LIN28 inhibitor LI71
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MedKoo CAT#: 555829

CAS#: 1357248-83-9

Description: LIN28 inhibitor LI71 is a potent, cell-permeable inhibitor of LIN28 that abolishes LIN28-mediated oligouridylation. LIN28 inhibitor LI71 binds the cold shock domain to suppress LIN28's activity against let-7 in leukemia cells and embryonic stem cells.

Chemical Structure

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LIN28 inhibitor LI71
CAS# 1357248-83-9
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 555829
Name: LIN28 inhibitor LI71
CAS#: 1357248-83-9
Chemical Formula: C21H21NO3
Exact Mass: 335.15
Molecular Weight: 335.403
Elemental Analysis: C, 75.20; H, 6.31; N, 4.18; O, 14.31

Price and Availability

Size Price Availability Quantity
100mg USD 2950 2 Weeks
200mg USD 3950 2 Weeks
500mg USD 5950 2 Weeks
1g USD 8950 2 Weeks
2g USD 16950 2 Weeks
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Synonym: LIN28 inhibitor LI71; L-I71; L I71;

IUPAC/Chemical Name: 4-((3aS,4R,9bR)-6-ethoxy-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinolin-4-yl)benzoic acid

InChi Key: QWJMABCFVYELBB-GJYPPUQNSA-N

InChi Code: InChI=1S/C21H21NO3/c1-2-25-18-8-4-7-17-15-5-3-6-16(15)19(22-20(17)18)13-9-11-14(12-10-13)21(23)24/h3-5,7-12,15-16,19,22H,2,6H2,1H3,(H,23,24)/t15-,16+,19+/m1/s1

SMILES Code: O=C(O)C1=CC=C([C@@H]2NC3=C(C=CC=C3OCC)[C@@]4([H])[C@]2([H])CC=C4)C=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Instruction:
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Biological target: LIN28 inhibitor LI71 is a potent and cell-permeable LIN28 inhibitor, which abolishes LIN28-mediated oligouridylation with an IC50 of 7 uM.
In vitro activity: LI71 could significantly reduce the relative Renilla luciferase activity in cells expressing LIN28A and LIN28B at 50–100 μM (Figures 6B and 6C). Together, these results demonstrate that, consistent with in vitro data, LI71 inhibits LIN28’s activity on let-7 micro-RNAs in LIN28-dependent cancer and embryonic stem cells. Reference: Cell Rep. 2018 Jun 5;23(10):3091-3101. https://pubmed.ncbi.nlm.nih.gov/29874593/
In vivo activity: TBD

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 100.0 298.15

Preparing Stock Solutions

The following data is based on the product molecular weight 335.40 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Wang L, Rowe RG, Jaimes A, Yu C, Nam Y, Pearson DS, Zhang J, Xie X, Marion W, Heffron GJ, Daley GQ, Sliz P. Small-Molecule Inhibitors Disrupt let-7 Oligouridylation and Release the Selective Blockade of let-7 Processing by LIN28. Cell Rep. 2018 Jun 5;23(10):3091-3101. doi: 10.1016/j.celrep.2018.04.116. PMID: 29874593; PMCID: PMC6511231.
In vitro protocol: 1. Wang L, Rowe RG, Jaimes A, Yu C, Nam Y, Pearson DS, Zhang J, Xie X, Marion W, Heffron GJ, Daley GQ, Sliz P. Small-Molecule Inhibitors Disrupt let-7 Oligouridylation and Release the Selective Blockade of let-7 Processing by LIN28. Cell Rep. 2018 Jun 5;23(10):3091-3101. doi: 10.1016/j.celrep.2018.04.116. PMID: 29874593; PMCID: PMC6511231.
In vivo protocol: TBD

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