para-NitroBlebbistatin
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MedKoo CAT#: 463499

CAS#: 1621326-32-6

Description: para-NitroBlebbistatin is a selective cell-permeable inhibitor of non-muscle myosin II ATPases, being more stable and less phototoxic than Blebbistatin.

Chemical Structure

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para-NitroBlebbistatin
CAS# 1621326-32-6
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 463499
Name: para-NitroBlebbistatin
CAS#: 1621326-32-6
Chemical Formula: C18H15N3O4
Exact Mass: 337.11
Molecular Weight: 337.335
Elemental Analysis: C, 64.09; H, 4.48; N, 12.46; O, 18.97

Price and Availability

Size Price Availability Quantity
1mg USD 280 2 Weeks
5mg USD 670 2 Weeks
10mg USD 1160 2 Weeks
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Synonym: para-NitroBlebbistatin; para NitroBlebbistatin; para-Nitro Blebbistatin; para-Nitro-Blebbistatin; (–)-4'-amino-Blebbistatin; p-amino-Blebbistatin; (S)-4'-amino-Blebbistatin;

IUPAC/Chemical Name: (S)-3a-hydroxy-6-methyl-1-(4-nitrophenyl)-1,2,3,3a-tetrahydro-4H-pyrrolo[2,3-b]quinolin-4-one

InChi Key: KAUXNLHXGQGFOS-GOSISDBHSA-N

InChi Code: InChI=1S/C18H15N3O4/c1-11-2-7-15-14(10-11)16(22)18(23)8-9-20(17(18)19-15)12-3-5-13(6-4-12)21(24)25/h2-7,10,23H,8-9H2,1H3/t18-/m1/s1

SMILES Code: O=C1[C@@]2(O)C(N(C3=CC=C([N+]([O-])=O)C=C3)CC2)=NC4=C1C=C(C)C=C4

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Instruction:
View handling instruction
Biological target: para-Nitroblebbistatin is a non-cytotoxic, photostable, fluorescent and specific Myosin II inhibitor.
In vitro activity: TBD
In vivo activity: TBD

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 20.0 59.29
DMF:PBS (pH 7.2) (1:1) 0.5 1.48
DMSO 17.3 51.14

Preparing Stock Solutions

The following data is based on the product molecular weight 337.34 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: TBD
In vitro protocol: TBD
In vivo protocol: TBD

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1: Tang W, Blair CA, Walton SD, Málnási-Csizmadia A, Campbell KS, Yengo CM. Modulating Beta-Cardiac Myosin Function at the Molecular and Tissue Levels. Front Physiol. 2017 Jan 9;7:659. doi: 10.3389/fphys.2016.00659. PMID: 28119616; PMCID: PMC5220080.

2: Várkuti BH, Képiró M, Horváth IÁ, Végner L, Ráti S, Zsigmond Á, Hegyi G, Lenkei Z, Varga M, Málnási-Csizmadia A. A highly soluble, non-phototoxic, non- fluorescent blebbistatin derivative. Sci Rep. 2016 May 31;6:26141. doi: 10.1038/srep26141. PMID: 27241904; PMCID: PMC4886532.

3: Képiró M, Várkuti BH, Végner L, Vörös G, Hegyi G, Varga M, Málnási-Csizmadia A. para-Nitroblebbistatin, the non-cytotoxic and photostable myosin II inhibitor. Angew Chem Int Ed Engl. 2014 Jul 28;53(31):8211-5. doi: 10.1002/anie.201403540. Epub 2014 Jun 20. PMID: 24954740.