Asiatic Acid
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MedKoo CAT#: 601122

CAS#: 464-92-6

Description: Asiatic acid is a pentacyclic triterpene isolated from a variety of plants, including C. asiatica, used in traditional medicines. In addition to anti-inflammatory actions, asiatic acid stimulates wound healing by increasing collagen production. It has been found to induce cell cycle arrest and apoptosis in breast cancer cells and block angiogenesis in cells and tumors from glioblastomas. It also reduces neuronal damage and cognitive defects resulting from glutamate administration in vivo in mice while demonstrating protective effects against glutamate-induced apoptosis in isolated SH-SY5Y cells.

Chemical Structure

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Asiatic Acid
CAS# 464-92-6
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 601122
Name: Asiatic Acid
CAS#: 464-92-6
Chemical Formula: C30H48O5
Exact Mass: 488.35
Molecular Weight: 488.700
Elemental Analysis: C, 73.73; H, 9.90; O, 16.37

Price and Availability

Size Price Availability Quantity
50mg USD 220 2 Weeks
100mg USD 360 2 Weeks
500mg USD 615 2 Weeks
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Synonym: Dammarolic Acid; NSC 166063; NSC166063; NSC166063

IUPAC/Chemical Name: (4α)-​2α,​3β,​23-​trihydroxy-​urs-​12-​en-​28-​oic acid

InChi Key: JXSVIVRDWWRQRT-UYDOISQJSA-N

InChi Code: InChI=1S/C30H48O5/c1-17-9-12-30(25(34)35)14-13-28(5)19(23(30)18(17)2)7-8-22-26(3)15-20(32)24(33)27(4,16-31)21(26)10-11-29(22,28)6/h7,17-18,20-24,31-33H,8-16H2,1-6H3,(H,34,35)/t17-,18+,20-,21-,22-,23+,24+,26+,27+,28-,29-,30+/m1/s1

SMILES Code: C[C@@]1(CO)[C@@H](O)[C@H](O)C[C@]2(C)[C@@]3([H])CC=C4[C@]5([H])[C@@H](C)[C@H](C)CC[C@@](C(O)=O)5CC[C@](C)4[C@@](C)3CC[C@@]12[H]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO.

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Instruction:
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Biological target: Asiatic acid, a pentacyclic triterpene found in Centella asiatica, induces apoptosis in melanoma cells.
In vitro activity: This study began by assessing the impact of AA (Asiatic Acid) on tongue cancer cell viability via an MTT assay, which revealed that AA application significantly suppressed the viability of these cells with an IC50 value of approximately 40°μM (Figure 1A). Consistent with this, AA treatment (40°μM) significantly suppressed the colony forming activity of Tca8113 cells (Figure 1B). Reference: Front Pharmacol. 2021; 12: 690612. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187906/
In vivo activity: AngII infusion rats showed a significant increase in the ratio of weight/tibia length (HW/TL), and this increase was attenuated in AA (Asiatic Acid)-treated rats (Fig. 2a). The thickness of the left ventricular post wall at the end-diastole (LVPWd) and the end-systole (LVPWs) was higher in AngII infusion rats, while AA treatment attenuated these alterations (Fig. 2b). Results of WGA staining showed that the cell size of cardiomyocytes was significantly increased in the myocardium of the Ang-II infusion animal model, which was significantly attenuated in AA-treated rats (Fig. 2c). To determine the effect of AA on cardiac fibrosis, heart sections were stained with Masson’s staining. In Fig. 2d, interstitial fibrosis is demonstrated by the blue areas. Quantitative data revealed increased collagen deposition in AngII-induced rats, while was significantly attenuated in AA-treated rats (Fig. 2d). Meanwhile, significant increase of ANP and β-MHC protein expression was observed in the hypertrophic rat myocardium, while their expression was inhibited in AA-treated rat (Fig. 2e). Collectively, AA treatment can inhibit AngII-induced cardiac hypertrophy and fibrosis. Reference: Nutr Metab (Lond). 2021; 18: 71. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278598/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 59.8 122.43
DMSO:PBS (pH 7.2) (1:3) 0.3 0.51
DMF 20.0 40.92
Ethanol 10.0 20.46

