WARNING: This product is for research use only, not for human or veterinary use.
MedKoo CAT#: 574310
CAS#: 1416561-90-4
Description: O-Propargyl-puromycin is a clickable form of the protein synthesis inhibitor puromycin. OP-puro is incorporated into nascent polypeptide chains where it forms covalent adducts and terminates chain elongation on the ribosome. It has been used in combination with fluorescent azides to image nascent proteins in various cells.
MedKoo Cat#: 574310
Name: O-Propargyl-puromycin
CAS#: 1416561-90-4
Chemical Formula: C24H29N7O5
Exact Mass: 495.223
Molecular Weight: 495.54
Elemental Analysis: C, 58.17; H, 5.90; N, 19.79; O, 16.14
Synonym: O-Propargyl-puromycin, OPP, OP-puro
IUPAC/Chemical Name: 3'-[[(2S)-2-amino-1-oxo-3-[4-(2-propyn-1-yloxy)phenyl]propyl]amino]-3'-deoxy-N,N-dimethyl-adenosine
InChi Key: JXBIGWQNNSJLQK-IYRMOJGWSA-N
InChi Code: InChI=1S/C24H29N7O5/c1-4-9-35-15-7-5-14(6-8-15)10-16(25)23(34)29-18-17(11-32)36-24(20(18)33)31-13-28-19-21(30(2)3)26-12-27-22(19)31/h1,5-8,12-13,16-18,20,24,32-33H,9-11,25H2,2-3H3,(H,29,34)/t16-,17+,18+,20+,24+/m0/s1
SMILES Code: O[C@H]1[C@H](N2C=NC3=C2N=CN=C3N(C)C)O[C@H](CO)[C@H]1NC([C@@H](N)CC4=CC=C(OCC#C)C=C4)=O
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >3 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
The following data is based on the product molecular weight 495.54 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
1. Liu, J., Xu, Y., Stoleru, D., et al. Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. Proc. Natl. Acad. Sci. USA 109(2), 413-418 (2012).
2. Forester, C.M., Zhao, Q., Phillips, N.J., et al. Revealing nascent proteomics in signaling pathways and cell differentiation. Proc. Natl. Acad. Sci. USA 115(10), 2353-2358 (2018).
