Chromozym TH

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 596872

CAS#: 61876-61-7

Description: Chromozym TH is a synthetic substrate for thrombin which acts on it to liberate p-nitroaniline (yellow color).

Chemical Structure

img
Chromozym TH
CAS# 61876-61-7
Instruction

Theoretical Analysis

MedKoo Cat#: 596872
Name: Chromozym TH
CAS#: 61876-61-7
Chemical Formula: C26H35ClN8O7S
Exact Mass: 0.00
Molecular Weight: 639.120
Elemental Analysis: C, 48.86; H, 5.52; Cl, 5.55; N, 17.53; O, 17.52; S, 5.02

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

Request quote for custom synthesis

Synonym: Chromozym TH; Chr-TH; Tos-gly-pro-arg-p-nitroanilide; Tos-gly-pro-arg-pna;

IUPAC/Chemical Name: (S)-N-((S)-1-amino-5-guanidino-1-oxopentan-2-yl)-N-(4-nitrophenyl)-1-(tosylglycyl)pyrrolidine-2-carboxamide hydrochloride

InChi Key: LPPYQFMRWDVWAK-VROPFNGYSA-N

InChi Code: InChI=1S/C26H34N8O7S.ClH/c1-17-6-12-20(13-7-17)42(40,41)31-16-23(35)32-15-3-5-22(32)25(37)33(18-8-10-19(11-9-18)34(38)39)21(24(27)36)4-2-14-30-26(28)29;/h6-13,21-22,31H,2-5,14-16H2,1H3,(H2,27,36)(H4,28,29,30);1H/t21-,22-;/m0./s1

SMILES Code: N=C(N)NCCC[C@@H](C(N)=O)N(C([C@H]1N(C(CNS(=O)(C2=CC=C(C)C=C2)=O)=O)CCC1)=O)C3=CC=C([N+]([O-])=O)C=C3.[H]Cl

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 639.12 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Wang JY, Hou WZ, Wei YJ, Gao SS, Bai GC, Wang AD, Ma CH. [A new method on investigate chemical constituents which have anti-thrombin effect by HPLC]. Zhongguo Zhong Yao Za Zhi. 2016 Aug;41(15):2855-2860. doi: 10.4268/cjcmm20161518. Chinese. PubMed PMID: 28914028.

2: Bharadwaz M, Manna P, Das D, Dutta N, Kalita J, Unni B, Deka Boruah HP. Isolation, purification, and characterization of staphylocoagulase, a blood coagulating protein from Staphylococcus sp. MBBJP S43. Int J Biol Macromol. 2017 Sep;102:1312-1321. doi: 10.1016/j.ijbiomac.2017.05.005. Epub 2017 May 2. PubMed PMID: 28476593.

3: Jiang SY, Jiao J, Lu J, Xu YP, Dou GF. [Determination of recombinant hirudin in urine of rat by spectrophotometric method]. Guang Pu Xue Yu Guang Pu Fen Xi. 2013 May;33(5):1327-30. Chinese. PubMed PMID: 23905345.

4: Lu W, Cai X, Gu Z, Huang Y, Xia B, Cao P. Production and characterization of hirudin variant-1 by SUMO fusion technology in E. coli. Mol Biotechnol. 2013 Jan;53(1):41-8. doi: 10.1007/s12033-012-9511-1. PubMed PMID: 22371262.

5: Pan R, Image I, Zhang XJ, Image II, Zhang ZJ, Image II, Zhou Y, Image I, Tian WX, Image II, He RQ, Image II. Substrate-induced changes in protease active site conformation impact on subsequent reactions with substrates. J Biol Chem. 2010 Jul 23;285(30):22950-6. doi: 10.1074/jbc.M110.103549. Epub 2010 May 18. PubMed PMID: 20484054; PubMed Central PMCID: PMC2906288.

6: Chao J, Huang WY, Wang J, Xiao SJ, Tang YC, Liu JN. Click-chemistry-conjugated oligo-angiomax in the two-dimensional DNA lattice and its interaction with thrombin. Biomacromolecules. 2009 Apr 13;10(4):877-83. doi: 10.1021/bm8014076. PubMed PMID: 19275167.

