WARNING: This product is for research use only, not for human or veterinary use.
MedKoo CAT#: 522382
Description: kb NB 142-70 is a selective protein kinase D (PKD) inhibitor with IC50 values are 28.3, 58.7 and 53.2 nM for PKD1, 2 and 3 respectively. kb NB 142-70 prevents wound-induced migration of intestinal epithelial cells. PKD1, the most studied member of the PKD family, is implicated in the regulation of a remarkable array of fundamental biological processes, including signal transduction, cell proliferation and differentiation, membrane trafficking, hormone secretion, immune regulation, cardiac hypertrophy, angiogenesis, and cancer.
MedKoo Cat#: 522382
Name: kb NB 142-70
Chemical Formula: C11H9NO2S2
Exact Mass: 251.00747
Molecular Weight: 251.32
Elemental Analysis: C, 52.57; H, 3.61; N, 5.57; O, 12.73; S, 25.51
Synonym: kb NB 142-70; kb NB142-70; kb NB-142-70.
IUPAC/Chemical Name: 9-hydroxy-3,4-dihydrobenzo[4,5]thieno[2,3-f][1,4]thiazepin-5(2H)-one
InChi Key: DHUAGGSHTKPOHU-UHFFFAOYSA-N
InChi Code: InChI=1S/C11H9NO2S2/c13-6-1-2-8-7(5-6)9-10(16-8)11(14)12-3-4-15-9/h1-2,5,13H,3-4H2,(H,12,14)
SMILES Code: O=C1NCCSC2=C1SC3=CC=C(O)C=C32
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO, not in water
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
The following data is based on the product molecular weight 251.32 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.15 mL||5.76 mL||11.51 mL|
|5 mM||0.23 mL||1.15 mL||2.3 mL|
|10 mM||0.12 mL||0.58 mL||1.15 mL|
|50 mM||0.02 mL||0.12 mL||0.23 mL|
1: Ni Y, Wang L, Zhang J, Pang Z, Liu Q, Du J. PKD1 is downregulated in non-small cell lung cancer and mediates the feedback inhibition of mTORC1-S6K1 axis in response to phorbol ester. Int J Biochem Cell Biol. 2015 Mar;60:34-42. doi: 10.1016/j.biocel.2014.12.018. Epub 2015 Jan 9. PubMed PMID: 25578563.
2: Sinnett-Smith J, Ni Y, Wang J, Ming M, Young SH, Rozengurt E. Protein kinase D1 mediates class IIa histone deacetylase phosphorylation and nuclear extrusion in intestinal epithelial cells: role in mitogenic signaling. Am J Physiol Cell Physiol. 2014 May 15;306(10):C961-71. doi: 10.1152/ajpcell.00048.2014. Epub 2014 Mar 19. PubMed PMID: 24647541; PubMed Central PMCID: PMC4024715.
3: Ni Y, Sinnett-Smith J, Young SH, Rozengurt E. PKD1 mediates negative feedback of PI3K/Akt activation in response to G protein-coupled receptors. PLoS One. 2013 Sep 9;8(9):e73149. doi: 10.1371/journal.pone.0073149. eCollection 2013. Erratum in: PLoS One. 2014;9(1). doi:10.1371/annotation/70d3c7b0-9718-4bb1-abed-d0defc3b7fc4. PubMed PMID: 24039875; PubMed Central PMCID: PMC3767810.
4: Young SH, Rozengurt N, Sinnett-Smith J, Rozengurt E. Rapid protein kinase D1 signaling promotes migration of intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2012 Aug 1;303(3):G356-66. doi: 10.1152/ajpgi.00025.2012. Epub 2012 May 17. PubMed PMID: 22595992; PubMed Central PMCID: PMC3423107.