Preparing Stock Solutions

The following data is based on the product molecular weight 488.70 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Yang Z, Feng L, Huang J, Zhang X, Lin W, Wang B, Cui L, Lin S, Li G. Asiatic acid protects articular cartilage through promoting chondrogenesis and inhibiting inflammation and hypertrophy in osteoarthritis. Eur J Pharmacol. 2021 Jun 24;907:174265. doi: 10.1016/j.ejphar.2021.174265. Epub ahead of print. PMID: 34174266. 2. Li J, Chen K, Huang J, Chu D, Tian M, Huang K, Ma C. Asiatic Acid Induces Endoplasmic Reticulum Stress and Activates the Grp78/IRE1α/JNK and Calpain Pathways to Inhibit Tongue Cancer Growth. Front Pharmacol. 2021 May 26;12:690612. doi: 10.3389/fphar.2021.690612. PMID: 34122116; PMCID: PMC8187906. 3. Li H, Tian X, Ruan Y, Xing J, Meng Z. Asiatic acid alleviates Ang-II induced cardiac hypertrophy and fibrosis via miR-126/PIK3R2 signaling. Nutr Metab (Lond). 2021 Jul 13;18(1):71. doi: 10.1186/s12986-021-00596-7. PMID: 34256802; PMCID: PMC8278598. 4. Fang M, Wan W, Li Q, Wan W, Long Y, Liu H, Yang X. Asiatic acid attenuates diabetic retinopathy through TLR4/MyD88/NF-κB p65 mediated modulation of microglia polarization. Life Sci. 2021 Jul 15;277:119567. doi: 10.1016/j.lfs.2021.119567. Epub 2021 May 6. PMID: 33965378.
In vitro protocol: 1. Yang Z, Feng L, Huang J, Zhang X, Lin W, Wang B, Cui L, Lin S, Li G. Asiatic acid protects articular cartilage through promoting chondrogenesis and inhibiting inflammation and hypertrophy in osteoarthritis. Eur J Pharmacol. 2021 Jun 24;907:174265. doi: 10.1016/j.ejphar.2021.174265. Epub ahead of print. PMID: 34174266. 2. Li J, Chen K, Huang J, Chu D, Tian M, Huang K, Ma C. Asiatic Acid Induces Endoplasmic Reticulum Stress and Activates the Grp78/IRE1α/JNK and Calpain Pathways to Inhibit Tongue Cancer Growth. Front Pharmacol. 2021 May 26;12:690612. doi: 10.3389/fphar.2021.690612. PMID: 34122116; PMCID: PMC8187906.
In vivo protocol: 1. Li H, Tian X, Ruan Y, Xing J, Meng Z. Asiatic acid alleviates Ang-II induced cardiac hypertrophy and fibrosis via miR-126/PIK3R2 signaling. Nutr Metab (Lond). 2021 Jul 13;18(1):71. doi: 10.1186/s12986-021-00596-7. PMID: 34256802; PMCID: PMC8278598. 2. Fang M, Wan W, Li Q, Wan W, Long Y, Liu H, Yang X. Asiatic acid attenuates diabetic retinopathy through TLR4/MyD88/NF-κB p65 mediated modulation of microglia polarization. Life Sci. 2021 Jul 15;277:119567. doi: 10.1016/j.lfs.2021.119567. Epub 2021 May 6. PMID: 33965378.

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1: Park JH, Seo YH, Jang JH, Jeong CH, Lee S, Park B. Asiatic acid attenuates methamphetamine-induced neuroinflammation and neurotoxicity through blocking of NF-kB/STAT3/ERK and mitochondria-mediated apoptosis pathway. J Neuroinflammation. 2017 Dec 11;14(1):240. doi: 10.1186/s12974-017-1009-0. PMID: 29228978; PMCID: PMC5725763.


2: Fang M, Wan W, Li Q, Wan W, Long Y, Liu H, Yang X. Asiatic acid attenuates diabetic retinopathy through TLR4/MyD88/NF-κB p65 mediated modulation of microglia polarization. Life Sci. 2021 Jul 15;277:119567. doi: 10.1016/j.lfs.2021.119567. Epub 2021 May 6. PMID: 33965378.


3: Zhu Z, Cui L, Yang J, Vong CT, Hu Y, Xiao J, Chan G, He Z, Zhong Z. Anticancer effects of asiatic acid against doxorubicin-resistant breast cancer cells via an AMPK-dependent pathway in vitro. Phytomedicine. 2021 Nov;92:153737. doi: 10.1016/j.phymed.2021.153737. Epub 2021 Sep 21. PMID: 34560519.


4: Ding L, Liu T, Ma J. Neuroprotective mechanisms of Asiatic acid. Heliyon. 2023 Apr 26;9(5):e15853. doi: 10.1016/j.heliyon.2023.e15853. PMID: 37180926; PMCID: PMC10172897.


5: Yi C, Song M, Sun L, Si L, Yu D, Li B, Lu P, Wang W, Wang X. Asiatic Acid Alleviates Myocardial Ischemia-Reperfusion Injury by Inhibiting the ROS-Mediated Mitochondria-Dependent Apoptosis Pathway. Oxid Med Cell Longev. 2022 Feb 14;2022:3267450. doi: 10.1155/2022/3267450. PMID: 35198095; PMCID: PMC8860531.


6: Qiu F, Yuan Y, Luo W, Gong YS, Zhang ZM, Liu ZM, Gao L. Asiatic acid alleviates ischemic myocardial injury in mice by modulating mitophagy- and glycophagy-based energy metabolism. Acta Pharmacol Sin. 2022 Jun;43(6):1395-1407. doi: 10.1038/s41401-021-00763-9. Epub 2021 Sep 14. PMID: 34522006; PMCID: PMC9160258.


7: Tian M, Chen K, Huang J, Chu D, Li J, Huang K, Ma C. Asiatic acid inhibits angiogenesis and vascular permeability through the VEGF/VEGFR2 signaling pathway to inhibit the growth and metastasis of breast cancer in mice. Phytother Res. 2021 Nov;35(11):6389-6400. doi: 10.1002/ptr.7292. Epub 2021 Sep 19. PMID: 34541711.


