PHAD®-504
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 412664

CAS#: 1332714-01-8

Description: PHAD®-504 was designed as a synthetic structural analog of detoxified MPLA derived from E. coli lipopolysaccharide (LPS). It is structurally similar to PHAD®, differing only in the length of a single fatty acid chain. As expected, the activity of PHAD®-504 is quite similar to that of PHAD®, making the two products interchangeable as adjuvants in vaccine or immunotherapy formulations.

Chemical Structure

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PHAD®-504
CAS# 1332714-01-8
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 412664
Name: PHAD®-504
CAS#: 1332714-01-8
Chemical Formula: C94H180N3O22P
Exact Mass: 0.00
Molecular Weight: 1,735.447
Elemental Analysis: C, 65.06; H, 10.45; N, 2.42; O, 20.28; P, 1.78

Price and Availability

Size Price Availability Quantity
1mg USD 389.87 2 Weeks
5mg USD 1129.5 2 Weeks
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Synonym: 504; PHAD®-504; Monophosphoryl Lipid A-504

IUPAC/Chemical Name: ammonium (2R,3S,4R,5R,6R)-6-(((2R,3S,4R,5R,6S)-3,6-dihydroxy-5-((R)-3-hydroxytetradecanamido)-4-(((R)-3-hydroxytetradecanoyl)oxy)tetrahydro-2H-pyran-2-yl)methoxy)-5-((R)-3-(dodecanoyloxy)tetradecanamido)-2-(hydroxymethyl)-4-(((R)-3-(tetradecanoyloxy)tetradecanoyl)oxy)tetrahydro-2H-pyran-3-yl hydrogen phosphate

InChi Key: CYRPJUBABDDIFR-ADEWMWGLSA-N

InChi Code: InChI=1S/C94H177N2O22P.H3N/c1-7-13-19-25-31-37-38-44-50-56-62-68-84(103)113-78(66-60-54-48-42-35-29-23-17-11-5)72-86(105)117-92-88(96-82(101)71-77(65-59-53-47-41-34-28-22-16-10-4)112-83(102)67-61-55-49-43-36-30-24-18-12-6)94(115-79(73-97)90(92)118-119(108,109)110)111-74-80-89(106)91(116-85(104)70-76(99)64-58-52-46-40-33-27-21-15-9-3)87(93(107)114-80)95-81(100)69-75(98)63-57-51-45-39-32-26-20-14-8-2;/h75-80,87-94,97-99,106-107H,7-74H2,1-6H3,(H,95,100)(H,96,101)(H2,108,109,110);1H3/t75-,76-,77-,78-,79-,80-,87-,88-,89-,90-,91-,92-,93+,94-;/m1./s1

SMILES Code: O[C@H]([C@@H](CO[C@@H]([C@@H]1NC(C[C@H](OC(CCCCCCCCCCC)=O)CCCCCCCCCCC)=O)O[C@H](CO)[C@@H](OP([O-])(O)=O)[C@@H]1OC(C[C@H](OC(CCCCCCCCCCCCC)=O)CCCCCCCCCCC)=O)O[C@H](O)[C@@H]2NC(C[C@H](O)CCCCCCCCCCC)=O)[C@@H]2OC(C[C@H](O)CCCCCCCCCCC)=O.[NH4+]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Instruction:
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Biological target: PHAD®-504 was designed as a synthetic structural analog of detoxified MPLA derived from E. coli lipopolysaccharide (LPS). It is structurally similar to PHAD®, differing only in the length of a single fatty acid chain.
In vitro activity: TBD
In vivo activity: TBD

Preparing Stock Solutions

The following data is based on the product molecular weight 1,735.45 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: TBD
In vitro protocol: TBD
In vivo protocol: TBD

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1. Ji Y, An J, Hwang D, Ha DH, Lim SM, Lee C, Zhao J, Song HK, Yang EG, Zhou P, Chung HS. Metabolic engineering of Escherichia coli to produce a monophosphoryl lipid A adjuvant. Metab Eng. 2020 Jan;57:193-202. doi: 10.1016/j.ymben.2019.11.009. Epub 2019 Nov 28. PMID: 31786244; PMCID: PMC6960009.