Oxythiamine Free Base

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 414701

CAS#: 136-16-3 (free base)

Description: Oxythiamine Free Base is a thiamine antagonist, antimetabolite.

Chemical Structure

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Oxythiamine Free Base
CAS# 136-16-3 (free base)
Instruction

Theoretical Analysis

MedKoo Cat#: 414701
Name: Oxythiamine Free Base
CAS#: 136-16-3 (free base)
Chemical Formula: C12H16N3O2S+
Exact Mass: 266.10
Molecular Weight: 266.340
Elemental Analysis: C, 54.12; H, 6.06; N, 15.78; O, 12.01; S, 12.04

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

Related CAS #: 136-16-3 (free base); 614-05-1 (chloride HCl)  

Synonym: Oxythiamine Free Base; BRN4153910; BRN-4153910; BRN 4153910

IUPAC/Chemical Name: Thiazolium, 3-((1,4-dihydro-2-methyl-4-oxo-5-pyrimidinyl)methyl)-5-(2-hydroxyethyl)-4-methyl-

InChi Key: SRDGSXVLAVRBLU-UHFFFAOYSA-O

InChi Code: InChI=1S/C12H15N3O2S/c1-8-11(3-4-16)18-7-15(8)6-10-5-13-9(2)14-12(10)17/h5,7,16H,3-4,6H2,1-2H3/p+1

SMILES Code: CC1=C(CCO)SC=[N+]1CC2=CNC(C)=NC2=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 266.34 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Golan O, Dyer R, Sinclair G, Blydt-Hansen T. Investigating oxythiamine levels in children undergoing kidney transplantation and the risk of immediate post- operative metabolic and hemodynamic decompensation. Pediatr Nephrol. 2021 Apr;36(4):987-993. doi: 10.1007/s00467-020-04797-9. Epub 2020 Oct 16. PMID: 33067673.

2: Thiamine and oxythiamine metabolism. Nutr Rev. 1969 Mar;27(3):88-90. doi: 10.1111/j.1753-4887.1969.tb04972.x. PMID: 4891096.

3: Siemieniuk M, Sosnowska K, Czerniecki J, Czyzewska U, Winnicka K, Tylicki A. Oxythiamine improves antifungal activity of ketoconazole evaluated in canine Malassezia pachydermatis strains. Vet Dermatol. 2018 Dec;29(6):476-e160. doi: 10.1111/vde.12688. Epub 2018 Sep 24. PMID: 30251451.

4: Zhang F, Masania J, Anwar A, Xue M, Zehnder D, Kanji H, Rabbani N, Thornalley PJ. The uremic toxin oxythiamine causes functional thiamine deficiency in end- stage renal disease by inhibiting transketolase activity. Kidney Int. 2016 Aug;90(2):396-403. doi: 10.1016/j.kint.2016.03.010. Epub 2016 May 16. PMID: 27198804.

5: Vinogradov VV, Vodoevich VP, Rozhko AV. Iavliaetsia li oksitiamin antivitaminom? [Is oxythiamine an antivitamin?]. Biokhimiia. 1995 Oct;60(10):1564-74. Russian. PMID: 8555356.

6: Tylicki A, Czerniecki J, Dobrzyn P, Matanowska A, Olechno A, Strumilo S. Modification of thiamine pyrophosphate dependent enzyme activity by oxythiamine in Saccharomyces cerevisiae cells. Can J Microbiol. 2005 Oct;51(10):833-9. doi: 10.1139/w05-072. PMID: 16333342.

7: Szoka L, Karna E, Palka J. The mechanism of oxythiamine-induced collagen biosynthesis in cultured fibroblasts. Mol Cell Biochem. 2015 May;403(1-2):51-60. doi: 10.1007/s11010-015-2336-z. Epub 2015 Jan 28. PMID: 25626895; PMCID: PMC4383821.

8: Grabowska E, Czerniecka M, Czyżewska U, Zambrzycka A, Łotowski Z, Tylicki A. Differences in the efficiency of 3-deazathiamine and oxythiamine pyrophosphates as inhibitors of pyruvate dehydrogenase complex and growth of HeLa cells in vitro. J Enzyme Inhib Med Chem. 2021 Dec;36(1):122-129. doi: 10.1080/14756366.2020.1844681. PMID: 33187452; PMCID: PMC7671598.

9: Tseng CW, Kuo WH, Chan SH, Chan HL, Chang KJ, Wang LH. Transketolase Regulates the Metabolic Switch to Control Breast Cancer Cell Metastasis via the α-Ketoglutarate Signaling Pathway. Cancer Res. 2018 Jun 1;78(11):2799-2812. doi: 10.1158/0008-5472.CAN-17-2906. Epub 2018 Mar 29. PMID: 29599405.

10: Yang CM, Liu YZ, Liao JW, Hu ML. The in vitro and in vivo anti-metastatic efficacy of oxythiamine and the possible mechanisms of action. Clin Exp Metastasis. 2010 May;27(5):341-9. doi: 10.1007/s10585-010-9331-2. Epub 2010 May 7. PMID: 20449639.