JSH-23
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MedKoo CAT#: 406426

CAS#: 749886-87-1

Description: JSH-23 is a nuclear factor-kappa B (NF-κB) nuclear translocation inhibitor. JSH-23 inhibits LPS and cytokine-induced nuclear translocation of the p65 subunit of NF-kB as analyzed by EMSA and western blot. JSH-23 treatment significantly reversed the nerve conduction and nerve blood flow deficits seen in diabetic animals. Reduction in mechanical pain threshold was also partially corrected by the treatment. Protein expression studies showed that nuclear translocation of p65/p50 subunit was inhibited by JSH-23 treatment in the sciatic nerve. The treatment also lowered the elevated IL-6, TNF-α, cyclo-oxygenase (COX-2) and inducible nitric oxide synthase (iNOS) levels/expression, indicating reduction in the inflammatory damage of the sciatic nerve. Apart from these effects, JSH-23 also increased Nrf2 and hemeoxygenase-1 (HO-1) levels which could imply its potential in increasing the strength of antioxidant defence.

Chemical Structure

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JSH-23
CAS# 749886-87-1
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 406426
Name: JSH-23
CAS#: 749886-87-1
Chemical Formula: C16H20N2
Exact Mass: 240.16
Molecular Weight: 240.343
Elemental Analysis: C, 79.96; H, 8.39; N, 11.66

Price and Availability

Size Price Availability Quantity
5mg USD 90 Ready to ship
10mg USD 150 Ready to ship
25mg USD 250 Ready to ship
50mg USD 400 Ready to ship
100mg USD 700 Ready to ship
200mg USD 1250 Ready to ship
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Synonym: JSH23; JSH 23; JSH-23.

IUPAC/Chemical Name: 4-methyl-N1-(3-phenylpropyl)benzene-1,2-diamine

InChi Key: YMFNPBSZFWXMAD-UHFFFAOYSA-N

InChi Code: InChI=1S/C16H20N2/c1-13-9-10-16(15(17)12-13)18-11-5-8-14-6-3-2-4-7-14/h2-4,6-7,9-10,12,18H,5,8,11,17H2,1H3

SMILES Code: NC1=CC(C)=CC=C1NCCCC2=CC=CC=C2

Appearance: white solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, not in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:         

Biological target: JSH-23 inhibits NF-κB transcriptional activity with an IC50 of 7.1 μM in RAW 264.7 cells.
In vitro activity: JSH‑23, an inhibitor of the NF‑κB pathway, impaired the migration of liver cancer cells, and was found to act synergistically with cordycepin. Furthermore, cordycepin treatment reduced the chemotactic migration ability of liver cancer cells to stromal cell‑derived factor 1 (SDF1), which was significantly enhanced following treatment with JSH‑23. Reference: Int J Mol Med. 2020 Jan;45(1):141-150. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6889938/
In vivo activity: The antidepressant-like effects of JSH-23 were investigated in the chronic mild stress (CMS) mouse model. Administration of JSH-23 significantly prevented the decreased sucrose preference in the SPT (sucrose preference test) and prevented the increased immobility time in the FST (forced swimming test) caused by CMS, but had no effect on locomotor activity. Expression of NF-κB p65 protein in the hippocampus was decreased, and elevated levels of IL-6 and TNF-α were reduced, after JSH-23 administration. In addition to its anti-inflammatory effect, JSH-23 treatment increased the expression of SOD and Nrf 2 in the hippocampus, suggesting that it strengthens antioxidant defense. Reference: Pharmacol Biochem Behav. 2018 Jun;169:59-66. https://www.sciencedirect.com/science/article/abs/pii/S0091305718300364?via%3Dihub

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 25.0 104.02
DMSO 43.0 178.91
DMSO:PBS (pH 7.2) (1:2) 0.3 1.25
Ethanol 15.0 62.41

Preparing Stock Solutions

The following data is based on the product molecular weight 240.34 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Guo Z, Chen W, Dai G, Huang Y. Cordycepin suppresses the migration and invasion of human liver cancer cells by downregulating the expression of CXCR4. Int J Mol Med. 2020 Jan;45(1):141-150. doi: 10.3892/ijmm.2019.4391. Epub 2019 Oct 31. PMID: 31746344; PMCID: PMC6889938. 2. Kumar A, Negi G, Sharma SS. JSH-23 targets nuclear factor-kappa B and reverses various deficits in experimental diabetic neuropathy: effect on neuroinflammation and antioxidant defence. Diabetes Obes Metab. 2011 Aug;13(8):750-8. doi: 10.1111/j.1463-1326.2011.01402.x. PMID: 21447040. 3. Wang Q, Dong X, Li N, Wang Y, Guan X, Lin Y, Kang J, Zhang X, Zhang Y, Li X, Xu T. JSH-23 prevents depressive-like behaviors in mice subjected to chronic mild stress: Effects on inflammation and antioxidant defense in the hippocampus. Pharmacol Biochem Behav. 2018 Jun;169:59-66. doi: 10.1016/j.pbb.2018.04.005. Epub 2018 Apr 21. PMID: 29684396.
In vitro protocol: 1. Guo Z, Chen W, Dai G, Huang Y. Cordycepin suppresses the migration and invasion of human liver cancer cells by downregulating the expression of CXCR4. Int J Mol Med. 2020 Jan;45(1):141-150. doi: 10.3892/ijmm.2019.4391. Epub 2019 Oct 31. PMID: 31746344; PMCID: PMC6889938.
In vivo protocol: 1. Kumar A, Negi G, Sharma SS. JSH-23 targets nuclear factor-kappa B and reverses various deficits in experimental diabetic neuropathy: effect on neuroinflammation and antioxidant defence. Diabetes Obes Metab. 2011 Aug;13(8):750-8. doi: 10.1111/j.1463-1326.2011.01402.x. PMID: 21447040. 2. Wang Q, Dong X, Li N, Wang Y, Guan X, Lin Y, Kang J, Zhang X, Zhang Y, Li X, Xu T. JSH-23 prevents depressive-like behaviors in mice subjected to chronic mild stress: Effects on inflammation and antioxidant defense in the hippocampus. Pharmacol Biochem Behav. 2018 Jun;169:59-66. doi: 10.1016/j.pbb.2018.04.005. Epub 2018 Apr 21. PMID: 29684396.

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8: Kumar A, Negi G, Sharma SS. JSH-23 targets nuclear factor-kappa B and reverses various deficits in experimental diabetic neuropathy: effect on neuroinflammation and antioxidant defence. Diabetes Obes Metab. 2011 Aug;13(8):750-8. doi: 10.1111/j.1463-1326.2011.01402.x. PubMed PMID: 21447040.

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