EAD1

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 407210

CAS#: 1644388-26-0

Description: EAD1 is a potent and selective autophagy inhibitor with antiproliferative activity in lung and pancreatic cancer cells. EAD1 had an IC50 of 5.8 μM in the BxPC3 cells and was approximately 8-fold more potent than CQ and HCQ. EAD1 inhibited autophagy, as judged by the cellular accumulation of the autophagy-related autophagosome proteins LC3-II and p62 and induced apoptosis. The increases in LC3-II levels by the analogues were highly correlated with their growth inhibitory IC50s, suggesting that autophagy blockade is closely linked to inhibition of cell proliferation. EAD1 is a viable lead compound for evaluation of the antitumor activity of autophagy inhibitors in vivo.

Chemical Structure

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EAD1
CAS# 1644388-26-0
Instruction

Theoretical Analysis

MedKoo Cat#: 407210
Name: EAD1
CAS#: 1644388-26-0
Chemical Formula: C24H27Cl2N7
Exact Mass: 483.17
Molecular Weight: 484.429
Elemental Analysis: C, 59.51; H, 5.62; Cl, 14.64; N, 20.24

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: EAD1; EAD-1; EAD 1.

IUPAC/Chemical Name: N1-((1-(4-chlorobenzyl)-1H-1,2,3-triazol-4-yl)methyl)-N2-(2-((7-chloroquinolin-4-yl)amino)ethyl)-N2-methylethane-1,2-diamine

InChi Key: NUQMXKCBSUXVTG-UHFFFAOYSA-N

InChi Code: InChI=1S/C24H27Cl2N7/c1-32(13-11-29-23-8-9-28-24-14-20(26)6-7-22(23)24)12-10-27-15-21-17-33(31-30-21)16-18-2-4-19(25)5-3-18/h2-9,14,17,27H,10-13,15-16H2,1H3,(H,28,29)

SMILES Code: ClC1=CC=C2C(N=CC=C2NCCN(C)CCNCC3=CN(CC4=CC=C(Cl)C=C4)N=N3)=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, not in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 484.43 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Nordstrøm LU, Sironi J, Aranda E, Maisonet J, Perez-Soler R, Wu P, Schwartz EL. Discovery of autophagy inhibitors with antiproliferative activity in lung and pancreatic cancer cells. ACS Med Chem Lett. 2015 Jan 8;6(2):134-9. doi: 10.1021/ml500348p. eCollection 2015 Feb 12. PubMed PMID: 25699157; PubMed Central PMCID: PMC4329578.

2: Kono K, Kuzuya F, Yamamoto T, Endo H. Comparative study of cerebral ventricular dilation and cognitive function in patients with Alzheimer's disease of early versus late onset. J Geriatr Psychiatry Neurol. 1994 Jan-Mar;7(1):39-45. PubMed PMID: 8192829.