PIPES monosodium | CAS#10010-67-0 | Buffering agent

PIPES monosodium
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 464428

CAS#: 10010-67-0

Description: PIPES is frequently used as a buffering agent in biochemistry. It is useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.

Chemical Structure

PIPES monosodium

CAS# 10010-67-0

Product data Instruction

Theoretical Analysis

MedKoo Cat#: 464428
Name: PIPES monosodium
CAS#: 10010-67-0
Chemical Formula: C8H17N2NaO6S2
Exact Mass: 0.00
Molecular Weight: 324.342
Elemental Analysis: C, 29.63; H, 5.28; N, 8.64; Na, 7.09; O, 29.60; S, 19.77

Price and Availability

Size	Price	Availability
10mg	USD -2	2 Weeks
50mg	USD -2	2 Weeks
200mg	USD -2	2 Weeks
1g	USD -2	2 Weeks
2g	USD -2	2 Weeks
5g	USD -2	2 Weeks
25g	USD 220	2 Weeks
100g	USD 430	2 Weeks
500g	USD 990	2 Weeks
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: PIPES monosodium;

IUPAC/Chemical Name: sodium 2-(4-(2-sulfoethyl)piperazin-1-yl)ethane-1-sulfonate

InChi Key: OGGAIRCLBMGXCZ-UHFFFAOYSA-M

InChi Code: InChI=1S/C8H18N2O6S2.Na/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;/h1-8H2,(H,11,12,13)(H,14,15,16);/q;+1/p-1

SMILES Code: OS(=O)(CCN1CCN(CC1)CCS(=O)([O-])=O)=O.[Na+]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

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Product Data:

Product Data

Instruction:

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Biological target:	PIPES is frequently used as a buffering agent in biochemistry.
In vitro activity:	The incubation of MP (myofibrillar protein) suspensions (20 mg mL-1 in piperazine-N,N'-bis(2-ethanesulfonic acid) buffer, with 0.6 mol L-1 sodium chloride, pH 6.25) at 4 °C for 24 h under an iron-catalyzed hydroxyl radical generating system (Fenton reaction) promoted the formation of thiobarbituric acid reactive substances and protein carbonyls, which was attenuated by EC (5, 50, and 100 μmol g-1 protein). Reduced protein sulfhydryl content, tryptophan fluorescence, protein solubility, as well as increased surface hydrophobicity were found by the co-incubation of EC. Analysis by scanning electron microscopy revealed increased protein aggregation and fragments in oxidized MP, which were further enhanced by the addition of EC. However, the protein digestibility of MP was not affected. Reference: J Sci Food Agric. 2021 Jan 30;101(2):746-753. https://pubmed.ncbi.nlm.nih.gov/32706121/
In vivo activity:	This study found that piperazine-N,N'-bis(ethanesulfonic acid) (PIPES) and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), two zwitterionic buffers commonly used in tissue fixation, can cause severe lumen and cell morphological defects in Drosophila pupal and adult retina; the inter-rhabdomeral lumen becomes dilated and the photoreceptor cells are significantly reduced in size. Correspondingly, the localization pattern of Eyes shut (EYS), a luminal protein, is severely altered. In contrast, tissues fixed in the phosphate buffered saline (PBS) buffer results in lumen and cell morphologies that are consistent with live imaging. Reference: BMC Dev Biol. 2015 Feb 3;15:10. https://pubmed.ncbi.nlm.nih.gov/25645690/

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	0.1M NaOH	250.0	0.00

Preparing Stock Solutions

The following data is based on the product molecular weight 324.34 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:	1. Xu M, Sun M, Lu C, Han Y, Yao X, Niu X, Xu M, Zhu Q. Influence of epicatechin on oxidation-induced physicochemical and digestibility changes in porcine myofibrillar proteins during refrigerated storage. J Sci Food Agric. 2021 Jan 30;101(2):746-753. doi: 10.1002/jsfa.10687. Epub 2020 Aug 19. PMID: 32706121. 2. Hugel S, Kadiri N, Rodeau JL, Gaillard S, Schlichter R. pH-dependent inhibition of native GABA(A) receptors by HEPES. Br J Pharmacol. 2012 Aug;166(8):2402-16. doi: 10.1111/j.1476-5381.2012.01956.x. PMID: 22452286; PMCID: PMC3448902. 3. Nie J, Mahato S, Zelhof AC. Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation. BMC Dev Biol. 2015 Feb 3;15:10. doi: 10.1186/s12861-015-0056-y. PMID: 25645690; PMCID: PMC4320506.
In vitro protocol:	1. Xu M, Sun M, Lu C, Han Y, Yao X, Niu X, Xu M, Zhu Q. Influence of epicatechin on oxidation-induced physicochemical and digestibility changes in porcine myofibrillar proteins during refrigerated storage. J Sci Food Agric. 2021 Jan 30;101(2):746-753. doi: 10.1002/jsfa.10687. Epub 2020 Aug 19. PMID: 32706121. 2. Hugel S, Kadiri N, Rodeau JL, Gaillard S, Schlichter R. pH-dependent inhibition of native GABA(A) receptors by HEPES. Br J Pharmacol. 2012 Aug;166(8):2402-16. doi: 10.1111/j.1476-5381.2012.01956.x. PMID: 22452286; PMCID: PMC3448902.
In vivo protocol:	1. Nie J, Mahato S, Zelhof AC. Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation. BMC Dev Biol. 2015 Feb 3;15:10. doi: 10.1186/s12861-015-0056-y. PMID: 25645690; PMCID: PMC4320506.

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1: Arakawa T, Timasheff SN. The mechanism of action of Na glutamate, lysine HCl, and piperazine-N,N'-bis(2-ethanesulfonic acid) in the stabilization of tubulin and microtubule formation. J Biol Chem. 1984 Apr 25;259(8):4979-86. PMID: 6325414.

2: Roy RN, Gibbons JJ, Padron JL, Buechter K, Faszholz S, Doan H. Revised values of the paH of monosodium 1,4-piperazinediethanesulfonate ("Pipes') in water and other buffers in isotonic saline at various temperatures. Clin Chem. 1981 Oct;27(10):1787-8. PMID: 6269781.

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