WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 406579

CAS#: 1418033-25-6

Description: LMK-235 is a selective histone deacetylase (HDAC) 4 and HDAC5 inhibitor. LMK-235 demonstrates activity against chemoresistant cancer cell lines in an MTT assay for cytotoxicity using human ovarian cancer cell lines A2780 and cisplatin resistant A2780CisR (IC50 = 0.49 and 0.32 μ M respectively).

Chemical Structure

CAS# 1418033-25-6

Theoretical Analysis

MedKoo Cat#: 406579
Name: LMK-235
CAS#: 1418033-25-6
Chemical Formula: C15H22N2O4
Exact Mass: 294.16
Molecular Weight: 294.350
Elemental Analysis: C, 61.21; H, 7.53; N, 9.52; O, 21.74

Price and Availability

Size Price Availability Quantity
10mg USD 95 Ready to ship
25mg USD 200 Ready to ship
50mg USD 350 Ready to ship
100mg USD 550 Ready to ship
200mg USD 950 Ready to ship
500mg USD 2050 Ready to ship
1g USD 3250 Ready to ship
2g USD 5850 Ready to ship
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Synonym: LMK235; LMK 235; LMK-235.

IUPAC/Chemical Name: N-((6-(hydroxyamino)-6-oxohexyl)oxy)-3,5-dimethylbenzamide


InChi Code: InChI=1S/C15H22N2O4/c1-11-8-12(2)10-13(9-11)15(19)17-21-7-5-3-4-6-14(18)16-20/h8-10,20H,3-7H2,1-2H3,(H,16,18)(H,17,19)


Appearance: White to off-white solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, not in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Biological target: LMK-235 is a potent and selective HDAC4/5 inhibitor, inhibits HDAC5, HDAC4, HDAC6, HDAC1, HDAC2, HDAC11 and HDAC8, with IC50s of 4.22 nM, 11.9 nM, 55.7 nM, 320 nM, 881 nM, 852 nM and 1278 nM, respectively.
In vitro activity: It was found that LMK-235, a selective inhibitor of class IIA HDAC4/5, induced apoptosis of MM cells by downregulating HO-1 that is closely related to HDAC4. LMK-235 increased phosphorylation of JNK and c -Jun in MM cells. Downregulation of HO-1 expression in combination with LMK-235 expression further activated phosphorylation of JNK and c-Jun and induced apoptosis in MM cells. When the JNK inhibitor SP600125 was used in combination, the apoptosis phenomenon was reversed. However, when HO-1 was upregulated, LMK-235-mediated phosphorylation of JNK and c-Jun was inhibited, and apoptosis of MM cells began to decrease. These data suggest that LMK-235 has potent anti-myeloma activity through regulation of HO-1-induced apoptosis via the JNK/AP-1 pathway. This provides a new concept for the treatment of multiple myeloma. Reference: Life Sci. 2019 Apr 15;223:146-157. https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(19)30169-9
In vivo activity: The effects of 2 doses (5 and 20 mg/kg) of LMK235 on H3 acetylation were examined. It was found that an 8-day treatment with the lowest dose (5 mg/kg) was sufficient to fully restore H3 acetylation in the hippocampus of Cdkl5 -/Y mice (Fig. 7B). A moderate increase in H3 acetylation was also found in control mice (data not shown). Based on these results, Cdkl5 -/Y and control Cdkl5 +/Y male mice were treated daily with the lowest dose of LMK235 or vehicle. Some mice were sacrificed after 8 days of treatment. Other mice were treated for 8 days plus additional 8 days during which they were behaviorally tested. These mice were sacrificed immediately after behavioral testing (i.e. 16 days after initiation of treatment; Fig. 7A). The effects of treatment on the neuroanatomy of the hippocampal region were examined in mice treated for 8 and 16 days. It was found that both 8 and 16 days of treatment had no adverse effect on body weight in both genotypes indicating that LMK235 does not impair animals' well-being (Supplementary Material, Table 1). Reference: Hum Mol Genet. 2016 Sep 15;25(18):3887-3907. https://academic.oup.com/hmg/article-lookup/doi/10.1093/hmg/ddw231

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 29.4 100.00
Ethanol 29.4 100.00

Preparing Stock Solutions

The following data is based on the product molecular weight 294.350000000000000000000000000000 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol: 1. Li X, Guo Y, Kuang X, Zhao L, Li H, Cheng B, Wang W, Zhang Z, Liu P, Wang J. Histone deacetylase inhibitor LMK-235-mediated HO-1 expression induces apoptosis in multiple myeloma cells via the JNK/AP-1 signaling pathway. Life Sci. 2019 Apr 15;223:146-157. doi: 10.1016/j.lfs.2019.03.011. Epub 2019 Mar 12. PMID: 30876940. 2. Li A, Liu Z, Li M, Zhou S, Xu Y, Xiao Y, Yang W. HDAC5, a potential therapeutic target and prognostic biomarker, promotes proliferation, invasion and migration in human breast cancer. Oncotarget. 2016 Jun 21;7(25):37966-37978. doi: 10.18632/oncotarget.9274. Erratum in: Oncotarget. 2017 May 2;8(18):30619-30620. PMID: 27177225; PMCID: PMC5122364.
In vivo protocol: 1. Trazzi S, Fuchs C, Viggiano R, De Franceschi M, Valli E, Jedynak P, Hansen FK, Perini G, Rimondini R, Kurz T, Bartesaghi R, Ciani E. HDAC4: a key factor underlying brain developmental alterations in CDKL5 disorder. Hum Mol Genet. 2016 Sep 15;25(18):3887-3907. doi: 10.1093/hmg/ddw231. Epub 2016 Jul 27. PMID: 27466189.

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1: Hansen FK, Sumanadasa SD, Stenzel K, Duffy S, Meister S, Marek L, Schmetter R, Kuna K, Hamacher A, Mordmüller B, Kassack MU, Winzeler EA, Avery VM, Andrews KT, Kurz T. Discovery of HDAC inhibitors with potent activity against multiple malaria parasite life cycle stages. Eur J Med Chem. 2014 Jul 23;82:204-13. doi: 10.1016/j.ejmech.2014.05.050. Epub 2014 May 22. PubMed PMID: 24904967. 2: Marek L, Hamacher A, Hansen FK, Kuna K, Gohlke H, Kassack MU, Kurz T. Histone deacetylase (HDAC) inhibitors with a novel connecting unit linker region reveal a selectivity profile for HDAC4 and HDAC5 with improved activity against chemoresistant cancer cells. J Med Chem. 2013 Jan 24;56(2):427-36. doi: 10.1021/jm301254q. Epub 2013 Jan 8. PubMed PMID: 23252603.