WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 526618

CAS#: 164025-33-6

Description: PWZ-029 IS an inverse agonist selective for α₅ GABAA receptors with nootropic effects. PWZ-029 improves object recognition, but not water-maze memory in normal and scopolamine-treated rats. PWZ-029 acts as a subtype-selective, mixed agonist-inverse agonist at the benzodiazepine binding site on the GABAA receptor, acting as a partial inverse agonist at the α5 subtype and a weak partial agonist at the α3 subtype. This gives it a mixed pharmacological profile, producing at low doses memory-enhancing effects but with no convulsant or anxiogenic effects or muscle weakness, although at higher doses it produces some sedative effects

Chemical Structure

CAS# 164025-33-6

Theoretical Analysis

MedKoo Cat#: 526618
Name: PWZ-029
CAS#: 164025-33-6
Chemical Formula: C14H14ClN3O2
Exact Mass: 291.08
Molecular Weight: 291.735
Elemental Analysis: C, 57.64; H, 4.84; Cl, 12.15; N, 14.40; O, 10.97

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales or click below button.
Note: Price will be listed if it is available in the future.

Synonym: PWZ-029; PWZ 029; PWZ029.

IUPAC/Chemical Name: 8-chloro-3-(methoxymethyl)-5-methyl-4H-imidazo[1,5-a][1,4]benzodiazepin-6-one


InChi Code: InChI=1S/C14H14ClN3O2/c1-17-6-13-11(7-20-2)16-8-18(13)12-4-3-9(15)5-10(12)14(17)19/h3-5,8H,6-7H2,1-2H3


Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, not in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Product Data:

Preparing Stock Solutions

The following data is based on the product molecular weight 291.735000000000000000000000000000 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

1: Clayton T, Poe MM, Rallapalli S, Biawat P, Savić MM, Rowlett JK, Gallos G, Emala CW, Kaczorowski CC, Stafford DC, Arnold LA, Cook JM. A Review of the Updated Pharmacophore for the Alpha 5 GABA(A) Benzodiazepine Receptor Model. Int J Med Chem. 2015;2015:430248. doi: 10.1155/2015/430248. Epub 2015 Nov 10. Review. PubMed PMID: 26682068; PubMed Central PMCID: PMC4657098. 2: Timić Stamenić T, Joksimović S, Biawat P, Stanković T, Marković B, Cook JM, Savić MM. Negative modulation of α₅ GABAA receptors in rats may partially prevent memory impairment induced by MK-801, but not amphetamine- or MK-801-elicited hyperlocomotion. J Psychopharmacol. 2015 Sep;29(9):1013-24. doi: 10.1177/0269881115590601. Epub 2015 Jun 23. PubMed PMID: 26105958. 3: Martínez-Cué C, Delatour B, Potier MC. Treating enhanced GABAergic inhibition in Down syndrome: use of GABA α5-selective inverse agonists. Neurosci Biobehav Rev. 2014 Oct;46 Pt 2:218-27. doi: 10.1016/j.neubiorev.2013.12.008. Epub 2014 Jan 9. Review. PubMed PMID: 24412222. 4: Soto PL, Ator NA, Rallapalli SK, Biawat P, Clayton T, Cook JM, Weed MR. Allosteric modulation of GABA(A) receptor subtypes:effects on visual recognition and visuospatial working memory in rhesus monkeys [corrected]. Neuropsychopharmacology. 2013 Oct;38(11):2315-25. doi: 10.1038/npp.2013.137. Epub 2013 May 31. Erratum in: Neuropsychopharmacology. 2013 Nov;38(12):2553. PubMed PMID: 23722241; PubMed Central PMCID: PMC3773684. 5: Milić M, Timić T, Joksimović S, Biawat P, Rallapalli S, Divljaković J, Radulović T, Cook JM, Savić MM. PWZ-029, an inverse agonist selective for α₅ GABAA receptors, improves object recognition, but not water-maze memory in normal and scopolamine-treated rats. Behav Brain Res. 2013 Mar 15;241:206-13. doi: 10.1016/j.bbr.2012.12.016. Epub 2012 Dec 20. PubMed PMID: 23261875; PubMed Central PMCID: PMC3556169. 6: Savić MM, Clayton T, Furtmüller R, Gavrilović I, Samardzić J, Savić S, Huck S, Sieghart W, Cook JM. PWZ-029, a compound with moderate inverse agonist functional selectivity at GABA(A) receptors containing alpha5 subunits, improves passive, but not active, avoidance learning in rats. Brain Res. 2008 May 7;1208:150-9. doi: 10.1016/j.brainres.2008.02.020. Epub 2008 Feb 19. PubMed PMID: 18394590; PubMed Central PMCID: PMC2577822.