L319
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MedKoo CAT#: 465715

CAS#: 1351586-50-9

Description: L319, also known as O-8670, Lipid 319; RV-92, is an ionizable lipid. It is useful for developing lipid nanoparticles (LNP) for siRNA, mRNA, and vaccine delivery. Lipid 319 nanoparticles have better delivery efficacy and biodegradability than DLin-MC3-DMA in the delivery of mRNA in vivo. MC3 is the key delivery nanoparticle component of the first siRNA drug Onpattro.


Chemical Structure

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L319
CAS# 1351586-50-9

Theoretical Analysis

MedKoo Cat#: 465715
Name: L319
CAS#: 1351586-50-9
Chemical Formula: C41H75NO6
Exact Mass: 677.56
Molecular Weight: 678.050
Elemental Analysis: C, 72.63; H, 11.15; N, 2.07; O, 14.16

Price and Availability

Size Price Availability Quantity
5mg USD 150 Ready to Ship
10mg USD 250 Ready to Ship
25mg USD 550 Ready to ship
50mg USD 900 Ready to ship
100mg USD 1650 Ready to Ship
500mg USD 2450 Ready to Ship
1g USD 3850 Ready to Ship
2g USD 5850 Ready to Ship
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Synonym: L319; L 319; L-319; O-8670; O8670; O 8670; RV-92; RV 92; RV92; Lipid 319;

IUPAC/Chemical Name: Di((Z)-Non-2-en-1-yl) 9-((4-(dimethylamino)butanoyl)oxy)heptadecanedioate

InChi Key: DGNMJYUPWDTKJB-ZDSKVHJSSA-N

InChi Code: InChI=1S/C41H75NO6/c1-5-7-9-11-13-21-27-36-46-39(43)32-25-19-15-17-23-30-38(48-41(45)34-29-35-42(3)4)31-24-18-16-20-26-33-40(44)47-37-28-22-14-12-10-8-6-2/h21-22,27-28,38H,5-20,23-26,29-37H2,1-4H3/b27-21-,28-22-

SMILES Code: O=C(OC/C=C\CCCCCC)CCCCCCCC(OC(CCCN(C)C)=O)CCCCCCCC(OC/C=C\CCCCCC)=O

Appearance: Liquid

Purity: >95% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target: L319 is an ionizable cationic lipidoid and can be used for synthetic liposomes.
In vitro activity: Combining F-L319 and L319 as hybrid ionizable lipids in LNPs greatly enhanced mRNA delivery efficiency both in vitro and in vivo. Mechanistic studies indicate that the enhanced mRNA delivery of the hybrid-LNP is attributed to both improved mRNA encapsulation and cellular uptake. Reference: J Mater Chem B. 2023 May 17;11(19):4171-4180. https://pubmed.ncbi.nlm.nih.gov/37129135/
In vivo activity: Uridine replacement with N1-methylpseudouridine in an mRNA vaccine encoding influenza hemagglutinin had a significant impact on the induction of innate chemokines/cytokines and a positive impact on the induction of functional antibody titers in mice and macaques when MC3 or KC2 LNPs were used as the delivery system. However, it only minimally impacted the titers obtained with L319 LNPs. The impact of nucleoside modification on mRNA vaccine efficacy varies with LNP composition. Reference: Mol Ther Nucleic Acids. 2023 May 8;32:794-806. https://pubmed.ncbi.nlm.nih.gov/37346973/

Preparing Stock Solutions

The following data is based on the product molecular weight 678.05 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Huo H, Cheng X, Xu J, Lin J, Chen N, Lu X. A fluorinated ionizable lipid improves the mRNA delivery efficiency of lipid nanoparticles. J Mater Chem B. 2023 May 17;11(19):4171-4180. doi: 10.1039/d3tb00516j. PMID: 37129135. 2. Bernard MC, Bazin E, Petiot N, Lemdani K, Commandeur S, Verdelet C, Margot S, Perkov V, Ripoll M, Garinot M, Ruiz S, Boudet F, Rokbi B, Haensler J. The impact of nucleoside base modification in mRNA vaccine is influenced by the chemistry of its lipid nanoparticle delivery system. Mol Ther Nucleic Acids. 2023 May 8;32:794-806. doi: 10.1016/j.omtn.2023.05.004. PMID: 37346973; PMCID: PMC10280092.
In vitro protocol: 1. Huo H, Cheng X, Xu J, Lin J, Chen N, Lu X. A fluorinated ionizable lipid improves the mRNA delivery efficiency of lipid nanoparticles. J Mater Chem B. 2023 May 17;11(19):4171-4180. doi: 10.1039/d3tb00516j. PMID: 37129135.
In vivo protocol: 1. Bernard MC, Bazin E, Petiot N, Lemdani K, Commandeur S, Verdelet C, Margot S, Perkov V, Ripoll M, Garinot M, Ruiz S, Boudet F, Rokbi B, Haensler J. The impact of nucleoside base modification in mRNA vaccine is influenced by the chemistry of its lipid nanoparticle delivery system. Mol Ther Nucleic Acids. 2023 May 8;32:794-806. doi: 10.1016/j.omtn.2023.05.004. PMID: 37346973; PMCID: PMC10280092.

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1. Huo H, Cheng X, Xu J, Lin J, Chen N, Lu X. A fluorinated ionizable lipid improves the mRNA delivery efficiency of lipid nanoparticles. J Mater Chem B. 2023 May 17;11(19):4171-4180. doi: 10.1039/d3tb00516j. PMID: 37129135.

2. Bernard MC, Bazin E, Petiot N, Lemdani K, Commandeur S, Verdelet C, Margot S, Perkov V, Ripoll M, Garinot M, Ruiz S, Boudet F, Rokbi B, Haensler J. The impact of nucleoside base modification in mRNA vaccine is influenced by the chemistry of its lipid nanoparticle delivery system. Mol Ther Nucleic Acids. 2023 May 8;32:794-806. doi: 10.1016/j.omtn.2023.05.004. PMID: 37346973; PMCID: PMC10280092.