ID-8 inhibitor
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 555860

CAS#: 147591-46-6

Description: ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture.


Chemical Structure

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ID-8 inhibitor
CAS# 147591-46-6

Theoretical Analysis

MedKoo Cat#: 555860
Name: ID-8 inhibitor
CAS#: 147591-46-6
Chemical Formula: C16H14N2O4
Exact Mass: 298.10
Molecular Weight: 298.298
Elemental Analysis: C, 64.42; H, 4.73; N, 9.39; O, 21.45

Price and Availability

Size Price Availability Quantity
50mg USD 450 2 Weeks
100mg USD 750 2 Weeks
200mg USD 1250 2 Weeks
500mg USD 2650 2 Weeks
1g USD 3650 2 Weeks
2g USD 6450 2 Weeks
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Synonym: ID-8 inhibitor; ID 8 inhibitor; ID8 inhibitor;

IUPAC/Chemical Name: 1-(4-methoxyphenyl)-2-methyl-3-nitro-1H-indol-6-ol

InChi Key: VVZNWYXIOADGSW-UHFFFAOYSA-N

InChi Code: InChI=1S/C16H14N2O4/c1-10-16(18(20)21)14-8-5-12(19)9-15(14)17(10)11-3-6-13(22-2)7-4-11/h3-9,19H,1-2H3

SMILES Code: OC1=CC2=C(C=C1)C([N+]([O-])=O)=C(C)N2C3=CC=C(OC)C=C3

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Biological target: ID-8 is an inhibitor of dual-specificity tyrosine phosphorylation-regulated kinase (DYRK). ID-8 sustains embryonic stem cell (ESC) self-renewal and pluripotency. ID-8 enhances Wnt-mediated hESC survival and proliferation via inhibition of DYRKs.
In vitro activity: As expected, levels of the CBP/β-catenin complex were significantly enhanced in ID-8-treated cells compared with control and in particular to ICG-001-treated cells. A similar result was obtained in DYRK-knockdown cells (Fig. 2I, 2J). This study concludes that ID-8, through inhibition of DYRK family members, supports maintenance of the undifferentiated state in the presence of Wnt by modulating Wnt/β-catenin signaling and enhancing the CBP/β-catenin association in hESCs, similar to the mechanism observed in mESCs treated with IQ-1 and Wnt. Reference: Stem Cells Transl Med. 2012 Jan;1(1):18-28. https://pubmed.ncbi.nlm.nih.gov/23197636/
In vivo activity: TBD

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 33.3 111.63
DMF:PBS (pH 7.2) (1:2) 0.3 1.11
DMSO 44.0 147.50
Ethanol 2.0 6.70

Preparing Stock Solutions

The following data is based on the product molecular weight 298.30 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Monteiro MB, Ramm S, Chandrasekaran V, Boswell SA, Weber EJ, Lidberg KA, Kelly EJ, Vaidya VS. A High-Throughput Screen Identifies DYRK1A Inhibitor ID-8 that Stimulates Human Kidney Tubular Epithelial Cell Proliferation. J Am Soc Nephrol. 2018 Dec;29(12):2820-2833. doi: 10.1681/ASN.2018040392. Epub 2018 Oct 25. PMID: 30361326; PMCID: PMC6287872. 2. Hasegawa K, Yasuda SY, Teo JL, Nguyen C, McMillan M, Hsieh CL, Suemori H, Nakatsuji N, Yamamoto M, Miyabayashi T, Lutzko C, Pera MF, Kahn M. Wnt signaling orchestration with a small molecule DYRK inhibitor provides long-term xeno-free human pluripotent cell expansion. Stem Cells Transl Med. 2012 Jan;1(1):18-28. doi: 10.5966/sctm.2011-0033. Epub 2011 Dec 7. PMID: 23197636; PMCID: PMC3727690.
In vitro protocol: 1. Monteiro MB, Ramm S, Chandrasekaran V, Boswell SA, Weber EJ, Lidberg KA, Kelly EJ, Vaidya VS. A High-Throughput Screen Identifies DYRK1A Inhibitor ID-8 that Stimulates Human Kidney Tubular Epithelial Cell Proliferation. J Am Soc Nephrol. 2018 Dec;29(12):2820-2833. doi: 10.1681/ASN.2018040392. Epub 2018 Oct 25. PMID: 30361326; PMCID: PMC6287872. 2. Hasegawa K, Yasuda SY, Teo JL, Nguyen C, McMillan M, Hsieh CL, Suemori H, Nakatsuji N, Yamamoto M, Miyabayashi T, Lutzko C, Pera MF, Kahn M. Wnt signaling orchestration with a small molecule DYRK inhibitor provides long-term xeno-free human pluripotent cell expansion. Stem Cells Transl Med. 2012 Jan;1(1):18-28. doi: 10.5966/sctm.2011-0033. Epub 2011 Dec 7. PMID: 23197636; PMCID: PMC3727690.
In vivo protocol: TBD

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1: Monteiro MB, Ramm S, Chandrasekaran V, Boswell SA, Weber EJ, Lidberg KA, Kelly EJ, Vaidya VS. A High-Throughput Screen Identifies DYRK1A Inhibitor ID-8 that Stimulates Human Kidney Tubular Epithelial Cell Proliferation. J Am Soc Nephrol. 2018 Dec;29(12):2820-2833. doi: 10.1681/ASN.2018040392. Epub 2018 Oct 25. PMID: 30361326; PMCID: PMC6287872.


2: Sylvain V, Lafarge S, Bignon YJ. Molecular pathways involved in response to ionizing radiation of ID-8 mouse ovarian cancer cells expressing exogenous full- length Brca1 or truncated Brca1 mutant. Int J Oncol. 2001 Sep;19(3):599-607. doi: 10.3892/ijo.19.3.599. PMID: 11494042.