SMER3
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 562487

CAS#: 67200-34-4

Description: SMER3 is a small molecule enhancer of rapamycin (SMER). It is also a specific inhibitor of Ubiquitin E3 ligase.

Chemical Structure

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SMER3
CAS# 67200-34-4
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 562487
Name: SMER3
CAS#: 67200-34-4
Chemical Formula: C11H4N4O2
Exact Mass: 224.03
Molecular Weight: 224.180
Elemental Analysis: C, 58.94; H, 1.80; N, 24.99; O, 14.27

Price and Availability

Size Price Availability Quantity
5mg USD 220
10mg USD 350
25mg USD 620
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Synonym: SMER3; SMER-3; SMER 3;

IUPAC/Chemical Name: 9H-Indeno-[1,2-e]-[1,2,5]-oxadiazolo-[3,4-b]-pyrazin-9-one

InChi Key: SFSSAKVWCKFRHE-UHFFFAOYSA-N

InChi Code: InChI=1S/C11H4N4O2/c16-9-6-4-2-1-3-5(6)7-8(9)13-11-10(12-7)14-17-15-11/h1-4H

SMILES Code: O=C1C2=C(C3=NC4=NON=C4N=C31)C=CC=C2

Appearance: Solid powder

Purity: >95% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Certificate of Analysis:
View CoA: current batch, Lot#C9M04C17
Safety Data Sheet (SDS):
View Safety Data Sheet (SDS)
Instruction:
View handling instruction
Biological target: SMER3 is a selective inhibitor of a yeast SCF family E3 ubiquitin ligase (SCFMet30) in vitro and in vivo. SMER3 induces the expression of MET genes and blocks cell proliferation. SMER3 is also an enhancer of rapamycin.
In vitro activity: To be determined
In vivo activity: This study discovered SMER3, which regulates diverse cellular processes including transcription, cell-cycle control and immune response. SMER3 inhibited SCF(Met30) in vivo and in vitro, but not the closely related SCF(Cdc4). SMER3 also diminished binding of the F-box subunit Met30 to the SCF core complex in vivo and show evidence for SMER3 directly binding to Met30. Reference: Nat Biotechnol. 2010 Jul;28(7):738-42. https://pubmed.ncbi.nlm.nih.gov/20581845/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 16.8 75.00

Preparing Stock Solutions

The following data is based on the product molecular weight 224.18 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Aghajan M, Jonai N, Flick K, Fu F, Luo M, Cai X, Ouni I, Pierce N, Tang X, Lomenick B, Damoiseaux R, Hao R, Del Moral PM, Verma R, Li Y, Li C, Houk KN, Jung ME, Zheng N, Huang L, Deshaies RJ, Kaiser P, Huang J. Chemical genetics screen for enhancers of rapamycin identifies a specific inhibitor of an SCF family E3 ubiquitin ligase. Nat Biotechnol. 2010 Jul;28(7):738-42. doi: 10.1038/nbt.1645. Epub 2010 Jun 27. PMID: 20581845; PMCID: PMC2902569.
In vitro protocol: To be determined
In vivo protocol: 1. Aghajan M, Jonai N, Flick K, Fu F, Luo M, Cai X, Ouni I, Pierce N, Tang X, Lomenick B, Damoiseaux R, Hao R, Del Moral PM, Verma R, Li Y, Li C, Houk KN, Jung ME, Zheng N, Huang L, Deshaies RJ, Kaiser P, Huang J. Chemical genetics screen for enhancers of rapamycin identifies a specific inhibitor of an SCF family E3 ubiquitin ligase. Nat Biotechnol. 2010 Jul;28(7):738-42. doi: 10.1038/nbt.1645. Epub 2010 Jun 27. PMID: 20581845; PMCID: PMC2902569.

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1: Aghajan M, Jonai N, Flick K, Fu F, Luo M, Cai X, Ouni I, Pierce N, Tang X, Lomenick B, Damoiseaux R, Hao R, Del Moral PM, Verma R, Li Y, Li C, Houk KN, Jung ME, Zheng N, Huang L, Deshaies RJ, Kaiser P, Huang J. Chemical genetics screen for enhancers of rapamycin identifies a specific inhibitor of an SCF family E3 ubiquitin ligase. Nat Biotechnol. 2010 Jul;28(7):738-42. doi: 10.1038/nbt.1645. Epub 2010 Jun 27. PubMed PMID: 20581845; PubMed Central PMCID: PMC2902569.