VU0285655-1
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MedKoo CAT#: 412719

CAS#: 1158347-73-9

Description: VU0285655-1 or APV (1158347-73-9) is a phospholipase D2 (PLD2) inhibitor. It induces autophagy in colorectal cancer cells.


Chemical Structure

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VU0285655-1
CAS# 1158347-73-9

Theoretical Analysis

MedKoo Cat#: 412719
Name: VU0285655-1
CAS#: 1158347-73-9
Chemical Formula: C25H27N5O2
Exact Mass: 429.2165
Molecular Weight: 429.52
Elemental Analysis: C, 69.91; H, 6.34; N, 16.31; O, 7.45

Price and Availability

Size Price Availability Quantity
1.0mg USD 328.0 2 Weeks
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Synonym: VU0285655-1; N-{2-[4-oxo-1-phenyl-1,3,8-triazaspiro(4.5)decan-8-yl]ethyl}quinoline-3-carboxamide

IUPAC/Chemical Name: N-(2-(4-oxo-1-phenyl-1,3,8-triazaspiro[4.5]decan-8-yl)ethyl)quinoline-3-carboxamide

InChi Key: WJOCDBUFEUKYNI-UHFFFAOYSA-N

InChi Code: InChI=1S/C25H27N5O2/c31-23(20-16-19-6-4-5-9-22(19)27-17-20)26-12-15-29-13-10-25(11-14-29)24(32)28-18-30(25)21-7-2-1-3-8-21/h1-9,16-17H,10-15,18H2,(H,26,31)(H,28,32)

SMILES Code: O=C(C1=CN=C(C=CC=C2)C2=C1)NCCN(CC3)CCC43N(C5=CC=CC=C5)CNC4=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 429.52 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1: Burkhardt U, Beyer S, Klein J. Role of phospholipases D1 and 2 in astroglial proliferation: effects of specific inhibitors and genetic deletion. Eur J Pharmacol. 2015 Aug 15;761:398-404. doi: 10.1016/j.ejphar.2015.05.004. Epub 2015 May 9. PMID: 25967349.

2: Burkhardt U, Wojcik B, Zimmermann M, Klein J. Phospholipase D is a target for inhibition of astroglial proliferation by ethanol. Neuropharmacology. 2014 Apr;79:1-9. doi: 10.1016/j.neuropharm.2013.11.002. Epub 2013 Nov 19. PMID: 24262632.

3: Bermúdez V, Tenconi PE, Giusto NM, Mateos MV. Lipopolysaccharide-Induced Autophagy Mediates Retinal Pigment Epithelium Cells Survival. Modulation by the Phospholipase D Pathway. Front Cell Neurosci. 2019 Apr 24;13:154. doi: 10.3389/fncel.2019.00154. PMID: 31327962; PMCID: PMC6497095.

4: Tenconi PE, Bermúdez V, Oresti GM, Giusto NM, Salvador GA, Mateos MV. High glucose-induced phospholipase D activity in retinal pigment epithelium cells: New insights into the molecular mechanisms of diabetic retinopathy. Exp Eye Res. 2019 Jul;184:243-257. doi: 10.1016/j.exer.2019.04.028. Epub 2019 May 3. PMID: 31059692.

5: Tsai YR, Huang LJ, Lin HY, Hung YJ, Lee MR, Kuo SC, Hsu MF, Wang JP. Inhibition of formyl peptide-stimulated phospholipase D activation by Fal-002-2 via blockade of the Arf6, RhoA and protein kinase C signaling pathways in rat neutrophils. Naunyn Schmiedebergs Arch Pharmacol. 2013 Jun;386(6):507-19. doi: 10.1007/s00210-013-0851-6. Epub 2013 Mar 23. PMID: 23525454.

6: Hwang WC, Kim MK, Song JH, Choi KY, Min do S. Inhibition of phospholipase D2 induces autophagy in colorectal cancer cells. Exp Mol Med. 2014 Dec 5;46(12):e124. doi: 10.1038/emm.2014.74. PMID: 25475140; PMCID: PMC4274395.



Additional Information

Hygroscopic