Necrostatin-1
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MedKoo CAT#: 522437

CAS#: 4311-88-0

Description: Necrostatin-1 is a potent and selective inhibitor of necroptosis, a mechanism of programmed cell death separate from apoptosis. Necrostatin-1 functions as an allosteric inhibitor of the death domain receptor-associated adaptor kinase RIP (RIP1) in the necroptosis pathway. While affecting this distinct cell death pathway, Necrostatin-1 does not present any perturbation of the Fas/TNFR triggered canonical apoptosis cascade. Necrostatin-1 inhibits RIP1 kinase the key upstream kinase involved in the activation of necroptosis (EC50=180nM).


Price and Availability

Size Price Shipping out time Quantity
25mg USD 150 Same day
50mg USD 250 Same day
100mg USD 450 Same day
200mg USD 750 Same day
500mg USD 1650 Same day
1g USD 2750 Same day
2g USD 3950 Same day
5g USD 5650 Same day
Inquire bulk and customized quantity

Pricing updated 2020-09-28. Prices are subject to change without notice.

Necrostatin-1, purity > 98%, is in stock. The same day shipping after order is received.


Chemical Structure

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Theoretical Analysis

MedKoo Cat#: 522437
Name: Necrostatin-1
CAS#: 4311-88-0
Chemical Formula: C13H13N3OS
Exact Mass: 259.07793
Molecular Weight: 259.33
Elemental Analysis: C, 60.21; H, 5.05; N, 16.20; O, 6.17; S, 12.36


Synonym: Necrostatin-1; Necrostatin1; Necrostatin 1; Nec-1; Nec 1; Nec1

IUPAC/Chemical Name: 5-(1H-indol-3-ylmethyl)-3-methyl-2-thioxo-4-imidazolidinone

InChi Key: TXUWMXQFNYDOEZ-UHFFFAOYSA-N

InChi Code: InChI=1S/C13H13N3OS/c1-16-12(17)11(15-13(16)18)6-8-7-14-10-5-3-2-4-9(8)10/h2-5,7,11,14H,6H2,1H3,(H,15,18)

SMILES Code: O=C(C(CC1=CNC2=C1C=CC=C2)N3)N(C)C3=S


Technical Data

Appearance:
Light yellow solid powder

Purity:
>98% (or refer to the Certificate of Analysis)

Certificate of Analysis:

Safety Data Sheet (SDS):

Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition:
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility:
Soluble in DMSO, not in water

Shelf Life:
>2 years if stored properly

Drug Formulation:
This drug may be formulated in DMSO

Stock Solution Storage:
0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code:
2934.99.9001


References

1: Mattiolo P, Yuste VJ, Boix J, Ribas J. Autophagy exacerbates caspase-dependent apoptotic cell death after short times of starvation. Biochem Pharmacol. 2015 Oct 3. pii: S0006-2952(15)00631-0. doi: 10.1016/j.bcp.2015.09.021. [Epub ahead of print] PubMed PMID: 26441250.

2: Suzuki T, Kikuguchi C, Sharma S, Sasaki T, Tokumasu M, Adachi S, Natsume T, Kanegae Y, Yamamoto T. CNOT3 suppression promotes necroptosis by stabilizing mRNAs for cell death-inducing proteins. Sci Rep. 2015 Oct 6;5:14779. doi: 10.1038/srep14779. PubMed PMID: 26437789.

3: Zhou C, Chen Z, Lu X, Wu H, Yang Q, Xu D. Icaritin activates JNK-dependent mPTP necrosis pathway in colorectal cancer cells. Tumour Biol. 2015 Oct 1. [Epub ahead of print] PubMed PMID: 26427664.

4: Paredes-Juarez GA, Sahasrabudhe NM, Tjoelker RS, de Haan BJ, Engelse MA, de Koning EJ, Faas MM, de Vos P. DAMP production by human islets under low oxygen and nutrients in the presence or absence of an immunoisolating-capsule and necrostatin-1. Sci Rep. 2015 Sep 30;5:14623. doi: 10.1038/srep14623. PubMed PMID: 26419792.

5: Kharaziha P, Chioureas D, Baltatzis G, Fonseca P, Rodriguez P, Gogvadze V, Lennartsson L, Björklund AC, Zhivotovsky B, Grandér D, Egevad L, Nilsson S, Panaretakis T. Sorafenib-induced defective autophagy promotes cell death by necroptosis. Oncotarget. 2015 Sep 22. [Epub ahead of print] PubMed PMID: 26416459.

6: Zhu L, Zhang M, Liu Y, Wang Y, Ma X. Necrostatin-1 Protects Against Ischemia/Reperfusion Injury by Inhibition of RIP-1 (Receptor-Interacting Protein 1) in a Rat Flap Model. Plast Reconstr Surg. 2015 Oct;136(4 Suppl):33. doi: 10.1097/01.prs.0000472315.28383.8f. PubMed PMID: 26397525.

7: Wang JS, Wu D, Huang DY, Lin WW. TAK1 inhibition-induced RIP1-dependent apoptosis in murine macrophages relies on constitutive TNF-α signaling and ROS production. J Biomed Sci. 2015 Sep 18;22(1):76. doi: 10.1186/s12929-015-0182-7. PubMed PMID: 26381601; PubMed Central PMCID: PMC4574455.

