FAPGG

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 463311

CAS#: 64967-39-1

Description: FAPGG is a substrate for angiotensin converting enzyme (ACE).


Chemical Structure

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FAPGG
CAS# 64967-39-1

Theoretical Analysis

MedKoo Cat#: 463311
Name: FAPGG
CAS#: 64967-39-1
Chemical Formula: C20H21N3O6
Exact Mass: 399.14
Molecular Weight: 399.403
Elemental Analysis: C, 60.14; H, 5.30; N, 10.52; O, 24.03

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

Request quote for custom synthesis

Synonym: FAPGG;

IUPAC/Chemical Name: (E)-(E)-(3-(furan-2-yl)acryloyl)-L-phenylalanylglycylglycine

InChi Key: ZDLZKMDMBBMJLI-FDMDGMSGSA-N

InChi Code: InChI=1S/C20H21N3O6/c24-17(9-8-15-7-4-10-29-15)23-16(11-14-5-2-1-3-6-14)20(28)22-12-18(25)21-13-19(26)27/h1-10,16H,11-13H2,(H,21,25)(H,22,28)(H,23,24)(H,26,27)/b9-8+/t16-/m0/s1

SMILES Code: O=C(O)CNC(CNC([C@@H](NC(/C=C/C1=CC=CO1)=O)CC2=CC=CC=C2)=O)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 399.40 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Baudin B, Zendjabil M, Allouche S, Soudan B, Boutarfa N, Bénéteau-Burnat B, Potestat K, Caussé E, Chaabouni T, Ibrahim F, Taibi L. Measurement of angiotensin-I-converting enzyme in the blood: help for method validation. Ann Biol Clin (Paris). 2020 Aug 1;78(4):454-460. English. doi: 10.1684/abc.2020.1568. PMID: 32616472.

2: Arise RO, Acho MA, Yekeen AA, Omokanye IA, Sunday-Nwaso EO, Akiode OS, Malomo SO. Kinetics of angiotensin -1 converting enzyme inhibition and antioxidative properties of Azadirachta indica seed protein hydrolysates. Heliyon. 2019 May 27;5(5):e01747. doi: 10.1016/j.heliyon.2019.e01747. PMID: 31193663; PMCID: PMC6538965.

3: Giromini C, Fekete ÁA, Givens DI, Baldi A, Lovegrove JA. Short-Communication: A Comparison of the In Vitro Angiotensin-1-Converting Enzyme Inhibitory Capacity of Dairy and Plant Protein Supplements. Nutrients. 2017 Dec 13;9(12):1352. doi: 10.3390/nu9121352. PMID: 29236035; PMCID: PMC5748802.

4: Tanzadehpanah H, Asoodeh A, Saidijam M, Chamani J, Mahaki H. Improving efficiency of an angiotensin converting enzyme inhibitory peptide as multifunctional peptides. J Biomol Struct Dyn. 2018 Nov;36(14):3803-3818. doi: 10.1080/07391102.2017.1401001. Epub 2017 Dec 7. PMID: 29173094.

5: Asoodeh A, Homayouni-Tabrizi M, Shabestarian H, Emtenani S, Emtenani S. Biochemical characterization of a novel antioxidant and angiotensin I-converting enzyme inhibitory peptide from Struthio camelus egg white protein hydrolysis. J Food Drug Anal. 2016 Apr;24(2):332-342. doi: 10.1016/j.jfda.2015.11.010. Epub 2016 Feb 23. PMID: 28911587.

6: Culjak M, Nikolac N, Simundic AM. Short-term storage stability of serum angiotensin-converting enzyme (ACE) activity. Clin Chim Acta. 2015 Jun 15;446:261-2. doi: 10.1016/j.cca.2015.05.001. Epub 2015 May 7. PMID: 25957986.

7: Fagyas M, Úri K, Siket IM, Daragó A, Boczán J, Bányai E, Édes I, Papp Z, Tóth A. New perspectives in the renin-angiotensin-aldosterone system (RAAS) I: endogenous angiotensin converting enzyme (ACE) inhibition. PLoS One. 2014 Apr 1;9(4):e87843. doi: 10.1371/journal.pone.0087843. PMID: 24691160; PMCID: PMC3972180.

8: Ben Henda Y, Labidi A, Arnaudin I, Bridiau N, Delatouche R, Maugard T, Piot JM, Sannier F, Thiéry V, Bordenave-Juchereau S. Measuring angiotensin-I converting enzyme inhibitory activity by micro plate assays: comparison using marine cryptides and tentative threshold determinations with captopril and losartan. J Agric Food Chem. 2013 Nov 13;61(45):10685-90. doi: 10.1021/jf403004e. Epub 2013 Oct 31. PMID: 24131339.