8: Bylka W, Znajdek-Awiżeń P, Studzińska-Sroka E, Dańczak-Pazdrowska A, Brzezińska M. Centella asiatica in dermatology: an overview. Phytother Res. 2014 Aug;28(8):1117-24. doi: 10.1002/ptr.5110. Epub 2014 Jan 7. PMID: 24399761.


9: Qiu W, Zhang X, Pang X, Huang J, Zhou S, Wang R, Tang Z, Su R. Asiatic acid alleviates LPS-induced acute kidney injury in broilers by inhibiting oxidative stress and ferroptosis via activation of the Nrf2 pathway. Food Chem Toxicol. 2022 Dec;170:113468. doi: 10.1016/j.fct.2022.113468. Epub 2022 Oct 14. PMID: 36244460.


10: Chen X, Han D, Liu T, Huang C, Hu Z, Tan X, Wu S. Asiatic acid improves high-fat-diet-induced osteoporosis in mice via regulating SIRT1/FOXO1 signaling and inhibiting oxidative stress. Histol Histopathol. 2022 Aug;37(8):769-777. doi: 10.14670/HH-18-446. Epub 2022 Mar 1. PMID: 35229281.


11: Kukula O, Kırmızıkan S, Tiryaki ES, Çiçekli MN, Günaydın C. Asiatic acid exerts an anti-psoriatic effect in the imiquimod-induced psoriasis model in mice. Immunopharmacol Immunotoxicol. 2022 Jun;44(3):367-372. doi: 10.1080/08923973.2022.2048849. Epub 2022 Mar 7. PMID: 35253588.


12: Sycz Z, Tichaczek-Goska D, Wojnicz D. Anti-Planktonic and Anti-Biofilm Properties of Pentacyclic Triterpenes-Asiatic Acid and Ursolic Acid as Promising Antibacterial Future Pharmaceuticals. Biomolecules. 2022 Jan 7;12(1):98. doi: 10.3390/biom12010098. PMID: 35053246; PMCID: PMC8774094.


13: Islam MT, Ali ES, Uddin SJ, Khan IN, Shill MC, de Castro E Sousa JM, de Alencar MVOB, Melo-Cavalcante AAC, Mubarak MS. Anti-Cancer Effects of Asiatic Acid, a Triterpene from Centilla asiatica L: A Review. Anticancer Agents Med Chem. 2020;20(5):536-547. doi: 10.2174/1871520619666191211103006. PMID: 31823705.


14: Niu K, Bai P, Yang B, Feng X, Qiu F. Asiatic acid alleviates metabolism disorders in ob/ob mice: mechanistic insights. Food Funct. 2022 Jul 4;13(13):6934-6946. doi: 10.1039/d2fo01069k. PMID: 35696250.


15: Lv J, Sharma A, Zhang T, Wu Y, Ding X. Pharmacological Review on Asiatic Acid and Its Derivatives: A Potential Compound. SLAS Technol. 2018 Apr;23(2):111-127. doi: 10.1177/2472630317751840. Epub 2018 Jan 23. PMID: 29361877.


16: Li Y, He XL, Zhou LP, Huang XZ, Li S, Guan S, Li J, Zhang L. Asiatic acid alleviates liver fibrosis via multiple signaling pathways based on integrated network pharmacology and lipidomics. Eur J Pharmacol. 2022 Sep 15;931:175193. doi: 10.1016/j.ejphar.2022.175193. Epub 2022 Aug 10. PMID: 35963324.


17: Hong G, Zhou L, Han X, Sun P, Chen Z, He W, Tickner J, Chen L, Shi X, Xu J. Asiatic Acid Inhibits OVX-Induced Osteoporosis and Osteoclastogenesis Via Regulating RANKL-Mediated NF-κb and Nfatc1 Signaling Pathways. Front Pharmacol. 2020 Mar 27;11:331. doi: 10.3389/fphar.2020.00331. PMID: 32292339; PMCID: PMC7120530.


18: Liu D, Qian T, Li P, Li W, Sun S, Jiang JJ. Asiatic Acid Improves Extracellular Matrix Remodeling in Vocal Fold Scarring Via SMAD7 Activation. Laryngoscope. 2022 Jun;132(6):1237-1244. doi: 10.1002/lary.29884. Epub 2021 Sep 30. PMID: 34591990.


19: Hu X, Li B, Li L, Li B, Luo J, Shen B. Asiatic Acid Protects against Doxorubicin-Induced Cardiotoxicity in Mice. Oxid Med Cell Longev. 2020 May 15;2020:5347204. doi: 10.1155/2020/5347204. PMID: 32509145; PMCID: PMC7246415.


20: Zou W, Zhang J, Zhang K, Peng Z, Xin R, Wang L, Li J. Asiatic Acid Attenuates Inflammation Induced by Salmonella via Upregulating LncRNA TVX1 in Microglia. Int J Mol Sci. 2022 Sep 19;23(18):10978. doi: 10.3390/ijms231810978. PMID: 36142890; PMCID: PMC9504590.