Cyclophosphamide monohydrate

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 100190

CAS#: 6055-19-2 (hydrate)

Description: Cyclophosphamide, also known as cytophosphane among other, is used as chemotherapy and to suppress the immune system. As chemotherapy it is used to treat lymphoma, multiple myeloma, leukemia, ovarian cancer, breast cancer, small cell lung cancer, neuroblastoma, and sarcoma. As an immune suppressor it is used in nephrotic syndrome and following organ transplant. It is taken by mouth or injection into a vein. The main effect of cyclophosphamide is due to its metabolite phosphoramide mustard. This metabolite is only formed in cells that have low levels of ALDH. Phosphoramide mustard forms DNA crosslinks both between and within DNA strands at guanine N-7 positions (known as interstrand and intrastrand crosslinkages, respectively). This is irreversible and leads to cell apoptosis.

Chemical Structure

Cyclophosphamide monohydrate
CAS# 6055-19-2 (hydrate)

Theoretical Analysis

MedKoo Cat#: 100190
Name: Cyclophosphamide monohydrate
CAS#: 6055-19-2 (hydrate)
Chemical Formula: C7H15Cl2N2O2P
Exact Mass:
Molecular Weight: 279.10124
Elemental Analysis: C, 32.20; H, 5.79; Cl, 27.16; N, 10.73; O, 12.26; P, 11.86

Price and Availability

Size Price Availability Quantity
2.0g USD 150.0 Same day
5.0g USD 250.0 Same day
10.0g USD 450.0 Same day
25.0g USD 650.0 2 weeks
50.0g USD 950.0 2 weeks
100.0g USD 1750.0 2 weeks
Bulk inquiry

Related CAS #: 6055-19-2 (hydrate)   50-18-0 (anhydrous)    

Synonym: Ciclofosfamida; Ciclofosfamide; Cyclophosphamide monohydrate.

IUPAC/Chemical Name: 2-[bis(2-chloroethyl)amino]tetrahydro-2H-1,3,2-oxazaphosphorine 2-oxide monohydrate


InChi Code: InChI=1S/C7H15Cl2N2O2P.H2O/c8-2-5-11(6-3-9)14(12)10-4-1-7-13-14;/h1-7H2,(H,10,12);1H2


Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, not in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Certificate of Analysis:

Safety Data Sheet (SDS):

Preparing Stock Solutions

The following data is based on the product molecular weight 279.10124 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

1: Rodríguez PC, Rodríguez G, González G, Lage A. Clinical development and perspectives of CIMAvax EGF, Cuban vaccine for non-small-cell lung cancer therapy. MEDICC Rev. 2010 Winter;12(1):17-23. Review. PubMed PMID: 20387330.

2: Teicher BA. In vivo/ex vivo and in situ assays used in cancer research: a brief review. Toxicol Pathol. 2009;37(1):114-22. Epub 2008 Dec 19. Review. PubMed PMID: 19098118.

3: Ballatori E, Roila F, Ruggeri B, De Angelis V, Porzio G, Marchetti P; Italian Group for Antiemetic Research. Inappropriate doses of chemotherapy in Italian breast cancer patients enrolled in clinical trials. Tumori. 2007 Nov-Dec;93(6):540-3. PubMed PMID: 18338486.

4: Chen CS, Jounaidi Y, Su T, Waxman DJ. Enhancement of intratumoral cyclophosphamide pharmacokinetics and antitumor activity in a P450 2B11-based cancer gene therapy model. Cancer Gene Ther. 2007 Dec;14(12):935-44. Epub 2007 Sep 14. PubMed PMID: 17853921; PubMed Central PMCID: PMC2613860.

5: Liang J, Huang M, Duan W, Yu XQ, Zhou S. Design of new oxazaphosphorine anticancer drugs. Curr Pharm Des. 2007;13(9):963-78. Review. PubMed PMID: 17430192.

6: Hou DY, Muller AJ, Sharma MD, DuHadaway J, Banerjee T, Johnson M, Mellor AL, Prendergast GC, Munn DH. Inhibition of indoleamine 2,3-dioxygenase in dendritic cells by stereoisomers of 1-methyl-tryptophan correlates with antitumor responses. Cancer Res. 2007 Jan 15;67(2):792-801. PubMed PMID: 17234791.

7: Miki T, Mizutani Y, Akaza H, Ozono S, Tsukamoto T, Terachi T, Naito K, Nonomura N, Hara I, Yoshida O; Japan Blood Cell Transplantation Study Group for Testicular Germ Cell Tumor. Long-term results of first-line sequential high-dose carboplatin, etoposide and ifosfamide chemotherapy with peripheral blood stem cell support for patients with advanced testicular germ cell tumor. Int J Urol. 2007 Jan;14(1):54-9. PubMed PMID: 17199861.

8: Stapleton S, Blaney S. New agents for intrathecal administration. Cancer Invest. 2006 Aug-Sep;24(5):528-34. Review. PubMed PMID: 16939963.

9: Motl S, Zhuang Y, Waters CM, Stewart CF. Pharmacokinetic considerations in the treatment of CNS tumours. Clin Pharmacokinet. 2006;45(9):871-903. Review. PubMed PMID: 16928151.

10: Zhang J, Tian Q, Zhu YZ, Xu AL, Zhou SF. Reversal of resistance to oxazaphosphorines. Curr Cancer Drug Targets. 2006 Aug;6(5):385-407. Review. PubMed PMID: 16918308.

Additional Information

Related CAS#
6055-19-2 (Cyclophosphamide hydrate);
50-18-0 (Cyclophosphamide anhydrous)