RB394 | CAS# 1830320-32-5 | Dual sEH/PPARγ modulator

RB394
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 464126

CAS#: 1830320-32-5

Description: RB394 is a dual sEH/PPARγ modulator.

Chemical Structure

RB394

CAS# 1830320-32-5

Product data Instruction

Theoretical Analysis

MedKoo Cat#: 464126
Name: RB394
CAS#: 1830320-32-5
Chemical Formula: C21H22F3NO4
Exact Mass: 409.15
Molecular Weight: 409.410
Elemental Analysis: C, 61.61; H, 5.42; F, 13.92; N, 3.42; O, 15.63

Price and Availability

Size	Price	Availability
50mg	USD -2	2 Weeks
100mg	USD -2	2 Weeks
200mg	USD -2	2 Weeks
500mg	USD -2	2 Weeks
2g	USD -2	2 Weeks
1mg	USD 210	2 Weeks
5mg	USD 400	2 Weeks
10mg	USD 650	2 Weeks
25mg	USD 1090	2 Weeks
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: RB394; RB 394; RB-394;

IUPAC/Chemical Name: 2-(4-((4-methoxy-2-(trifluoromethyl)benzyl)carbamoyl)benzyl)butanoic acid

InChi Key: JUYLDNKPEWYVEB-UHFFFAOYSA-N

InChi Code: InChI=1S/C21H22F3NO4/c1-3-14(20(27)28)10-13-4-6-15(7-5-13)19(26)25-12-16-8-9-17(29-2)11-18(16)21(22,23)24/h4-9,11,14H,3,10,12H2,1-2H3,(H,25,26)(H,27,28)

SMILES Code: COC1=CC(C(F)(F)F)=C(CNC(C2=CC=C(CC(C(O)=O)CC)C=C2)=O)C=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, DMF, and ethanol

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO, DMF, and ethanol

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:

Product Data

Instruction:

View handling instruction

Biological target:	RB394 is an orally bioavailable, dual modulator of soluble epoxide hydrolase (sEH) and PPARγ that inhibits sEH with an IC50 of 0.33 µM and activates PPARγ with an EC50 of 0.3 µM. It is inactive at PPARδ and shows 29% activation of PPARα, relative to control, at 10 µM. RB394 is water soluble, orally bioavailable, and can be delivered through drinking water.
In vitro activity:	To be determined
In vivo activity:	RB394 can attenuate renal fibrosis in a mouse unilateral ureteral obstruction model by reducing renal inflammation, oxidative stress, tubular injury, and vascular injury. RB394 demonstrates exciting potential as a therapeutic for renal fibrosis and chronic kidney disease. Reference: Prostaglandins Other Lipid Mediat. 2020 Oct;150:106472. https://pubmed.ncbi.nlm.nih.gov/32569747/

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	33.0	80.60
	DMF	25.0	61.06
	Ethanol	5.0	12.21

