3-IP sodium salt | CAS#3318-43-2 | Alkaline phosphatase substrate

3-IP sodium salt
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 464813

CAS#: 3318-43-2 (sodium)

Description: 3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate has been used in ELISAs.

Chemical Structure

3-IP sodium salt

CAS# 3318-43-2 (sodium)

Instruction

Theoretical Analysis

MedKoo Cat#: 464813
Name: 3-IP sodium salt
CAS#: 3318-43-2 (sodium)
Chemical Formula: C8H6NNa2O4P
Exact Mass: 0.00
Molecular Weight: 257.092
Elemental Analysis: C, 37.37; H, 2.35; N, 5.45; Na, 17.88; O, 24.89; P, 12.05

Price and Availability

Size	Price	Availability
250mg	USD 350	2 Weeks
1g	USD 950	2 Weeks
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: 3-IP sodium salt; 3IP sodium salt; 3 IP sodium salt; 3-Indoxyl Phosphate sodium salt; 3 Indoxyl Phosphate sodium salt; 3Indoxyl Phosphate sodium salt;

IUPAC/Chemical Name: sodium 1H-indol-3-yl phosphate

InChi Key: WCSKWBKPKXJWEG-UHFFFAOYSA-L

InChi Code: InChI=1S/C8H8NO4P.2Na/c10-14(11,12)13-8-5-9-7-4-2-1-3-6(7)8;;/h1-5,9H,(H2,10,11,12);;/q;2*+1/p-2

SMILES Code: O=P(OC1=CNC2=C1C=CC=C2)([O-])[O-].[Na+].[Na+]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Instruction:

View handling instruction

Biological target:
In vitro activity:
In vivo activity:

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	PBS (pH 7.2)	10.0	38.90

Preparing Stock Solutions

The following data is based on the product molecular weight 257.09 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Silva NFD, Neves MMPS, Magalhães JMCS, Freire C, Delerue-Matos C. Electrochemical immunosensor towards invasion-associated protein p60: An alternative strategy for Listeria monocytogenes screening in food. Talanta. 2020 Aug 15;216:120976. doi: 10.1016/j.talanta.2020.120976. Epub 2020 Mar 28. PMID: 32456897.

2: Wang S, Wang W, Hao C, Yunjia Y, Qin L, He M, Mao W. Antiviral activity against enterovirus 71 of sulfated rhamnan isolated from the green alga Monostroma latissimum. Carbohydr Polym. 2018 Nov 15;200:43-53. doi: 10.1016/j.carbpol.2018.07.067. Epub 2018 Jul 24. PMID: 30177184.

3: Kurland IJ, Broin PÓ, Golden A, Su G, Meng F, Liu L, Mohney R, Kulkarni S, Guha C. Integrative Metabolic Signatures for Hepatic Radiation Injury. PLoS One. 2015 Jun 5;10(6):e0124795. doi: 10.1371/journal.pone.0124795. PMID: 26046990; PMCID: PMC4457483.

4: Narayanan J, Sharma MK, Ponmariappan S, Sarita, Shaik M, Upadhyay S. Electrochemical immunosensor for botulinum neurotoxin type-E using covalently ordered graphene nanosheets modified electrodes and gold nanoparticles-enzyme conjugate. Biosens Bioelectron. 2015 Jul 15;69:249-56. doi: 10.1016/j.bios.2015.02.039. Epub 2015 Feb 26. PMID: 25754919.

5: Ge S, Yu F, Ge L, Yan M, Yu J, Chen D. Disposable electrochemical immunosensor for simultaneous assay of a panel of breast cancer tumor markers. Analyst. 2012 Oct 21;137(20):4727-33. doi: 10.1039/c2an35967g. PMID: 22937531.

6: Lai G, Yan F, Wu J, Leng C, Ju H. Ultrasensitive multiplexed immunoassay with electrochemical stripping analysis of silver nanoparticles catalytically deposited by gold nanoparticles and enzymatic reaction. Anal Chem. 2011 Apr 1;83(7):2726-32. doi: 10.1021/ac103283p. Epub 2011 Mar 3. PMID: 21370869.

