WARNING: This product is for research use only, not for human or veterinary use.
MedKoo CAT#: 522541
CAS#: 924636-93-1
Description: AF3442 is a potent and selective mPGES-1 inhibitor (microsomal prostaglandin (PG) E synthase-1). AF3442 caused a concentration-dependent inhibition of PGE(2) in human recombinant mPGES-1 with an IC(50) of 0.06microM. AF3442 is a selective mPGES-1 inhibitor which reduced monocyte PGE(2) generation also in the presence of plasma proteins. Pharmacological inhibition of mPGES-1 did not translate into redirection of PGH(2) metabolism towards other terminal PG synthases in monocytes. The functional relevance of this observation deserves to be investigated in vivo.
MedKoo Cat#: 522541
Name: AF3442
CAS#: 924636-93-1
Chemical Formula: C22H17F3N2O
Exact Mass: 382.1293
Molecular Weight: 382.39
Elemental Analysis: C, 69.10; H, 4.48; F, 14.91; N, 7.33; O, 4.18
Synonym: AF3442; AF-3442; AF 3442.
IUPAC/Chemical Name: N-(9-ethyl-9H-carbazol-3-yl)-2-(trifluoromethyl) benzamide
InChi Key: GHLDHBQXNPNZEI-UHFFFAOYSA-N
InChi Code: InChI=1S/C22H17F3N2O/c1-2-27-19-10-6-4-7-15(19)17-13-14(11-12-20(17)27)26-21(28)16-8-3-5-9-18(16)22(23,24)25/h3-13H,2H2,1H3,(H,26,28)
SMILES Code: O=C(NC1=CC2=C(C=C1)N(CC)C3=C2C=CC=C3)C4=CC=CC=C4C(F)(F)F
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO, not in water
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
The following data is based on the product molecular weight 382.39 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
1: Bruno A, Di Francesco L, Coletta I, Mangano G, Alisi MA, Polenzani L, Milanese
C, Anzellotti P, Ricciotti E, Dovizio M, Di Francesco A, Tacconelli S, Capone ML,
Patrignani P. Effects of AF3442
[N-(9-ethyl-9H-carbazol-3-yl)-2-(trifluoromethyl)benzamide], a novel inhibitor of
human microsomal prostaglandin E synthase-1, on prostanoid biosynthesis in human
monocytes in vitro. Biochem Pharmacol. 2010 Apr 1;79(7):974-81. doi:
10.1016/j.bcp.2009.11.011. Epub 2009 Nov 27. PubMed PMID: 19925781.
