WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 510295

CAS#: 12648-68-7

Description: U-73122 is a potent phospholipase C inhibitor or PI-PLC inhibitor. U-73122 is widely used as a tool to investigate the involvement of the phosphoinositide-specific phospholipase C (PLC) in signal transduction, particularly in studies attempting to characterize pathways leading to intracellular Ca2+ mobilization upon agonist challenge. U-73122 directly blocks PLC isoenzymes in vivo and in vitro.

Price and Availability


USD 1650
USD 4750

USD 2150
USD 5950

USD 2950
Ask price

U73122, purity > 98%, is in stock. Current shipping out time is about 2 weeks after order is received. CoA, QC data and MSDS documents are available in one week after order is received. Delivery time: overnight (USA/Canada); 3-5 days (worldwide).

Chemical Structure


Theoretical Analysis

MedKoo Cat#: 510295
Name: U-73122
CAS#: 12648-68-7
Chemical Formula: C29H40N2O3
Exact Mass: 464.30389
Molecular Weight: 464.64
Elemental Analysis: C, 74.96; H, 8.68; N, 6.03; O, 10.33

Synonym: U73122, U 73122, U73122.

IUPAC/Chemical Name: 1-(6-(((8R,9S,13S,14S,17S)-3-methoxy-13-methyl-7,8,9,11,12,13,14,15,16,17-decahydro-6H-cyclopenta[a]phenanthren-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione


InChi Code: InChI=1S/C29H40N2O3/c1-29-16-15-23-22-10-8-21(34-2)19-20(22)7-9-24(23)25(29)11-12-26(29)30-17-5-3-4-6-18-31-27(32)13-14-28(31)33/h8,10,13-14,19,23-26,30H,3-7,9,11-12,15-18H2,1-2H3/t23-,24-,25+,26+,29+/m1/s1

SMILES Code: O=C(C=C1)N(CCCCCCN[C@H]2CC[C@@]3([H])[C@]4([H])CCC5=CC(OC)=CC=C5[C@@]4([H])CC[C@]23C)C1=O

Technical Data

Solid powder

>98% (or refer to the Certificate of Analysis)

Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition:
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Soluble in DMSO, not in water

Shelf Life:
>2 years if stored properly

Drug Formulation:
This drug may be formulated in DMSO

Stock Solution Storage:
0 - 4 C for short term (days to weeks), or -20 C for long term (months).

Harmonized System Code:


1: Lo Vasco VR, Leopizzi M, Di Maio V, Della Rocca C. U-73122 reduces the cell growth in cultured MG-63 ostesarcoma cell line involving Phosphoinositide-specific Phospholipases C. Springerplus. 2016 Feb 24;5:156. doi: 10.1186/s40064-016-1768-6. eCollection 2016. PubMed PMID: 27026853; PubMed Central PMCID: PMC4766154.

2: Hughes SA, Gibson WJ, Young JM. The interaction of U-73122 with the histamine H1 receptor: implications for the use of U-73122 in defining H1 receptor-coupled signalling pathways. Naunyn Schmiedebergs Arch Pharmacol. 2000 Dec;362(6):555-8. PubMed PMID: 11138848.

3: Hollywood MA, Sergeant GP, Thornbury KD, McHale NG. The PI-PLC inhibitor U-73122 is a potent inhibitor of the SERCA pump in smooth muscle. Br J Pharmacol. 2010 Jul;160(6):1293-4. doi: 10.1111/j.1476-5381.2010.00795.x. PubMed PMID: 20590620; PubMed Central PMCID: PMC2938801.

4: Chen YJ, Sheng WY, Huang PR, Wang TC. Potent inhibition of human telomerase by U-73122. J Biomed Sci. 2006 Sep;13(5):667-74. Epub 2006 Jul 19. PubMed PMID: 16850179.

5: Chen TH, Hsu WH. U-73122 inhibits carbachol-induced increases in [Ca2+]i, IP3, and insulin release in beta-TC3 cells. Life Sci. 1995;56(5):PL103-8. PubMed PMID: 7837924.

6: Wiciński M, Grześk G, Malinowski B, Grześk E, Sinjab AT, Krzyzanowski M, Michalska A, Bylina D, Dziecioł J, Szadujkis-Szadurska K, Czeczuk A. Imidazole-induced contractility of vascular smooth muscle cells in the presence of U-73122, ODQ, indomethacin and 7-nitroindazole. Pol J Vet Sci. 2013;16(2):293-7. PubMed PMID: 23971197.

7: Feisst C, Albert D, Steinhilber D, Werz O. The aminosteroid phospholipase C antagonist U-73122 (1-[6-[[17-beta-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5- dione) potently inhibits human 5-lipoxygenase in vivo and in vitro. Mol Pharmacol. 2005 May;67(5):1751-7. Epub 2005 Jan 31. PubMed PMID: 15684042.

