Hydroxystilbamidine
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MedKoo CAT#: 574280

CAS#: 223769-64-0

Description: Hydroxystilbamidine is a fluorescent neuronal retrograde tracer that labels the neuronal cell body as well as proximal dendrites. It is also a nucleic acid dye that can be used to label DNA and RNA in fixed or unfixed dead cells. It displays an excitation maximum of 360 nm and emission maxima of 450 and 600 nm when bound to DNA but only emits at 450 nm when bound to RNA.

Chemical Structure

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Hydroxystilbamidine
CAS# 223769-64-0
Instruction

Theoretical Analysis

MedKoo Cat#: 574280
Name: Hydroxystilbamidine
CAS#: 223769-64-0
Chemical Formula: C18H24N4O7S2
Exact Mass: 472.11
Molecular Weight: 472.530
Elemental Analysis: C, 45.75; H, 5.12; N, 11.86; O, 23.70; S, 13.57

Price and Availability

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10mg USD 450
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Synonym: Hydroxystilbamidine

IUPAC/Chemical Name: 4-[2-[4-(aminoiminomethyl)phenyl]ethenyl]-3-hydroxy-benzenecarboximidamide, dimethanesulfonate

InChi Key: YGNSQKCULHSJDC-HFPMQDOPSA-N

InChi Code: InChI=1S/C16H16N4O.2CH4O3S/c17-15(18)12-5-2-10(3-6-12)1-4-11-7-8-13(16(19)20)9-14(11)21;2*1-5(2,3)4/h1-9,21H,(H3,17,18)(H3,19,20);2*1H3,(H,2,3,4)/b4-1+;;

SMILES Code: OC1=C(/C=C/C2=CC=C(C(N)=N)C=C2)C=CC(C(N)=N)=C1.O=S(C)(O)=O.O=S(C)(O)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info: Hydroxystilbamidine displays excitation/emission maxima of 323/620 nm, respectively.

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 472.53 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1. Akhavan, M., Hoang, T.X., and Havton, L.A. Improved detection of fluorogold-labeled neurons in long-term studies. J. Neurosci. Methods 152(1-2), 156-162 (2006).

2. Li, C., Marshall, C.T., Lu, C., et al. The dynamic distribution of fluoro-gold and its interrelation with neural nitric oxide synthase following intracerebroventricular injection into rat brain. Biotech. Histochem. 81(1), 41-50 (2006).

3. Festy, B., and Daune, M. Hydroxystilbamidine. A nonintercalating drug as a probe of nucleic acid conformation. Biochemistry 12(24), 4827-4834 (1973).