Calpain inhibitor III

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 412245

CAS#: 88191-84-8

Description: Calpain inhibitor III is a potential anticataract drug.

Chemical Structure

Calpain inhibitor III
CAS# 88191-84-8

Theoretical Analysis

MedKoo Cat#: 412245
Name: Calpain inhibitor III
CAS#: 88191-84-8
Chemical Formula: C21H24N2O4
Exact Mass: 368.1736
Molecular Weight: 368.43
Elemental Analysis: C, 68.46; H, 6.57; N, 7.60; O, 17.37

Price and Availability

Size Price Availability Quantity
25.0mg USD 205.0 2 Weeks
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Synonym: Calpain inhibitor III; MDL28170; MDL 28170 MDL-28170

IUPAC/Chemical Name: Carbamic acid, ((1S)-1-((((1S)-1-formyl-2-phenylethyl)amino)carbonyl)-2-methylpropyl)-, phenyl ester


InChi Code: InChI=1S/C21H24N2O4/c1-15(2)19(23-21(26)27-18-11-7-4-8-12-18)20(25)22-17(14-24)13-16-9-5-3-6-10-16/h3-12,14-15,17,19H,13H2,1-2H3,(H,22,25)(H,23,26)/t17-,19-/m0/s1


Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 368.43 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1: Liang L, Li H, Cao T, Qu L, Zhang L, Fan GC, Greer PA, Li J, Jones DL, Peng T. Calpain activation mediates microgravity-induced myocardial abnormalities in mice via p38 and ERK1/2 MAPK pathways. J Biol Chem. 2020 Sep 28:jbc.RA119.011890. doi: 10.1074/jbc.RA119.011890. Epub ahead of print. PMID: 32989050.

2: Sun X, Sun Y, Jiang P, Qi G, Chen X. Crosstalk between endothelial cell- specific calpain inhibition and the endothelial-mesenchymal transition via the HSP90/Akt signaling pathway. Biomed Pharmacother. 2020 Apr;124:109822. doi: 10.1016/j.biopha.2020.109822. Epub 2020 Jan 17. PMID: 31958767.

3: Chen L, Wang L, Li X, Wang C, Hong M, Li Y, Cao J, Fu L. The role of desmin alterations in mechanical electrical feedback in heart failure. Life Sci. 2020 Jan 15;241:117119. doi: 10.1016/j.lfs.2019.117119. Epub 2019 Nov 30. PMID: 31794771.

4: Liu W, Zhou Y, Liang R, Zhang Y. Inhibition of cyclin-dependent kinase 5 activity alleviates diabetes-related cognitive deficits. FASEB J. 2019 Dec;33(12):14506-14515. doi: 10.1096/fj.201901292R. Epub 2019 Nov 5. PMID: 31689375.

5: Ennes-Vidal V, Vitório BDS, Menna-Barreto RFS, Pitaluga AN, Gonçalves-da- Silva SA, Branquinha MH, Santos ALS, d'Avila-Levy CM. Calpains of Leishmania braziliensis: genome analysis, differential expression, and functional analysis. Mem Inst Oswaldo Cruz. 2019 Sep 23;114:e190147. doi: 10.1590/0074-02760190147. PMID: 31553371; PMCID: PMC6759280.

6: Chen Q, Thompson J, Hu Y, Dean J, Lesnefsky EJ. Inhibition of the ubiquitous calpains protects complex I activity and enables improved mitophagy in the heart following ischemia-reperfusion. Am J Physiol Cell Physiol. 2019 Nov 1;317(5):C910-C921. doi: 10.1152/ajpcell.00190.2019. Epub 2019 Aug 14. PMID: 31411917; PMCID: PMC6879877.

7: Nie Q, Zhu L, Zhang L, Leng B, Wang H. Astragaloside IV protects against hyperglycemia-induced vascular endothelial dysfunction by inhibiting oxidative stress and Calpain-1 activation. Life Sci. 2019 Sep 1;232:116662. doi: 10.1016/j.lfs.2019.116662. Epub 2019 Jul 16. PMID: 31323271.

8: He J, Deng F, Pan D, Zeng X. Investigation of the relationships between different enzymes and postmortem duck muscle tenderization. Poult Sci. 2019 Nov 1;98(11):6125-6130. doi: 10.3382/ps/pez301. PMID: 31198938.

9: González-Hernández M, Müller A, Hoenen T, Hoffmann M, Pöhlmann S. Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L. Virology. 2019 Jun;532:22-29. doi: 10.1016/j.virol.2019.03.020. Epub 2019 Apr 3. PMID: 30999160; PMCID: PMC7112014.