7: Escamilla-Gómez V, Hernández-Santos D, González-García MB, Pingarrón-Carrazón JM, Costa-García A. Simultaneous detection of free and total prostate specific antigen on a screen-printed electrochemical dual sensor. Biosens Bioelectron. 2009 Apr 15;24(8):2678-83. doi: 10.1016/j.bios.2009.01.043. Epub 2009 Feb 6. PMID: 19261459.

8: Díaz-González M, Hernández-Santos D, González-García MB, Costa-García A. Development of an immunosensor for the determination of rabbit IgG using streptavidin modified screen-printed carbon electrodes. Talanta. 2005 Jan 30;65(2):565-73. doi: 10.1016/j.talanta.2004.07.022. PMID: 18969836.

9: Fanjul-Bolado P, González-García MB, Costa-García A. Voltammetric determination of alkaline phosphatase and horseradish peroxidase activity using 3-indoxyl phosphate as substrate Application to enzyme immunoassay. Talanta. 2004 Oct 8;64(2):452-7. doi: 10.1016/j.talanta.2004.03.003. PMID: 18969625.

10: Díaz-González M, de la Escosura-Muñiz A, González-García MB, Costa-García A. DNA hybridization biosensors using polylysine modified SPCEs. Biosens Bioelectron. 2008 Apr 15;23(9):1340-6. doi: 10.1016/j.bios.2007.12.001. Epub 2007 Dec 8. PMID: 18207382; PMCID: PMC7127112.

11: Fanjul-Bolado P, Hernández-Santos D, González-García MB, Costa-García A. Alkaline phosphatase-catalyzed silver deposition for electrochemical detection. Anal Chem. 2007 Jul 15;79(14):5272-7. doi: 10.1021/ac070624o. Epub 2007 Jun 15. PMID: 17569504.

12: Fanjul-Bolado P, González-García MB, Costa-García A. Quantitative analysis of enzymatic assays using indoxyl-based substrates. Anal Bioanal Chem. 2006 Nov;386(6):1849-54. doi: 10.1007/s00216-006-0808-4. Epub 2006 Oct 12. PMID: 17036214.

13: Kaur P, Lingner A, Singh B, Böer E, Polajeva J, Steinborn G, Bode R, Gellissen G, Satyanarayana T, Kunze G. APHO1 from the yeast Arxula adeninivorans encodes an acid phosphatase of broad substrate specificity. Antonie Van Leeuwenhoek. 2007 Jan;91(1):45-55. doi: 10.1007/s10482-006-9094-6. Epub 2006 Oct 3. PMID: 17016743.

14: Abad-Valle P, Fernández-Abedul MT, Costa-García A. DNA single-base mismatch study with an electrochemical enzymatic genosensor. Biosens Bioelectron. 2007 Mar 15;22(8):1642-50. doi: 10.1016/j.bios.2006.07.015. Epub 2006 Sep 1. PMID: 16950611; PMCID: PMC7127251.

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16: Kodaka H, Mizuochi S, Teramura H, Nirazuka T. Comparison of the compact dry YM with the FDA BAM method for enumeration of yeasts and molds in fruit-based products. Performance-Tested Method 100401. J AOAC Int. 2006 Jan- Feb;89(1):127-38. PMID: 16512237.

17: Xuan M, Fang YR, Wato M, Hata S, Tanaka A. Immunohistochemical co- localization of lymphatics and blood vessels in oral squamous cell carcinomas. J Oral Pathol Med. 2005 Jul;34(6):334-9. doi: 10.1111/j.1600-0714.2005.00316.x. PMID: 15946180.

18: Abad-Valle P, Fernández-Abedul MT, Costa-García A. Genosensor on gold films with enzymatic electrochemical detection of a SARS virus sequence. Biosens Bioelectron. 2005 May 15;20(11):2251-60. doi: 10.1016/j.bios.2004.10.019. PMID: 15797323; PMCID: PMC7126974.

19: Díaz-González M, González-García MB, Costa-García A. Immunosensor for Mycobacterium tuberculosis on screen-printed carbon electrodes. Biosens Bioelectron. 2005 Apr 15;20(10):2035-43. doi: 10.1016/j.bios.2004.09.035. PMID: 15741073.

20: Hernández-Santos D, Díaz-González M, González-García MB, Costa-García A. Enzymatic genosensor on streptavidin-modified screen-printed carbon electrodes. Anal Chem. 2004 Dec 1;76(23):6887-93. doi: 10.1021/ac048892z. PMID: 15571337.

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