Light Green SF Yellowish | CAS#5141-20-8

Light Green SF Yellowish
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 330154

CAS#: 5141-20-8

Description: Light green SF yellowish (C.I. 42095) is a dye that is used for the preparation of a staining solution which is is widely used as a counterstain. for e.g. trichrome Masson-Goldner staining in histological sections or for Papanicolaou’s polychromatic staining in cytology of samples human origin

Chemical Structure

Light Green SF Yellowish

CAS# 5141-20-8

Product data Instruction

Theoretical Analysis

MedKoo Cat#: 330154
Name: Light Green SF Yellowish
CAS#: 5141-20-8
Chemical Formula: C37H34N2Na2O9S3
Exact Mass: 0.00
Molecular Weight: 792.844
Elemental Analysis: C, 56.05; H, 4.32; N, 3.53; Na, 5.80; O, 18.16; S, 12.13

Price and Availability

Size	Price	Availability
5g	USD 250	2 Weeks
25g	USD 450	2 Weeks
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: Light Green SF Yellowish; Acid Green 5; C.I. 42095; C.I. Acid Green 5;

IUPAC/Chemical Name: sodium 3-((ethyl((1E,4E)-4-((4-(ethyl(3-sulfonatobenzyl)amino)phenyl)(4-sulfonatophenyl)methylene)cyclohexa-2,5-dien-1-ylidene)ammonio)methyl)benzenesulfonate

InChi Key: DGOBMKYRQHEFGQ-UHFFFAOYSA-L

InChi Code: InChI=1S/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2

SMILES Code: O=S(C1=CC=CC(C/[N+](CC)=C2C=C/C(C=C\2)=C(C3=CC=C(N(CC)CC4=CC=CC(S(=O)([O-])=O)=C4)C=C3)/C5=CC=C(S(=O)([O-])=O)C=C5)=C1)([O-])=O.[Na+].[Na+]

Appearance: Dark Red to Purple Solid powder

Purity: >70% dye content (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in water (200mg/ mL)

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 3204 19 00

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Product Data:

Product Data

Instruction:

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Biological target:	Light green SF yellowish (Acid Green 5) is a triarylmethane dye.
In vitro activity:	The remaining two potential inhibitors were light green SF yellowish (45% decrease) and β-cyclodextrin (β-CD, 75% decrease). Dose-response analyses confirmed both of these excipients as modest inhibitors of digoxin flux, with IC50 estimates of 168 μM (95% CI, 118–251 μM, Figure 3D) and 204 μM (95% CI, 5.9–1745 μM, Figure 3E) for β-CD and light green SF yellowish, respectively (Figures 3D and 3E). Reference: AAPS J. 2021 Sep 15;23(5):106. https://pubmed.ncbi.nlm.nih.gov/34528148/
In vivo activity:	Four dyes in different solutions (light green SF yellowish [LGSF]: 2%; copper(II) phthalocyanine-tetrasulfonic acid [E68]: 2% and 0.5%; bromophenol blue [BPB]: 2%, 1%, and 0.2%; and Chicago blue [CB]: 2% and 0.5%) were included in this investigation. After triamcinolone-assisted vitrectomy on 10 porcine eyes in vivo, the dyes were first injected into the air-filled vitreous cavity. No staining of the retinal surface but of the vitreous was seen after application of LGSF 2%. Reference: Retina. 2006 Jul-Aug;26(6):673-8. https://pubmed.ncbi.nlm.nih.gov/16829811/

Preparing Stock Solutions

The following data is based on the product molecular weight 792.84 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:	1. Bajaj R, Chong LB, Zou L, Tsakalozou E, Ni Z, Giacomini KM, Kroetz DL. Interaction of Commonly Used Oral Molecular Excipients with P-glycoprotein. AAPS J. 2021 Sep 15;23(5):106. doi: 10.1208/s12248-021-00631-8. PMID: 34528148; PMCID: PMC9148196. 2. Haddad A, Merouani S, Hannachi C, Hamdaoui O, Hamrouni B. Intensification of light green SF yellowish (LGSFY) photodegradion in water by iodate ions: Iodine radicals implication in the degradation process and impacts of water matrix components. Sci Total Environ. 2019 Feb 20;652:1219-1227. doi: 10.1016/j.scitotenv.2018.10.183. Epub 2018 Oct 15. PMID: 30586808. 3. Haritoglou C, Tadayoni R, May CA, Gass CA, Freyer W, Priglinger SG, Kampik A. Short-term in vivo evaluation of novel vital dyes for intraocular surgery. Retina. 2006 Jul-Aug;26(6):673-8. doi: 10.1097/01.iae.0000236505.42892.54. PMID: 16829811.
In vitro protocol:	1. Bajaj R, Chong LB, Zou L, Tsakalozou E, Ni Z, Giacomini KM, Kroetz DL. Interaction of Commonly Used Oral Molecular Excipients with P-glycoprotein. AAPS J. 2021 Sep 15;23(5):106. doi: 10.1208/s12248-021-00631-8. PMID: 34528148; PMCID: PMC9148196. 2. Haddad A, Merouani S, Hannachi C, Hamdaoui O, Hamrouni B. Intensification of light green SF yellowish (LGSFY) photodegradion in water by iodate ions: Iodine radicals implication in the degradation process and impacts of water matrix components. Sci Total Environ. 2019 Feb 20;652:1219-1227. doi: 10.1016/j.scitotenv.2018.10.183. Epub 2018 Oct 15. PMID: 30586808.
In vivo protocol:	1. Haritoglou C, Tadayoni R, May CA, Gass CA, Freyer W, Priglinger SG, Kampik A. Short-term in vivo evaluation of novel vital dyes for intraocular surgery. Retina. 2006 Jul-Aug;26(6):673-8. doi: 10.1097/01.iae.0000236505.42892.54. PMID: 16829811.

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