L-allo-Isoleucine | CAS#1509-34-8 | MSUD

L-allo-Isoleucine
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 620106

CAS#: 1509-34-8

Description: L-allo-Isoleucine may be used in studies on maple syrup urine disease (MSUD). In patients with classical MSUD (n = 7), alloisoleucine was beyond the cutoff value in 2451 of 2453 unselected samples. In patients with variant MSUD (n = 9), alloisoleucine was >5 micromol/L in all samples taken for establishment of diagnosis and in 94% of the samples taken for treatment control (n = 624). With the other branched-chain amino acids, the frequency of diagnostically significant increases was <45%. The plasma L-alloisoleucine above the cutoff value of 5 micromol/L is the most specific and most sensitive diagnostic marker for all forms of MSUD.

Chemical Structure

L-allo-Isoleucine

CAS# 1509-34-8

Product data Instruction

Theoretical Analysis

MedKoo Cat#: 620106
Name: L-allo-Isoleucine
CAS#: 1509-34-8
Chemical Formula: C6H13NO2
Exact Mass: 131.09
Molecular Weight: 131.175
Elemental Analysis: C, 54.94; H, 9.99; N, 10.68; O, 24.39

Price and Availability

Size	Price	Availability
250mg	USD 285	2 Weeks
500mg	USD 450
1g	USD 650
2g	USD 950	2 Weeks
5g	USD 1850	2 Weeks
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: L-allo-Isoleucine; L-alloIsoleucine.

IUPAC/Chemical Name: (2S,3R)-2-Amino-3-methylpentanoic acid

InChi Key: AGPKZVBTJJNPAG-UHNVWZDZSA-N

InChi Code: InChI=1S/C6H13NO2/c1-3-4(2)5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t4-,5+/m1/s1

SMILES Code: CC[C@@H](C)[C@H](N)C(O)=O

Appearance: Solid powder

Purity: >97% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, not in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:

Product Data

Instruction:

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Biological target:	L-Alloisoleucine is a branched chain amino acid and is a stereo-isomer of L-isoleucine.
In vitro activity:	This study used the Maple syrup urine disease (MSUD) biomarker L-allo-isoleucine to test the selection model. After five rounds of screening, the cleavage products were sufficiently enriched to be visualized on polyacrylamide gel electrophoresis (PAGE) gel. Through high-throughput sequencing analysis, several candidates were identified. One such candidate, IR3-I-DNA, binds L-allo-isoleucine with a dissociation constant (K D) of 0.57 mM. Reference: ACS Omega. 2022 Mar 17;7(12):10804-10811. https://pubmed.ncbi.nlm.nih.gov/35382297/
In vivo activity:	TBD

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	PBS (pH 7.2)	1.0	7.62
	Water	10.0	76.24

Preparing Stock Solutions

The following data is based on the product molecular weight 131.18 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:	1. Ao Y, Duan A, Chen B, Yu X, Wu Y, Zhang X, Li S. Integration of an Expression Platform in the SELEX Cycle to Select DNA Aptamer Binding to a Disease Biomarker. ACS Omega. 2022 Mar 17;7(12):10804-10811. doi: 10.1021/acsomega.2c00769. PMID: 35382297; PMCID: PMC8973154.
In vitro protocol:	1. Ao Y, Duan A, Chen B, Yu X, Wu Y, Zhang X, Li S. Integration of an Expression Platform in the SELEX Cycle to Select DNA Aptamer Binding to a Disease Biomarker. ACS Omega. 2022 Mar 17;7(12):10804-10811. doi: 10.1021/acsomega.2c00769. PMID: 35382297; PMCID: PMC8973154.
In vivo protocol:	TBD

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1: Rodrigues KT, Mekahli D, Tavares MF, Van Schepdael A. Development and validation of a CE-MS method for the targeted assessment of amino acids in urine. Electrophoresis. 2016 Apr;37(7-8):1039-47. doi: 10.1002/elps.201500534. Epub 2016 Feb 25. PubMed PMID: 26826549.

2: Ryan RO. Metabolic annotation of 2-ethylhydracrylic acid. Clin Chim Acta. 2015 Aug 25;448:91-7. doi: 10.1016/j.cca.2015.06.012. Epub 2015 Jun 23. Review. PubMed PMID: 26115894; PubMed Central PMCID: PMC4618168.

3: Jeong JS, Sim HJ, Lee YM, Yoon HR, Kwon HJ, Hong SP. Chromatographic diagnosis of maple syrup urine disease by measuring the L-alloisoleucine/L-phenylalanine ratio in dried blood spots. J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Jul 15;879(22):2171-4. doi: 10.1016/j.jchromb.2011.05.023. Epub 2011 May 23. PubMed PMID: 21680261.

