DiBAC4(3) | CAS#70363-83-6 | Fluorescent membrane potential indicator

DiBAC4(3)
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 464673

CAS#: 70363-83-6

Description: DiBAC4(3) is a negatively charged fluorescent membrane potential indicator. Upon membrane depolarization, DiBAC4(3) enters the cytosol, binds to lipid membranes and intracellular proteins, and displays excitation/emission maxima of 490/516 nm, respectively. It has been used to measure the membrane potential of live or fixed mammalian and bacterial cells by flow cytometry or fluorescence microscopy.

Chemical Structure

DiBAC4(3)

CAS# 70363-83-6

Instruction

Theoretical Analysis

MedKoo Cat#: 464673
Name: DiBAC4(3)
CAS#: 70363-83-6
Chemical Formula: C27H40N4O6
Exact Mass: 516.29
Molecular Weight: 516.639
Elemental Analysis: C, 62.77; H, 7.80; N, 10.84; O, 18.58

Price and Availability

Size	Price	Availability
10mg	USD 300	2 Weeks
25mg	USD 600	2 Weeks
50mg	USD 950	2 Weeks
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: DiBAC4(3); DiBAC4 (3); DiBAC4-(3);

IUPAC/Chemical Name: (E)-1,3-dibutyl-5-(3-(1,3-dibutyl-2,4,6-trioxohexahydropyrimidin-5-yl)allylidene)pyrimidine-2,4,6(1H,3H,5H)-trione

InChi Key: ATJCYSYHWGQAET-BUHFOSPRSA-N

InChi Code: InChI=1S/C27H40N4O6/c1-5-9-16-28-22(32)20(23(33)29(26(28)36)17-10-6-2)14-13-15-21-24(34)30(18-11-7-3)27(37)31(25(21)35)19-12-8-4/h13-15,20H,5-12,16-19H2,1-4H3/b14-13+

SMILES Code: O=C1/C(C(N(C(N1CCCC)=O)CCCC)=O)=C\C=C\C2C(N(C(N(C2=O)CCCC)=O)CCCC)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Instruction:

View handling instruction

Biological target:
In vitro activity:
In vivo activity:

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMF	10.0	19.36
	DMSO	15.0	29.04
	DMSO:PBS (pH 7.2) (1:2)	0.3	0.64
	Ethanol	5.0	9.68

Preparing Stock Solutions

The following data is based on the product molecular weight 516.64 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:
In vitro protocol:
In vivo protocol:

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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1: Kim H, Lee DG. Lupeol-induced nitric oxide elicits apoptosis-like death within Escherichia coli in a DNA fragmentation-independent manner. Biochem J. 2021 Feb 26;478(4):855-869. doi: 10.1042/BCJ20200925. PMID: 33522568.

2: Nordström T, Zhenyu G, Andersson LC, Åkerman KEO. Tannic acid inhibits electrogenic Na+/HCO3- co-transporter activity in embryonic neural stem cell- derived radial glial-like cells. Neuroreport. 2020 Jan 8;31(1):57-63. doi: 10.1097/WNR.0000000000001372. PMID: 31714480; PMCID: PMC6903378.

3: Martelli A, Citi V, Calderone V. Vascular Effects of H2S-Donors: Fluorimetric Detection of H2S Generation and Ion Channel Activation in Human Aortic Smooth Muscle Cells. Methods Mol Biol. 2019;2007:79-87. doi: 10.1007/978-1-4939-9528-8_6. PMID: 31148107.

4: Wang Q, Xu Y, Dong M, Hang B, Sun Y, Wang L, Wang Y, Hu J, Zhang W. HJH-1, a Broad-Spectrum Antimicrobial Activity and Low Cytotoxicity Antimicrobial Peptide. Molecules. 2018 Aug 14;23(8):2026. doi: 10.3390/molecules23082026. PMID: 30110916; PMCID: PMC6222697.

5: Zhang P, Turnbull MW. Virus innexin expression in insect cells disrupts cell membrane potential and pH. J Gen Virol. 2018 Oct;99(10):1444-1452. doi: 10.1099/jgv.0.001132. Epub 2018 Aug 9. PMID: 30091698.

6: Murray JW, Yin D, Wolkoff AW. Reduction of organelle motility by removal of potassium and other solutes. PLoS One. 2017 Sep 18;12(9):e0184898. doi: 10.1371/journal.pone.0184898. PMID: 28922372; PMCID: PMC5602639.

7: Yun DG, Lee DG. Assessment of silibinin as a potential antifungal agent and investigation of its mechanism of action. IUBMB Life. 2017 Aug;69(8):631-637. doi: 10.1002/iub.1647. Epub 2017 Jun 21. PMID: 28636236.

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9: Guo Y, Stärk HJ, Hause G, Schmidt M, Harms H, Wick LY, Müller S. Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces. Cytometry A. 2017 Aug;91(8):775-784. doi: 10.1002/cyto.a.23055. Epub 2017 Jan 22. PMID: 28110496.

10: Pina-Vaz C, Silva AP, Faria-Ramos I, Teixeira-Santos R, Moura D, Vieira TF, Sousa SF, Costa-de-Oliveira S, Cantón R, Rodrigues AG. A Flow Cytometric and Computational Approaches to Carbapenems Affinity to the Different Types of Carbapenemases. Front Microbiol. 2016 Aug 9;7:1259. doi: 10.3389/fmicb.2016.01259. PMID: 27555844; PMCID: PMC4977277.

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