10: Hu J, Chen L, Huang X, Wu K, Ding S, Wang W, Wang B, Smith C, Ren C, Ni H, ZhuGe Q, Yang J. Calpain inhibitor MDL28170 improves the transplantation- mediated therapeutic effect of bone marrow-derived mesenchymal stem cells following traumatic brain injury. Stem Cell Res Ther. 2019 Mar 15;10(1):96. doi: 10.1186/s13287-019-1210-4. PMID: 30876457; PMCID: PMC6420775.

11: Shi D, He T, Tang W, Li H, Wang C, Zheng M, Hu J, Song X, Ding Y, Chen YY, Shen Y, Jin H, Wang LL. Local application of MDL28170-loaded PCL film improves functional recovery by preserving survival of motor neurons after traumatic spinal cord injury. Neurosci Lett. 2019 Feb 16;694:161-167. doi: 10.1016/j.neulet.2018.12.006. Epub 2018 Dec 5. PMID: 30528875.

12: Pierre JS, Giblot-Ducray D, McKay AC, Hartley DM, Perroux JM, Rae AL. DNA based diagnostic for the quantification of sugarcane root DNA in the field. Sci Rep. 2018 Nov 13;8(1):16720. doi: 10.1038/s41598-018-34844-3. PMID: 30425256; PMCID: PMC6233164.

13: Giakoumettis D, Pourzitaki C, Vavilis T, Tsingotjidou A, Kyriakoudi A, Tsimidou M, Boziki M, Sioga A, Foroglou N, Kritis A. Crocus sativus L. Causes a Non Apoptotic Calpain Dependent Death in C6 Rat Glioma Cells, Exhibiting a Synergistic Effect with Temozolomide. Nutr Cancer. 2019;71(3):491-507. doi: 10.1080/01635581.2018.1506493. Epub 2018 Oct 1. PMID: 30273051.

14: Yang M, Chen W, Zhang Y, Yang R, Wang Y, Yuan H. EphrinB/EphB signaling contributes to spinal nociceptive processing via calpain‑1 and caspase‑3. Mol Med Rep. 2018 Jul;18(1):268-278. doi: 10.3892/mmr.2018.8996. Epub 2018 May 9. PMID: 29749521; PMCID: PMC6059679.

15: de Sousa Araújo PS, de Oliveira SSC, d'Avila-Levy CM, Dos Santos ALS, Branquinha MH. Susceptibility of promastigotes and intracellular amastigotes from distinct Leishmania species to the calpain inhibitor MDL28170. Parasitol Res. 2018 Jul;117(7):2085-2094. doi: 10.1007/s00436-018-5894-7. Epub 2018 May 4. PMID: 29728827.

16: Li H, Chen LP, Wang T, Wang SG, Liu JH. Calpain inhibition improves erectile function in diabetic mice via upregulating endothelial nitric oxide synthase expression and reducing apoptosis. Asian J Androl. 2018 Jul-Aug;20(4):342-348. doi: 10.4103/aja.aja_63_17. PMID: 29319007; PMCID: PMC6038160.

17: Dao CV, Shiraishi M, Miyamoto A. The MARCKS protein amount is differently regulated by calpain during toxic effects of methylmercury between SH-SY5Y and EA.hy926 cells. J Vet Med Sci. 2017 Dec 6;79(12):1931-1938. doi: 10.1292/jvms.17-0473. Epub 2017 Oct 18. PMID: 29046508; PMCID: PMC5745167.

18: Oliveira SSC, Gonçalves IC, Ennes-Vidal V, Lopes AHCS, Menna-Barreto RFS, D'Ávila-Levy CM, Santos ALS, Branquinha MH. In vitro selection of Phytomonas serpens cells resistant to the calpain inhibitor MDL28170: alterations in fitness and expression of the major peptidases and efflux pumps. Parasitology. 2018 Mar;145(3):355-370. doi: 10.1017/S0031182017001561. Epub 2017 Oct 17. PMID: 29039273.

19: Yuan XC, Wu CH, Gao F, Li HP, Xiang HC, Zhu H, Pan XL, Lin LX, Liu YS, Yu W, Tian B, Meng XF, Li M. Activation and expression of μ-calpain in dorsal root contributes to RTX-induced mechanical allodynia. Mol Pain. 2017 Jan- Dec;13:1744806917719169. doi: 10.1177/1744806917719169. PMID: 28714350; PMCID: PMC5548329.

20: Marinho FA, Sangenito LS, Oliveira SSC, De Arruda LB, D'Ávila-Levy CM, Santos ALS, Branquinha MH. The potent cell permeable calpain inhibitor MDL28170 affects the interaction of Leishmania amazonensis with macrophages and shows anti-amastigote activity. Parasitol Int. 2017 Oct;66(5):579-583. doi: 10.1016/j.parint.2017.06.010. Epub 2017 Jun 27. PMID: 28663009.