WARNING: This product is for research use only, not for human or veterinary use.
MedKoo CAT#: 406426
Description: JSH-23 is a nuclear factor-kappa B (NF-κB) nuclear translocation inhibitor. JSH-23 inhibits LPS and cytokine-induced nuclear translocation of the p65 subunit of NF-kB as analyzed by EMSA and western blot. JSH-23 treatment significantly reversed the nerve conduction and nerve blood flow deficits seen in diabetic animals. Reduction in mechanical pain threshold was also partially corrected by the treatment. Protein expression studies showed that nuclear translocation of p65/p50 subunit was inhibited by JSH-23 treatment in the sciatic nerve. The treatment also lowered the elevated IL-6, TNF-α, cyclo-oxygenase (COX-2) and inducible nitric oxide synthase (iNOS) levels/expression, indicating reduction in the inflammatory damage of the sciatic nerve. Apart from these effects, JSH-23 also increased Nrf2 and hemeoxygenase-1 (HO-1) levels which could imply its potential in increasing the strength of antioxidant defence.
MedKoo Cat#: 406426
Chemical Formula: C16H20N2
Exact Mass: 240.16265
Molecular Weight: 240.3434
Elemental Analysis: C, 79.96; H, 8.39; N, 11.66
Synonym: JSH23; JSH 23; JSH-23.
IUPAC/Chemical Name: 4-methyl-N1-(3-phenylpropyl)benzene-1,2-diamine
InChi Key: YMFNPBSZFWXMAD-UHFFFAOYSA-N
InChi Code: InChI=1S/C16H20N2/c1-13-9-10-16(15(17)12-13)18-11-5-8-14-6-3-2-4-7-14/h2-4,6-7,9-10,12,18H,5,8,11,17H2,1H3
SMILES Code: NC1=CC(C)=CC=C1NCCCC2=CC=CC=C2
Appearance: white solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO, not in water
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
|Biological target:||JSH-23 inhibits NF-κB transcriptional activity with an IC50 of 7.1 μM in RAW 264.7 cells.|
|In vitro activity:||JSH‑23, an inhibitor of the NF‑κB pathway, impaired the migration of liver cancer cells, and was found to act synergistically with cordycepin. Furthermore, cordycepin treatment reduced the chemotactic migration ability of liver cancer cells to stromal cell‑derived factor 1 (SDF1), which was significantly enhanced following treatment with JSH‑23. Reference: Int J Mol Med. 2020 Jan;45(1):141-150. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6889938/|
|In vivo activity:||The antidepressant-like effects of JSH-23 were investigated in the chronic mild stress (CMS) mouse model. Administration of JSH-23 significantly prevented the decreased sucrose preference in the SPT (sucrose preference test) and prevented the increased immobility time in the FST (forced swimming test) caused by CMS, but had no effect on locomotor activity. Expression of NF-κB p65 protein in the hippocampus was decreased, and elevated levels of IL-6 and TNF-α were reduced, after JSH-23 administration. In addition to its anti-inflammatory effect, JSH-23 treatment increased the expression of SOD and Nrf 2 in the hippocampus, suggesting that it strengthens antioxidant defense. Reference: Pharmacol Biochem Behav. 2018 Jun;169:59-66. https://www.sciencedirect.com/science/article/abs/pii/S0091305718300364?via%3Dihub|
|Solvent||Max Conc. mg/mL||Max Conc. mM|
|DMSO:PBS (pH 7.2) (1:2)||0.3||1.25|
The following data is based on the product molecular weight 240.3434 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.15 mL||5.76 mL||11.51 mL|
|5 mM||0.23 mL||1.15 mL||2.3 mL|
|10 mM||0.12 mL||0.58 mL||1.15 mL|
|50 mM||0.02 mL||0.12 mL||0.23 mL|
|Formulation protocol:||1. Guo Z, Chen W, Dai G, Huang Y. Cordycepin suppresses the migration and invasion of human liver cancer cells by downregulating the expression of CXCR4. Int J Mol Med. 2020 Jan;45(1):141-150. doi: 10.3892/ijmm.2019.4391. Epub 2019 Oct 31. PMID: 31746344; PMCID: PMC6889938. 2. Kumar A, Negi G, Sharma SS. JSH-23 targets nuclear factor-kappa B and reverses various deficits in experimental diabetic neuropathy: effect on neuroinflammation and antioxidant defence. Diabetes Obes Metab. 2011 Aug;13(8):750-8. doi: 10.1111/j.1463-1326.2011.01402.x. PMID: 21447040. 3. Wang Q, Dong X, Li N, Wang Y, Guan X, Lin Y, Kang J, Zhang X, Zhang Y, Li X, Xu T. JSH-23 prevents depressive-like behaviors in mice subjected to chronic mild stress: Effects on inflammation and antioxidant defense in the hippocampus. Pharmacol Biochem Behav. 2018 Jun;169:59-66. doi: 10.1016/j.pbb.2018.04.005. Epub 2018 Apr 21. PMID: 29684396.|
|In vitro protocol:||1. Guo Z, Chen W, Dai G, Huang Y. Cordycepin suppresses the migration and invasion of human liver cancer cells by downregulating the expression of CXCR4. Int J Mol Med. 2020 Jan;45(1):141-150. doi: 10.3892/ijmm.2019.4391. Epub 2019 Oct 31. PMID: 31746344; PMCID: PMC6889938.|
|In vivo protocol:||1. Kumar A, Negi G, Sharma SS. JSH-23 targets nuclear factor-kappa B and reverses various deficits in experimental diabetic neuropathy: effect on neuroinflammation and antioxidant defence. Diabetes Obes Metab. 2011 Aug;13(8):750-8. doi: 10.1111/j.1463-1326.2011.01402.x. PMID: 21447040. 2. Wang Q, Dong X, Li N, Wang Y, Guan X, Lin Y, Kang J, Zhang X, Zhang Y, Li X, Xu T. JSH-23 prevents depressive-like behaviors in mice subjected to chronic mild stress: Effects on inflammation and antioxidant defense in the hippocampus. Pharmacol Biochem Behav. 2018 Jun;169:59-66. doi: 10.1016/j.pbb.2018.04.005. Epub 2018 Apr 21. PMID: 29684396.|
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