img
TM-25659
CAS# 260553-97-7
Instruction

Theoretical Analysis

MedKoo Cat#: 130223
Name: TM-25659
CAS#: 260553-97-7
Chemical Formula: C30H28N8
Exact Mass: 500.24
Molecular Weight: 500.610
Elemental Analysis: C, 71.98; H, 5.64; N, 22.38

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

Request quote for custom synthesis

Synonym: TM-25659; TM25659; TM 25659

IUPAC/Chemical Name: 3-((2'-(2H-tetrazol-5-yl)-[1,1'-biphenyl]-4-yl)methyl)-2-butyl-5-methyl-6-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridine

InChi Key: KCOQNLYGMQJUJD-UHFFFAOYSA-N

InChi Code: InChI=1S/C30H28N8/c1-3-4-11-28-33-27-17-26(23-8-7-16-31-18-23)20(2)32-30(27)38(28)19-21-12-14-22(15-13-21)24-9-5-6-10-25(24)29-34-36-37-35-29/h5-10,12-18H,3-4,11,19H2,1-2H3,(H,34,35,36,37)

SMILES Code: CC1=C(C2=CC=CN=C2)C=C3C(N(CC4=CC=C(C5=CC=CC=C5C6=NNN=N6)C=C4)C(CCCC)=N3)=N1

Appearance: To be determined

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 500.61 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1. Huang H, Lu Q, Ye C, Wei M, Yang C, Zhang L, Huang Y, Luo X, Luo J. TAZ promotes osteogenic differentiation of mesenchymal stem cells line C3H10T1/2, murine multi-lineage cells lines C2C12, and MEFs induced by BMP9. Cell Death Discov. 2022 Dec 27;8(1):499. doi: 10.1038/s41420-022-01292-y. PMID: 36575168; PMCID: PMC9794779.

2. Kodaka M, Mao F, Arimoto-Matsuzaki K, Kitamura M, Xu X, Yang Z, Nakagawa K, Maruyama J, Ishii K, Akazawa C, Oyaizu T, Yamamoto N, Ishigami-Yuasa M, Tsuemoto N, Ito S, Kagechika H, Nishina H, Hata Y. Characterization of a novel compound that promotes myogenesis via Akt and transcriptional co-activator with PDZ-binding motif (TAZ) in mouse C2C12 cells. PLoS One. 2020 Apr 8;15(4):e0231265. doi: 10.1371/journal.pone.0231265. PMID: 32267872; PMCID: PMC7141682.

3. Zhu Y, Wu Y, Cheng J, Wang Q, Li Z, Wang Y, Wang D, Wang H, Zhang W, Ye J, Jiang H, Wang L. Pharmacological activation of TAZ enhances osteogenic differentiation and bone formation of adipose-derived stem cells. Stem Cell Res Ther. 2018 Mar 7;9(1):53. doi: 10.1186/s13287-018-0799-z. PMID: 29514703; PMCID: PMC5842656.

4. Jung JG, Yi SA, Choi SE, Kang Y, Kim TH, Jeon JY, Bae MA, Ahn JH, Jeong H, Hwang ES, Lee KW. TM-25659-Induced Activation of FGF21 Level Decreases Insulin Resistance and Inflammation in Skeletal Muscle via GCN2 Pathways. Mol Cells. 2015 Dec;38(12):1037-43. doi: 10.14348/molcells.2015.0100. Epub 2015 Nov 4. PMID: 26537193; PMCID: PMC4696994.

5. Men T, Piao SH, Teng CB. [Regulation of differentiation of mesenchymal stem cells by the Hippo pathway effectors TAZ/YAP]. Yi Chuan. 2013 Nov;35(11):1283-90. Chinese. doi: 10.3724/sp.j.1005.2013.01283. PMID: 24579311.

6. Choi MK, Kwon M, Ahn JH, Kim NJ, Bae MA, Song IS. Transport characteristics and transporter-based drug-drug interactions of TM-25659, a novel TAZ modulator. Biopharm Drug Dispos. 2014 Apr;35(3):183-94. doi: 10.1002/bdd.1883. Epub 2013 Dec 18. PMID: 24285344.

7. Lee KR, Choi SH, Song JS, Kwak EY, Chae YJ, Im SH, Lee BH, Seo H, Cho WK, Kim MS, Kim NJ, Ahn SH, Bae MA. Pharmacokinetic characterization of the novel TAZ modulator TM-25659 using a multicompartment kinetic model in rats and a possibility of its drug-drug interactions in humans. Xenobiotica. 2013 Feb;43(2):193-200. doi: 10.3109/00498254.2012.709953. Epub 2012 Aug 3. PMID: 22856387.

8. Choi SH, Lee KR, Woo JC, Kim NJ, Moon DC, Hwang ES, Ahn SH, Bae MA, Kim MS. Determination of a novel TAZ modulator, 2-butyl-5-methyl-6-(pyridine-3-yl)-3-[2'-(1H-tetrazole-5-yl)-biphenyl-4-ylmethyl]-3H imidazo[4,5-b]pyridine] (TM-25659) in rat plasma by liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal. 2012 Apr 7;63:47-52. doi: 10.1016/j.jpba.2012.01.036. Epub 2012 Feb 7. PMID: 22357285.

9. Jang EJ, Jeong H, Kang JO, Kim NJ, Kim MS, Choi SH, Yoo SE, Hong JH, Bae MA, Hwang ES. TM-25659 enhances osteogenic differentiation and suppresses adipogenic differentiation by modulating the transcriptional co-activator TAZ. Br J Pharmacol. 2012 Mar;165(5):1584-94. doi: 10.1111/j.1476-5381.2011.01664.x. PMID: 21913895; PMCID: PMC3372739.