NIH-12848 | CAS#959551-10-1 | PI5P4Kγ) inhibitor

NIH-12848
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 530601

CAS#: 959551-10-1

Description: NIH-12848 is a putative phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) inhibitor. NIH-12848 inhibited PI5P4Kγ with an IC50 of approximately 1 μM but did not inhibit the α and β PI5P4K isoforms at concentrations up to 100 μM. NIH-12848 is a potentially powerful tool for exploring the cell physiology of PI5P4Ks.

Chemical Structure

NIH-12848

CAS# 959551-10-1

Product data Instruction

Theoretical Analysis

MedKoo Cat#: 530601
Name: NIH-12848
CAS#: 959551-10-1
Chemical Formula: C20H14F3N3S
Exact Mass: 385.09
Molecular Weight: 385.408
Elemental Analysis: C, 62.33; H, 3.66; F, 14.79; N, 10.90; S, 8.32

Price and Availability

Size	Price	Availability	Quantity
100mg	USD 850
200mg	USD 1250
500mg	USD 2150
1g	USD 3450
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: NIH-12848; NIH 12848; NIH12848, NCGC00012848-02.

IUPAC/Chemical Name: N-(thiophen-2-ylmethyl)-2-(2-(trifluoromethyl)phenyl)quinazolin-4-amine

InChi Key: QBDAEJRHUCSSPR-UHFFFAOYSA-N

InChi Code: InChI=1S/C20H14F3N3S/c21-20(22,23)16-9-3-1-7-14(16)19-25-17-10-4-2-8-15(17)18(26-19)24-12-13-6-5-11-27-13/h1-11H,12H2,(H,24,25,26)

SMILES Code: FC(F)(F)C(C=CC=C1)=C1C2=NC3=CC=CC=C3C(NCC4=CC=CS4)=N2

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

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Product Data:

Product Data

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Biological target:	NIH-12848 is a putative phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) inhibitor with an IC50 of 1 μM.
In vitro activity:	NIH-12848 (10 μM) was applied to cultured mouse principal kidney cortical collecting duct (mpkCCD) cells which, we show, express PI5P4Kγ that increases when the cells grow to confluence and polarize. NIH-12848 inhibited the translocation of Na⁺/K⁺-ATPase to the plasma membrane that occurs when mpkCCD cells grow to confluence and also prevented reversibly their forming of 'domes' on the culture dish. Reference: Biochem J. 2015 Mar 1;466(2):359-67. https://pubmed.ncbi.nlm.nih.gov/25495341/
In vivo activity:	TBD

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	67.3	174.71
	Ethanol	77.0	200.00

Preparing Stock Solutions

The following data is based on the product molecular weight 385.41 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:	Clarke JH, Giudici ML, Burke JE, Williams RL, Maloney DJ, Marugan J, Irvine RF. The function of phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) explored using a specific inhibitor that targets the PI5P-binding site. Biochem J. 2015 Mar 1;466(2):359-67. doi: 10.1042/BJ20141333. PMID: 25495341; PMCID: PMC4687057.
In vitro protocol:	Clarke JH, Giudici ML, Burke JE, Williams RL, Maloney DJ, Marugan J, Irvine RF. The function of phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) explored using a specific inhibitor that targets the PI5P-binding site. Biochem J. 2015 Mar 1;466(2):359-67. doi: 10.1042/BJ20141333. PMID: 25495341; PMCID: PMC4687057.
In vivo protocol:	TBD

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1: Clarke JH, Giudici ML, Burke JE, Williams RL, Maloney DJ, Marugan J, Irvine
RF. The function of phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ)
explored using a specific inhibitor that targets the PI5P-binding site. Biochem
J. 2015 Mar 1;466(2):359-67. doi: 10.1042/BJ20141333. PubMed PMID: 25495341;
PubMed Central PMCID: PMC4687057.

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