9: Beyazit Y, Purnak T, Suvak B, Kurt M, Sayilir A, Turhan T, Tas A, Torun S, Celik T, Ibis M, Haznedaroglu IC. Increased ACE in extrahepatic cholangiocarcinoma as a clue for activated RAS in biliary neoplasms. Clin Res Hepatol Gastroenterol. 2011 Oct;35(10):644-9. doi: 10.1016/j.clinre.2011.06.008. Epub 2011 Jul 29. PMID: 21802387.

10: Costa MF, Carmona AK, Alves MF, Ryan TM, Davies HM, Anderson GA, Slocombe RF. Determination of angiotensin I-converting enzyme activity in equine blood: lack of agreement between methods of analysis. J Vet Sci. 2011 Mar;12(1):21-5. doi: 10.4142/jvs.2011.12.1.21. PMID: 21368559; PMCID: PMC3053463.

11: Chen HL, Lünsdorf H, Hecht HJ, Tsai H. Porcine pulmonary angiotensin I-converting enzyme--biochemical characterization and spatial arrangement of the N- and C-domains by three-dimensional electron microscopic reconstruction. Micron. 2010 Aug;41(6):674-85. doi: 10.1016/j.micron.2010.01.005. Epub 2010 Mar 17. PMID: 20427191.

12: Quassinti L, Maccari E, Murri O, Bramucci M. Comparison of ACE activity in amphibian tissues: Rana esculenta and Xenopus laevis. Comp Biochem Physiol A Mol Integr Physiol. 2007 Jan;146(1):119-23. doi: 10.1016/j.cbpa.2006.09.016. Epub 2006 Oct 3. PMID: 17081790.

13: Shalaby SM, Zakora M, Otte J. Performance of two commonly used angiotensin- converting enzyme inhibition assays using FA-PGG and HHL as substrates. J Dairy Res. 2006 May;73(2):178-86. doi: 10.1017/S0022029905001639. Epub 2006 Feb 14. PMID: 16476177.

14: Vercruysse L, Smagghe G, Herregods G, Van Camp J. ACE inhibitory activity in enzymatic hydrolysates of insect protein. J Agric Food Chem. 2005 Jun 29;53(13):5207-11. doi: 10.1021/jf050337q. PMID: 15969498.

15: Miano A, Quassinti L, Maccari E, Murri O, Amici D, Bramucci M. Purified angiotensin converting enzyme from Rana esculenta ovary influences ovarian steroidogenesis in vitro. J Physiol Biochem. 2003 Dec;59(4):269-76. doi: 10.1007/BF03179884. PMID: 15164946.

16: Murray BA, Walsh DJ, FitzGerald RJ. Modification of the furanacryloyl-L- phenylalanylglycylglycine assay for determination of angiotensin-I-converting enzyme inhibitory activity. J Biochem Biophys Methods. 2004 May 31;59(2):127-37. doi: 10.1016/j.jbbm.2003.12.009. PMID: 15163524.

17: Zhang XH, Ma XJ, Luo J, Shao JH, Geng QX, Zhu XL, Tang YS, Xu FY. [Effect of different angiotensin converting enzyme inhibitors on coronary collateral circulation]. Zhonghua Yi Xue Za Zhi. 2004 Apr 2;84(7):592-5. Chinese. PMID: 15144597.

18: Actis-Goretta L, Ottaviani JI, Keen CL, Fraga CG. Inhibition of angiotensin converting enzyme (ACE) activity by flavan-3-ols and procyanidins. FEBS Lett. 2003 Dec 18;555(3):597-600. doi: 10.1016/s0014-5793(03)01355-3. PMID: 14675780.

19: Binevski PV, Sizova EA, Pozdnev VF, Kost OA. Evidence for the negative cooperativity of the two active sites within bovine somatic angiotensin- converting enzyme. FEBS Lett. 2003 Aug 28;550(1-3):84-8. doi: 10.1016/s0014-5793(03)00825-1. PMID: 12935891.

20: Hou WC, Chen HJ, Lin YH. Antioxidant peptides with Angiotensin converting enzyme inhibitory activities and applications for Angiotensin converting enzyme purification. J Agric Food Chem. 2003 Mar 12;51(6):1706-9. doi: 10.1021/jf0260242. PMID: 12617609.