4: Kato S, Oba Y, Ojika M, Inouye S. Stereoselective incorporation of isoleucine into Cypridina luciferin in Cypridina hilgendorfii (Vargula hilgendorfii). Biosci Biotechnol Biochem. 2006 Jun;70(6):1528-32. PubMed PMID: 16794342.

5: Bodner-Leidecker A, Wendel U, Saudubray JM, Schadewaldt P. Branched-chain L-amino acid metabolism in classical maple syrup urine disease after orthotopic liver transplantation. J Inherit Metab Dis. 2000 Dec;23(8):805-18. PubMed PMID: 11196106.

6: Schadewaldt P, Bodner-Leidecker A, Hammen HW, Wendel U. Formation of L-alloisoleucine in vivo: an L-[13C]isoleucine study in man. Pediatr Res. 2000 Feb;47(2):271-7. PubMed PMID: 10674358.

7: Schadewaldt P, Bodner-Leidecker A, Hammen HW, Wendel U. Significance of L-alloisoleucine in plasma for diagnosis of maple syrup urine disease. Clin Chem. 1999 Oct;45(10):1734-40. PubMed PMID: 10508118.

8: Mamer OA, Lépine FL. 15N conservation in the metabolic conversion of isoleucine to alloisoleucine in the rat. J Mass Spectrom. 1996 Dec;31(12):1382-8. PubMed PMID: 8990521.

9: Schadewaldt P, Wendel U, Hammen HW. Determination of R- and S-3-methyl-2-oxopentanoate enantiomers in human plasma: suitable method for label enrichment analysis. J Chromatogr B Biomed Appl. 1996 Jul 12;682(2):209-18. PubMed PMID: 8844412.

10: Hall TR, Wallin R, Reinhart GD, Hutson SM. Branched chain aminotransferase isoenzymes. Purification and characterization of the rat brain isoenzyme. J Biol Chem. 1993 Feb 15;268(5):3092-8. PubMed PMID: 8381418.

11: Gibson KM, Lee CF, Bennett MJ, Holmes B, Nyhan WL. Combined malonic, methylmalonic and ethylmalonic acid semialdehyde dehydrogenase deficiencies: an inborn error of beta-alanine, L-valine and L-alloisoleucine metabolism? J Inherit Metab Dis. 1993;16(3):563-7. PubMed PMID: 7609453.

12: Mamer OA, Reimer ML. On the mechanisms of the formation of L-alloisoleucine and the 2-hydroxy-3-methylvaleric acid stereoisomers from L-isoleucine in maple syrup urine disease patients and in normal humans. J Biol Chem. 1992 Nov 5;267(31):22141-7. PubMed PMID: 1429566.

13: Wendel U, Even G, Langenbeck U, Schadewaldt P, Hummel W. Determination of (S)- and (R)-2-oxo-3-methylvaleric acid in plasma of patients with maple syrup urine disease. Clin Chim Acta. 1992 Jun 15;208(1-2):85-91. PubMed PMID: 1638756.

14: Schadewaldt P, Dalle-Feste C, Langenbeck U, Wendel U. Oral L-alloisoleucine loading studies in healthy subjects and in patients with maple syrup urine disease. Pediatr Res. 1991 Nov;30(5):430-4. PubMed PMID: 1754297.

15: Schadewaldt P, Hammen HW, Dalle-Feste C, Wendel U. On the mechanism of L-alloisoleucine formation: studies on a healthy subject and in fibroblasts from normals and patients with maple syrup urine disease. J Inherit Metab Dis. 1990;13(2):137-50. PubMed PMID: 2116545.

16: Schadewaldt P, Radeck W, Hammen HW, Staib W. Transamination and oxidative decarboxylation of L-isoleucine, L-alloisoleucine and related 2-oxo acids in perfused rat hind limb muscle. Biochim Biophys Acta. 1989 Jul 21;992(1):115-23. PubMed PMID: 2568853.

17: Downey RS, Karl IE, Bier DM. Branched-chain amino acid interactions in skeletal muscle: isoleucine and L-alloisoleucine. JPEN J Parenter Enteral Nutr. 1986 Sep-Oct;10(5):456-62. PubMed PMID: 3093701.

18: Matthews DE, Ben-Galim E, Haymond MW, Bier DM. Alloisoleucine formation in maple syrup urine disease: isotopic evidence for the mechanism. Pediatr Res. 1980 Jul;14(7):854-7. PubMed PMID: 7413299.

19: Yajima T, Mason K, Katz E. Biogenetic origin of the D-isoleucine and N-methyl-L-alloisoleucine residues in the actinomycins. Antimicrob Agents Chemother. 1976 Feb;9(2):224-32. PubMed PMID: 57739; PubMed Central PMCID: PMC429508.

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L-allo-Isoleucine featured