CCT251545
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MedKoo CAT#: 206578

CAS#: 1661839-45-7

Description: CCT251545 is a potent small-molecule inhibitor of WNT signaling with good oral pharmacokinetics. CCT251545 is a potent and selective chemical probe for the human Mediator complex-associated protein kinases CDK8 and CDK19 with >100-fold selectivity over 291 other kinases. In contrast to type II inhibitors of CDK8 and CDK19, CCT251545 displays potent cell-based activity. CCT251545 alters WNT pathway-regulated gene expression and other on-target effects of modulating CDK8 and CDK19, including expression of genes regulated by STAT1.


Chemical Structure

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CCT251545
CAS# 1661839-45-7

Theoretical Analysis

MedKoo Cat#: 206578
Name: CCT251545
CAS#: 1661839-45-7
Chemical Formula: C23H24ClN5O
Exact Mass: 421.17
Molecular Weight: 421.930
Elemental Analysis: C, 65.47; H, 5.73; Cl, 8.40; N, 16.60; O, 3.79

Price and Availability

Size Price Availability Quantity
5mg USD 350 2 Weeks
10mg USD 550 2 Weeks
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Synonym: CCT251545; CCT 251545; CCT-251545; CAS#1661839-45-7

IUPAC/Chemical Name: 8-(3-chloro-5-(4-(1-methyl-1H-pyrazol-4-yl)phenyl)pyridin-4-yl)-2,8-diazaspiro[4.5]decan-1-one

InChi Key: LBFYQISQYCGDDW-UHFFFAOYSA-N

InChi Code: InChI=1S/C23H24ClN5O/c1-28-15-18(12-27-28)16-2-4-17(5-3-16)19-13-25-14-20(24)21(19)29-10-7-23(8-11-29)6-9-26-22(23)30/h2-5,12-15H,6-11H2,1H3,(H,26,30)

SMILES Code: O=C1NCCC12CCN(C3=C(Cl)C=NC=C3C4=CC=C(C5=CN(C)N=C5)C=C4)CC2

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, not in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 421.93 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Dale T, Clarke PA, Esdar C, Waalboer D, Adeniji-Popoola O, Ortiz-Ruiz MJ,
Mallinger A, Samant RS, Czodrowski P, Musil D, Schwarz D, Schneider K, Stubbs M,
Ewan K, Fraser E, TePoele R, Court W, Box G, Valenti M, de Haven Brandon A, Gowan
S, Rohdich F, Raynaud F, Schneider R, Poeschke O, Blaukat A, Workman P, Schiemann
K, Eccles SA, Wienke D, Blagg J. A selective chemical probe for exploring the
role of CDK8 and CDK19 in human disease. Nat Chem Biol. 2015 Dec;11(12):973-80.
doi: 10.1038/nchembio.1952. Epub 2015 Oct 26. PubMed PMID: 26502155; PubMed
Central PMCID: PMC4677459.


2: Mallinger A, Crumpler S, Pichowicz M, Waalboer D, Stubbs M, Adeniji-Popoola O,
Wood B, Smith E, Thai C, Henley AT, Georgi K, Court W, Hobbs S, Box G, Ortiz-Ruiz
MJ, Valenti M, De Haven Brandon A, TePoele R, Leuthner B, Workman P, Aherne W,
Poeschke O, Dale T, Wienke D, Esdar C, Rohdich F, Raynaud F, Clarke PA, Eccles
SA, Stieber F, Schiemann K, Blagg J. Discovery of potent, orally bioavailable,
small-molecule inhibitors of WNT signaling from a cell-based pathway screen. J
Med Chem. 2015 Feb 26;58(4):1717-35. doi: 10.1021/jm501436m. Epub 2015 Feb 13.
PubMed PMID: 25680029.