7: Makarova AM, Zamolodchikova TS, Rumsh LD, Strukova SM. [Duodenase activates rat peritoneal mast cells via protease-activated receptors of type 1]. Bioorg Khim. 2007 Sep-Oct;33(5):520-6. Russian. PubMed PMID: 18050657.

8: Poiarkov AA, Poiarkova SA, Kukhar' VP. [Antithrombic activity of N(alpha)-lauroyl-L-arginine ethyl ester (LAE) and 9-fluorenylmethoxycarbonyl-L-arginine methyl ester (Fmoc-L-Arg-OMe)]. Ukr Biokhim Zh (1999). 2007 Jan-Feb;79(1):124-31. Russian. PubMed PMID: 18030741.

9: Tebbe D, Thull R, Gbureck U. Influence of spacer length on heparin coupling efficiency and fibrinogen adsorption of modified titanium surfaces. Biomed Eng Online. 2007 Jul 17;6:31. PubMed PMID: 17640335; PubMed Central PMCID: PMC1950714.

10: Graf LL, Young DA, Kressin DC, Marlar RA, Jacob GB, Hinderling PH. Importance of using controls in in vivo experiments. Radiology. 2004 Oct;233(1):297-8; author reply 298-9. PubMed PMID: 15454626.

11: Zhao J, Li L, Wu C, He RQ. Hydrolysis of fibrinogen and plasminogen by immobilized earthworm fibrinolytic enzyme II from Eisenia fetida. Int J Biol Macromol. 2003 Sep;32(3-5):165-71. PubMed PMID: 12957313.

12: Bodendiek I, Lentz S, Seeger M, Bruhn HD. [Chromogenic substrate as antidote against the thrombin inhibitor Melagatran?]. Hamostaseologie. 2003 May;23(2):97-8. German. PubMed PMID: 12736706.

13: Li L, Zhao J, He RQ. Isolation and some characterizations of a glycosylated fibrinolytic enzyme of earthworm, Eisenia fetida. Protein Pept Lett. 2003 Apr;10(2):183-90. PubMed PMID: 12678816.

14: Cappiello M, Vilardo PG, Lippi A, Criscuoli M, Del Corso A, Mura U. Kinetics of human thrombin inhibition by two novel peptide inhibitors (Hirunorm IV and Hirunorm V). Biochem Pharmacol. 1996 Oct 25;52(8):1141-6. PubMed PMID: 8937420.

15: Solov'ev DA, Platonova TN, Ugarova TP. [Isolation and characteristics of ekamulin--a prothrombin activator from multiscaled viper (Echis multisquamatus) venom]. Biokhimiia. 1996 Jun;61(6):1094-105. Russian. PubMed PMID: 9011245.

16: Mast AE, Broze GJ Jr. Physiological concentrations of tissue factor pathway inhibitor do not inhibit prothrombinase. Blood. 1996 Mar 1;87(5):1845-50. PubMed PMID: 8634431.

17: Picozzi M, Landolfi R, De Cristofaro R. Effects of protons on the thrombin-fibrinogen interaction. Eur J Biochem. 1994 Feb 1;219(3):1013-21. PubMed PMID: 8112314.

18: Mathur A, Schlapkohl WA, Di Cera E. Thrombin-fibrinogen interaction: pH dependence and effects of the slow-->fast transition. Biochemistry. 1993 Jul 27;32(29):7568-73. PubMed PMID: 8393345.

19: Rásky K, Pátfalusi M, Vereczkey L. Chromogen-substrate assay--as a tool for monitoring a new thrombin inhibitor. Acta Pharm Hung. 1993 Jan;63(1):3-12. PubMed PMID: 8452033.

20: Lewis SD, Brezniak DV, Fenton JW 2nd, Shafer JA. Catalytically competent human and bovine zeta-thrombin and chimeras generated from unfolded polypeptide chains. Protein Sci. 1992 Aug;1(8):998-1006. PubMed PMID: 1304387; PubMed Central PMCID: PMC2142167.