Bromophenol red
featured

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 464244

CAS#: 2800-80-8

Description: Bromophenol red (BPR) is a chemical indicator. It has been shown to bind to lysozymes and inhibits their activity against bacterial cell walls, but not against the polysaccharide component of peptidoglycan.

Chemical Structure

img
Bromophenol red
CAS# 2800-80-8
Instruction

Theoretical Analysis

MedKoo Cat#: 464244
Name: Bromophenol red
CAS#: 2800-80-8
Chemical Formula: C19H12Br2O5S
Exact Mass: 509.88
Molecular Weight: 512.168
Elemental Analysis: C, 44.56; H, 2.36; Br, 31.20; O, 15.62; S, 6.26

Price and Availability

Size Price Availability Quantity
1g USD 250 2 Weeks
2g USD 400 2 Weeks
5g USD 700 2 Weeks
Bulk inquiry

Synonym: Bromophenol red; BPR;

IUPAC/Chemical Name: 3,3-bis(3-bromo-4-hydroxyphenyl)-3H-benzo[c][1,2]oxathiole 1,1-dioxide

InChi Key: OYCLSQDXZMROJK-UHFFFAOYSA-N

InChi Code: InChI=1S/C19H12Br2O5S/c20-14-9-11(5-7-16(14)22)19(12-6-8-17(23)15(21)10-12)13-3-1-2-4-18(13)27(24,25)26-19/h1-10,22-23H

SMILES Code: Oc1c(Br)cc(C2(c3cc(Br)c(O)cc3)OS(=O)(c4c2cccc4)=O)cc1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
To be determined 0.0 0.00

Preparing Stock Solutions

The following data is based on the product molecular weight 512.17 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Yu X, Sheng Y, Zhao Y, Fan A. Employment of bromophenol red and bovine serum albumin as luminol signal co-enhancer in chemiluminescent detection of sequence- specific DNA. Talanta. 2016;148:264-71. doi: 10.1016/j.talanta.2015.11.002. Epub 2015 Nov 3. PMID: 26653448.

2: Mikami D, Kurihara H, Kim SM, Takahashi K. Red algal bromophenols as glucose 6-phosphate dehydrogenase inhibitors. Mar Drugs. 2013 Oct 22;11(10):4050-7. doi: 10.3390/md11104050. PMID: 24152564; PMCID: PMC3826149.

3: Jittam P, Boonsiri P, Promptmas C, Sriwattanarothai N, Archavarungson N, Ruenwongsa P, Panijpan B. Red seaweed enzyme-catalyzed bromination of bromophenol red: An inquiry-based kinetics laboratory experiment for undergraduates. Biochem Mol Biol Educ. 2009 Mar;37(2):99-105. doi: 10.1002/bmb.20256. PMID: 21567713.

4: Madhusudan, Vijayan M. Additional binding sites in lysozyme. X-ray analysis of lysozyme complexes with bromophenol red and bromophenol blue. Protein Eng. 1992 Jul;5(5):399-404. doi: 10.1093/protein/5.5.399. PMID: 1518787.

5: Hunter-Cevera JC, Sotos L. Screening for a "new" enzyme in nature: Haloperoxidase production by Death Valley dematiaceous hyphomycetes. Microb Ecol. 1986 Mar;12(1):121-7. doi: 10.1007/BF02153227. PMID: 24212462.

6: Veerapandian B, Salunke DM, Vijayan M. X-ray characterisation of an additional binding site in lysozyme. FEBS Lett. 1985 Jul 8;186(2):163-7. doi: 10.1016/0014-5793(85)80701-8. PMID: 4007162.

7: Krishnamoorthy G, Prabhananda BS, Gurnani S. Bromophenol red as a probe of lysozyme-active site environment: a temperature-jump study. Biopolymers. 1979 Aug;18(8):1937-63. doi: 10.1002/bip.1979.360180809. PMID: 497350.