CM-414

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 526875

CAS#: 1624792-70-6 (cis-isomer)

Description: CM-414 is a dual inhibitor of PDE5 and HDACs for the Treatment of Alzheimer’s Disease (IC50 values of 60 nM, 310 nM, 490 nM, 322 nM, and 91 nM against PDE5, HDAC1, HDAC2, HDAC3, and HDAC6, respectively). Chronic treatment of Tg2576 mice with CM-414 diminished brain Aβ and tau phosphorylation (pTau) levels, increased the inactive form of GSK3β, reverted the decrease in dendritic spine density on hippocampal neurons, and reversed their cognitive deficits, at least in part by inducing the expression of genes related to synaptic transmission. Thus, CM-414 may serve as the starting point to discover balanced dual inhibitors with an optimal efficacy and safety profile for clinical testing on AD patients.

Chemical Structure

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CM-414
CAS# 1624792-70-6 (cis-isomer)
Instruction

Theoretical Analysis

MedKoo Cat#: 526875
Name: CM-414
CAS#: 1624792-70-6 (cis-isomer)
Chemical Formula: C23H29N5O4
Exact Mass: 439.22
Molecular Weight: 439.516
Elemental Analysis: C, 62.85; H, 6.65; N, 15.93; O, 14.56

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: CM-414; CM 414; CM414.

IUPAC/Chemical Name: 3-(4-ethoxy-3-(1-methyl-7-oxo-3-propyl-6,7-dihydro-1H-pyrazolo[4,3-d]pyrimidin-5-yl)benzyl)-N-hydroxycyclobutane-1-carboxamide

InChi Key: GTSZATFSVLYIRU-UHFFFAOYSA-N

InChi Code: InChI=1S/C23H29N5O4/c1-4-6-17-19-20(28(3)26-17)23(30)25-21(24-19)16-12-13(7-8-18(16)32-5-2)9-14-10-15(11-14)22(29)27-31/h7-8,12,14-15,31H,4-6,9-11H2,1-3H3,(H,27,29)(H,24,25,30)

SMILES Code: CCOC1=C(C2=NC(C(CCC)=NN3C)=C3C(N2)=O)C=C(CC4CC(C(NO)=O)C4)C=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info: Related CAS# 1624792-70-6 (CM-414 cis-isomer) 1624792-73-9 (CM-414 trans-isomer) CM-414 can cross the blood−brain barrier (BBB), inducing AcH3K9 acetylation and CREB phosphorylation in the hippocampus and rescuing long-term potentiation (LTP) in APP/PS1 mice. Simultaneous inhibition of phosphodiesterase 5 (PDE5) and histone deacetylases (HDAC) has recently been validated as a potentially novel therapeutic approach for Alzheimer’s disease (AD).

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 439.52 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Cuadrado-Tejedor M, Garcia-Barroso C, Sánchez-Arias JA, Rabal O,
Pérez-González M, Mederos S, Ugarte A, Franco R, Segura V, Perea G, Oyarzabal J,
Garcia-Osta A. A First-in-Class Small-Molecule that Acts as a Dual Inhibitor of
HDAC and PDE5 and that Rescues Hippocampal Synaptic Impairment in Alzheimer's
Disease Mice. Neuropsychopharmacology. 2016 Sep 21. doi: 10.1038/npp.2016.163.
[Epub ahead of print] PubMed PMID: 27550730.