Preparing Stock Solutions

The following data is based on the product molecular weight 409.41 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:	1. Hartmann M, Bibli SI, Tews D, Ni X, Kircher T, Kramer JS, Kilu W, Heering J, Hernandez-Olmos V, Weizel L, Scriba GKE, Krait S, Knapp S, Chaikuad A, Merk D, Fleming I, Fischer-Posovszky P, Proschak E. Combined Cardioprotective and Adipocyte Browning Effects Promoted by the Eutomer of Dual sEH/PPARγ Modulator. J Med Chem. 2021 Mar 11;64(5):2815-2828. doi: 10.1021/acs.jmedchem.0c02063. Epub 2021 Feb 23. PMID: 33620196. 2. Stavniichuk A, Hye Khan MA, Yeboah MM, Chesnik MA, Jankiewicz WK, Hartmann M, Blöcher R, Kircher T, Savchuk O, Proschak E, Imig JD. Dual soluble epoxide hydrolase inhibitor/PPAR-γ agonist attenuates renal fibrosis. Prostaglandins Other Lipid Mediat. 2020 Oct;150:106472. doi: 10.1016/j.prostaglandins.2020.106472. Epub 2020 Jun 20. PMID: 32569747. 3. Hye Khan MA, Kolb L, Skibba M, Hartmann M, Blöcher R, Proschak E, Imig JD. A novel dual PPAR-γ agonist/sEH inhibitor treats diabetic complications in a rat model of type 2 diabetes. Diabetologia. 2018 Oct;61(10):2235-2246. doi: 10.1007/s00125-018-4685-0. Epub 2018 Jul 21. PMID: 30032428; PMCID: PMC6563928.
In vitro protocol:	To be determined
In vivo protocol:	1. Hartmann M, Bibli SI, Tews D, Ni X, Kircher T, Kramer JS, Kilu W, Heering J, Hernandez-Olmos V, Weizel L, Scriba GKE, Krait S, Knapp S, Chaikuad A, Merk D, Fleming I, Fischer-Posovszky P, Proschak E. Combined Cardioprotective and Adipocyte Browning Effects Promoted by the Eutomer of Dual sEH/PPARγ Modulator. J Med Chem. 2021 Mar 11;64(5):2815-2828. doi: 10.1021/acs.jmedchem.0c02063. Epub 2021 Feb 23. PMID: 33620196. 2. Stavniichuk A, Hye Khan MA, Yeboah MM, Chesnik MA, Jankiewicz WK, Hartmann M, Blöcher R, Kircher T, Savchuk O, Proschak E, Imig JD. Dual soluble epoxide hydrolase inhibitor/PPAR-γ agonist attenuates renal fibrosis. Prostaglandins Other Lipid Mediat. 2020 Oct;150:106472. doi: 10.1016/j.prostaglandins.2020.106472. Epub 2020 Jun 20. PMID: 32569747. 3. Hye Khan MA, Kolb L, Skibba M, Hartmann M, Blöcher R, Proschak E, Imig JD. A novel dual PPAR-γ agonist/sEH inhibitor treats diabetic complications in a rat model of type 2 diabetes. Diabetologia. 2018 Oct;61(10):2235-2246. doi: 10.1007/s00125-018-4685-0. Epub 2018 Jul 21. PMID: 30032428; PMCID: PMC6563928.

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1: Hartmann M, Bibli SI, Tews D, Ni X, Kircher T, Kramer JS, Kilu W, Heering J, Hernandez-Olmos V, Weizel L, Scriba GKE, Krait S, Knapp S, Chaikuad A, Merk D, Fleming I, Fischer-Posovszky P, Proschak E. Combined Cardioprotective and Adipocyte Browning Effects Promoted by the Eutomer of Dual sEH/PPARγ Modulator. J Med Chem. 2021 Feb 23. doi: 10.1021/acs.jmedchem.0c02063. Epub ahead of print. PMID: 33620196.

2: Stavniichuk A, Hye Khan MA, Yeboah MM, Chesnik MA, Jankiewicz WK, Hartmann M, Blöcher R, Kircher T, Savchuk O, Proschak E, Imig JD. Dual soluble epoxide hydrolase inhibitor/PPAR-γ agonist attenuates renal fibrosis. Prostaglandins Other Lipid Mediat. 2020 Oct;150:106472. doi: 10.1016/j.prostaglandins.2020.106472. Epub 2020 Jun 20. PMID: 32569747.

3: Hye Khan MA, Kolb L, Skibba M, Hartmann M, Blöcher R, Proschak E, Imig JD. A novel dual PPAR-γ agonist/sEH inhibitor treats diabetic complications in a rat model of type 2 diabetes. Diabetologia. 2018 Oct;61(10):2235-2246. doi: 10.1007/s00125-018-4685-0. Epub 2018 Jul 21. PMID: 30032428; PMCID: PMC6563928.

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