WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 526644

CAS#: 104821-25-2

Description: Dihydroethidium, also known as Hydroethidine and PD-MY 003, is a cell-permeable blue fluorescent dye. DHE-derived fluorescence at 570 nm is a convenient method for detection of intracellular and extracellular superoxide produced by phagocytic and vascular NADPH oxidase. Dihydroethidium is neuroprotective by reducing superoxide in mice after stroke.

Price and Availability

Size Price Shipping out time Quantity
100mg USD 550 2 weeks
200mg USD 950 2 weeks
500mg USD 1650 2 weeks
1g USD 2450 2 weeks
2g USD 3650 2 weeks
5g USD 5450 2 weeks
Inquire bulk and customized quantity

Pricing updated 2021-01-20. Prices are subject to change without notice.

Dihydroethidium, purity > 98%, is in stock. Current shipping out time is about 2 weeks after order is received. CoA, QC data and MSDS documents are available in one week after order is received.

Chemical Structure


Theoretical Analysis

MedKoo Cat#: 526644
Name: Dihydroethidium
CAS#: 104821-25-2
Chemical Formula: C21H21N3
Exact Mass: 315.1735
Molecular Weight: 315.42
Elemental Analysis: C, 79.97; H, 6.71; N, 13.32

Synonym: PD-MY 003; PD-MY-003; PD-MY003; Hydroethidine; Dihydroethidium

IUPAC/Chemical Name: 5-ethyl-5,6-dihydro-6-phenyl-3,8-phenanthridinediamine


InChi Code: InChI=1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3

SMILES Code: NC1=CC=C2C3=C(C=C(N)C=C3)C(C4=CC=CC=C4)N(CC)C2=C1

Technical Data

Solid powder

>98% (or refer to the Certificate of Analysis)

Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition:
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Soluble in DMSO, not in water

Shelf Life:
>2 years if stored properly

Drug Formulation:
This drug may be formulated in DMSO

Stock Solution Storage:
0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code:


1: Michalski R, Michalowski B, Sikora A, Zielonka J, Kalyanaraman B. On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: a reassessment. Free Radic Biol Med. 2014 Feb;67:278-84. doi: 10.1016/j.freeradbiomed.2013.10.816. Epub 2013 Nov 5. PubMed PMID: 24200598; PubMed Central PMCID: PMC4275029.

2: Chen J, Rogers SC, Kavdia M. Analysis of kinetics of dihydroethidium fluorescence with superoxide using xanthine oxidase and hypoxanthine assay. Ann Biomed Eng. 2013 Feb;41(2):327-37. doi: 10.1007/s10439-012-0653-x. Epub 2012 Sep 11. PubMed PMID: 22965641; PubMed Central PMCID: PMC3544990.

3: Laurindo FR, Fernandes DC, Santos CX. Assessment of superoxide production and NADPH oxidase activity by HPLC analysis of dihydroethidium oxidation products. Methods Enzymol. 2008;441:237-60. doi: 10.1016/S0076-6879(08)01213-5. Review. PubMed PMID: 18554538.

4: Peshavariya HM, Dusting GJ, Selemidis S. Analysis of dihydroethidium fluorescence for the detection of intracellular and extracellular superoxide produced by NADPH oxidase. Free Radic Res. 2007 Jun;41(6):699-712. PubMed PMID: 17516243.

5: Burnaugh L, Sabeur K, Ball BA. Generation of superoxide anion by equine spermatozoa as detected by dihydroethidium. Theriogenology. 2007 Feb;67(3):580-9. Epub 2006 Oct 12. PubMed PMID: 17045638.

6: Fink B, Laude K, McCann L, Doughan A, Harrison DG, Dikalov S. Detection of intracellular superoxide formation in endothelial cells and intact tissues using dihydroethidium and an HPLC-based assay. Am J Physiol Cell Physiol. 2004 Oct;287(4):C895-902. Epub 2004 Aug 11. PubMed PMID: 15306539.

7: Yu F, Sugawara T, Chan PH. Treatment with dihydroethidium reduces infarct size after transient focal cerebral ischemia in mice. Brain Res. 2003 Jul 18;978(1-2):223-7. PubMed PMID: 12834917.