WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 122472

CAS#: 10578-79-7

Description: N4-Methylcytidine is a nucleoside analogue that inhibits synthesis of human mitochondrial DNA by inhibiting bacterial enzyme uridine-cytidine kinase.

Chemical Structure

CAS# 10578-79-7

Theoretical Analysis

MedKoo Cat#: 122472
Name: N4-Methylcytidine
CAS#: 10578-79-7
Chemical Formula: C10H15N3O5
Exact Mass: 257.10
Molecular Weight: 257.250
Elemental Analysis: C, 46.69; H, 5.88; N, 16.33; O, 31.10

Price and Availability

Size Price Availability Quantity
100mg USD 450 2 Weeks
250mg USD 750 2 Weeks
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Synonym: N4-Methylcytidine; Cytidine, N-methyl-; N(4)-Methylcytidine

IUPAC/Chemical Name: 1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-4-(methylamino)pyrimidin-2(1H)-one


InChi Code: InChI=1S/C10H15N3O5/c1-11-6-2-3-13(10(17)12-6)9-8(16)7(15)5(4-14)18-9/h2-3,5,7-9,14-16H,4H2,1H3,(H,11,12,17)/t5-,7-,8-,9-/m1/s1

SMILES Code: OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=CC(NC)=NC2=O)O1

Appearance: To be determined

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 257.25 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Tieu Ngoc LN, Jung Park S, Thi Huong T, Lee KH, Kang H. N4-methylcytidine
ribosomal RNA methylation in chloroplasts is crucial for chloroplast function,
development, and abscisic acid response in Arabidopsis. J Integr Plant Biol.
2021 Mar;63(3):570-582. doi: 10.1111/jipb.13009. Epub 2020 Sep 30. PMID:

2: Van Haute L, Hendrick AG, D'Souza AR, Powell CA, Rebelo-Guiomar P, Harbour
ME, Ding S, Fearnley IM, Andrews B, Minczuk M. METTL15 introduces
N4-methylcytidine into human mitochondrial 12S rRNA and is required for
mitoribosome biogenesis. Nucleic Acids Res. 2019 Nov 4;47(19):10267-10281. doi:
10.1093/nar/gkz735. PMID: 31665743; PMCID: PMC6821322.

3: Mao S, Sekula B, Ruszkowski M, Ranganathan SV, Haruehanroengra P, Wu Y, Shen
F, Sheng J. Base pairing, structural and functional insights into
N4-methylcytidine (m4C) and N4,N4-dimethylcytidine (m42C) modified RNA. Nucleic
Acids Res. 2020 Oct 9;48(18):10087-10100. doi: 10.1093/nar/gkaa737. PMID:
32941619; PMCID: PMC7544196.

4: Zheng YY, Mao S, Sheng J. Synthesis of N4 -Methylcytidine
(m4 C) and N4 ,N4 -Dimethylcytidine
(m42 C) Modified RNA. Curr Protoc. 2021 Sep;1(9):e248.
doi: 10.1002/cpz1.248. PMID: 34529364.

5: Cheng MY, You XJ, Ding JH, Dai Y, Chen MY, Yuan BF, Feng YQ. Novel dual
methylation of cytidines in the RNA of mammals. Chem Sci. 2021 May
7;12(23):8149-8156. doi: 10.1039/d1sc01972d. PMID: 34194705; PMCID: PMC8208307.

6: Dubin DT, Taylor RH, Davenport LW. Methylation status of 13S ribosomal RNA
from hamster mitochondria: the presence of a novel riboside, N4-methylcytidine.
Nucleic Acids Res. 1978 Nov;5(11):4385-97. doi: 10.1093/nar/5.11.4385. PMID:
724519; PMCID: PMC342756.

7: Suzuki T, Moriyama K, Otsuka C, Loakes D, Negishi K. Template properties of
mutagenic cytosine analogues in reverse transcription. Nucleic Acids Res.
2006;34(22):6438-49. doi: 10.1093/nar/gkl761. Epub 2006 Nov 27. PMID: 17130163;
PMCID: PMC1702492.

8: Biernat J, Ciesiołka J, Górnicki P, Adamiak RW, Kryzosiak WJ, Wiewiórowski M.
New observations concerning the chloroacetaldehyde reaction with some tRNA
constituents. Stable intermediates, kinetics and selectivity of the reaction.
Nucleic Acids Res. 1978 Mar;5(3):789-804. doi: 10.1093/nar/5.3.789. PMID: 25420;
PMCID: PMC342024.