8: Smith RJ, Justen JM, McNab AR, Rosenbloom CL, Steele AN, Detmers PA, Anderson DC, Manning AM. U-73122: a potent inhibitor of human polymorphonuclear neutrophil adhesion on biological surfaces and adhesion-related effector functions. J Pharmacol Exp Ther. 1996 Jul;278(1):320-9. PubMed PMID: 8764366.

9: Thompson AK, Mostafapour SP, Denlinger LC, Bleasdale JE, Fisher SK. The aminosteroid U-73122 inhibits muscarinic receptor sequestration and phosphoinositide hydrolysis in SK-N-SH neuroblastoma cells. A role for Gp in receptor compartmentation. J Biol Chem. 1991 Dec 15;266(35):23856-62. PubMed PMID: 1660886.

10: Alter CA, Amagasu M, Shah K, Jolly YC, Major C, Wolf BA. U-73122 does not specifically inhibit phospholipase C in rat pancreatic islets and insulin-secreting beta-cell lines. Life Sci. 1994;54(8):PL107-12. PubMed PMID: 8107526.

11: Grierson JP, Meldolesi J. Calcium homeostasis in mouse fibroblast cells: affected by U-73122, a putative phospholipase C beta blocker, via multiple mechanisms. Br J Pharmacol. 1995 May;115(1):11-4. PubMed PMID: 7647965; PubMed Central PMCID: PMC1908738.

12: Lo Vasco VR, Fabrizi C, Panetta B, Fumagalli L, Cocco L. Expression pattern and sub-cellular distribution of phosphoinositide specific phospholipase C enzymes after treatment with U-73122 in rat astrocytoma cells. J Cell Biochem. 2010 Jul 1;110(4):1005-12. doi: 10.1002/jcb.22614. PubMed PMID: 20564200.

13: Li SB, Chen TH, Zhu-Ge RH, Hsu WH. Effect of U-73122 on calcium channel activity in porcine myometrial cells and pancreatic beta-cell line RINm5F. Zhongguo Yao Li Xue Bao. 1997 Sep;18(5):385-90. PubMed PMID: 10322922.

14: Macmillan D, McCarron JG. The phospholipase C inhibitor U-73122 inhibits Ca(2+) release from the intracellular sarcoplasmic reticulum Ca(2+) store by inhibiting Ca(2+) pumps in smooth muscle. Br J Pharmacol. 2010 Jul;160(6):1295-301. doi: 10.1111/j.1476-5381.2010.00771.x. PubMed PMID: 20590621; PubMed Central PMCID: PMC2938802.

15: Wang JP. U-73122, an aminosteroid phospholipase C inhibitor, may also block Ca2+ influx through phospholipase C-independent mechanism in neutrophil activation. Naunyn Schmiedebergs Arch Pharmacol. 1996 May;353(6):599-605. PubMed PMID: 8738291.

16: Wang XD, Kiang JG, Smallridge RC. A phospholipase C inhibitor, U-73122, blocks TSH-induced inositol trisphosphate production, Ca2+ increase and arachidonic acid release in FRTL-5 thyroid cells. Biochim Biophys Acta. 1994 Aug 11;1223(1):101-6. PubMed PMID: 8061042.

17: Smallridge RC, Kiang JG, Gist ID, Fein HG, Galloway RJ. U-73122, an aminosteroid phospholipase C antagonist, noncompetitively inhibits thyrotropin-releasing hormone effects in GH3 rat pituitary cells. Endocrinology. 1992 Oct;131(4):1883-8. PubMed PMID: 1396332.

18: Badawi JK, Seja T, Bross S. In vitro effects of the cyclooxygenase inhibitor indomethacin and of the phospholipase-C inhibitor U-73122 on carbachol-induced contractions of porcine detrusor muscle. Fundam Clin Pharmacol. 2008 Dec;22(6):667-72. doi: 10.1111/j.1472-8206.2008.00625.x. PubMed PMID: 19049671.

19: Nagy K, Contzen K. Inhibition of phospholipase C by U-73122 blocks one component of the receptor current in Limulus photoreceptor. Vis Neurosci. 1997 Sep-Oct;14(5):995-8. PubMed PMID: 9364735.

20: Salari H, Bramley A, Langlands J, Howard S, Chan-Yeung M, Chan H, Schellenberg R. Effect of phospholipase C inhibitor U-73122 on antigen-induced airway smooth muscle contraction in guinea pigs. Am J Respir Cell Mol Biol. 1993 Oct;9(4):405-10. PubMed PMID: 8398179.