8: Reddy AS, Abraham A, McClain SA, Clark RA, Ralen P, Sandoval S, Singer AJ. The Role of Necroptosis in Burn Injury Progression in a Rat Comb Burn Model. Acad Emerg Med. 2015 Oct;22(10):1181-6. doi: 10.1111/acem.12768. Epub 2015 Sep 16. PubMed PMID: 26375585.

9: Wu JR, Wang J, Zhou SK, Yang L, Yin JL, Cao JP, Cheng YB. Necrostatin-1 protection of dopaminergic neurons. Neural Regen Res. 2015 Jul;10(7):1120-4. doi: 10.4103/1673-5374.160108. PubMed PMID: 26330837; PubMed Central PMCID: PMC4541245.

10: Qiu X, Klausen C, Cheng JC, Leung PC. CD40 ligand induces RIP1-dependent, necroptosis-like cell death in low-grade serous but not serous borderline ovarian tumor cells. Cell Death Dis. 2015 Aug 27;6:e1864. doi: 10.1038/cddis.2015.229. PubMed PMID: 26313915; PubMed Central PMCID: PMC4558516.

11: Zou X, Zhang M, Sun Y, Zhao S, Wei Y, Zhang X, Jiang C, Liu H. Inhibitory effects of 3-bromopyruvate in human nasopharyngeal carcinoma cells. Oncol Rep. 2015 Oct;34(4):1895-904. doi: 10.3892/or.2015.4147. Epub 2015 Jul 23. PubMed PMID: 26239511.

12: Ding W, Shang L, Huang JF, Li N, Chen D, Xue LX, Xiong K. Receptor interacting protein 3-induced RGC-5 cell necroptosis following oxygen glucose deprivation. BMC Neurosci. 2015 Aug 4;16:49. doi: 10.1186/s12868-015-0187-x. PubMed PMID: 26238997; PubMed Central PMCID: PMC4524047.

13: Skrzeczyńska-Moncznik J, Bzowska M, Nogieć A, Sroka A, Zarębski M, Vallières L, Guzik K. Rapid externalization of 27-kDa heat shock protein (HSP27) and atypical cell death in neutrophils treated with the sphingolipid analog drug FTY720. J Leukoc Biol. 2015 Oct;98(4):591-9. doi: 10.1189/jlb.3VMA1114-522RR. Epub 2015 Jul 27. PubMed PMID: 26216939.

14: Afonso MB, Rodrigues PM, Carvalho T, Caridade M, Borralho P, Cortez-Pinto H, Castro RE, Rodrigues CM. Necroptosis is a key pathogenic event in human and experimental murine models of non-alcoholic steatohepatitis. Clin Sci (Lond). 2015 Oct 1;129(8):721-39. doi: 10.1042/CS20140732. Epub 2015 Jun 15. PubMed PMID: 26201023.

15: Ikari A, Taga S, Watanabe R, Sato T, Shimobaba S, Sonoki H, Endo S, Matsunaga T, Sakai H, Yamaguchi M, Yamazaki Y, Sugatani J. Clathrin-dependent endocytosis of claudin-2 by DFYSP peptide causes lysosomal damage in lung adenocarcinoma A549 cells. Biochim Biophys Acta. 2015 Oct;1848(10 Pt A):2326-36. doi: 10.1016/j.bbamem.2015.07.003. Epub 2015 Jul 8. PubMed PMID: 26163137.

16: Yelamanchili SV, Lamberty BG, Rennard DA, Morsey BM, Hochfelder CG, Meays BM, Levy E, Fox HS. MiR-21 in Extracellular Vesicles Leads to Neurotoxicity via TLR7 Signaling in SIV Neurological Disease. PLoS Pathog. 2015 Jul 8;11(7):e1005032. doi: 10.1371/journal.ppat.1005032. eCollection 2015 Jul. PubMed PMID: 26154133; PubMed Central PMCID: PMC4496044.

17: Miki Y, Akimoto J, Moritake K, Hironaka C, Fujiwara Y. Photodynamic therapy using talaporfin sodium induces concentration-dependent programmed necroptosis in human glioblastoma T98G cells. Lasers Med Sci. 2015 Aug;30(6):1739-45. doi: 10.1007/s10103-015-1783-9. Epub 2015 Jun 25. PubMed PMID: 26109138.

18: Opel D, Schnaiter A, Dodier D, Jovanovic M, Gerhardinger A, Idler I, Mertens D, Bullinger L, Stilgenbauer S, Fulda S. Targeting inhibitor of apoptosis proteins by Smac mimetic elicits cell death in poor prognostic subgroups of chronic lymphocytic leukemia. Int J Cancer. 2015 Dec 15;137(12):2959-70. doi: 10.1002/ijc.29650. Epub 2015 Jul 31. PubMed PMID: 26096065.

19: Steinwascher S, Nugues AL, Schoeneberger H, Fulda S. Identification of a novel synergistic induction of cell death by Smac mimetic and HDAC inhibitors in acute myeloid leukemia cells. Cancer Lett. 2015 Sep 28;366(1):32-43. doi: 10.1016/j.canlet.2015.05.020. Epub 2015 May 28. PubMed PMID: 26028172.

20: Rosentreter D, Funken D, Reifart J, Mende K, Rentsch M, Khandoga A. RIP1-Dependent Programmed Necrosis is Negatively Regulated by Caspases During Hepatic Ischemia-Reperfusion. Shock. 2015 Jul;44(1):72-6. doi: 10.1097/SHK.0000000000000371. PubMed PMID: 26009812.