O-Propargyl-puromycin
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 574310

CAS#: 1416561-90-4

Description: O-Propargyl-puromycin is a clickable form of the protein synthesis inhibitor puromycin. OP-puro is incorporated into nascent polypeptide chains where it forms covalent adducts and terminates chain elongation on the ribosome. It has been used in combination with fluorescent azides to image nascent proteins in various cells.

Chemical Structure

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O-Propargyl-puromycin
CAS# 1416561-90-4
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 574310
Name: O-Propargyl-puromycin
CAS#: 1416561-90-4
Chemical Formula: C24H29N7O5
Exact Mass: 495.22
Molecular Weight: 495.540
Elemental Analysis: C, 58.17; H, 5.90; N, 19.79; O, 16.14

Price and Availability

Size Price Availability Quantity
1mg USD 460 2 Weeks
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Synonym: O-Propargyl-puromycin, OPP, OP-puro

IUPAC/Chemical Name: 3'-[[(2S)-2-amino-1-oxo-3-[4-(2-propyn-1-yloxy)phenyl]propyl]amino]-3'-deoxy-N,N-dimethyl-adenosine

InChi Key: JXBIGWQNNSJLQK-IYRMOJGWSA-N

InChi Code: InChI=1S/C24H29N7O5/c1-4-9-35-15-7-5-14(6-8-15)10-16(25)23(34)29-18-17(11-32)36-24(20(18)33)31-13-28-19-21(30(2)3)26-12-27-22(19)31/h1,5-8,12-13,16-18,20,24,32-33H,9-11,25H2,2-3H3,(H,29,34)/t16-,17+,18+,20+,24+/m0/s1

SMILES Code: O[C@H]1[C@H](N2C=NC3=C2N=CN=C3N(C)C)O[C@H](CO)[C@H]1NC([C@@H](N)CC4=CC=C(OCC#C)C=C4)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Instruction:
View handling instruction
Biological target: O-Propargyl-Puromycin, an alkyne analog of puromycin, is a potent protein synthesis inhibitor.
In vitro activity: TBD
In vivo activity: TBD

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 40.3 81.27

Preparing Stock Solutions

The following data is based on the product molecular weight 495.54 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: TBD
In vitro protocol: TBD
In vivo protocol: TBD

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1. Liu, J., Xu, Y., Stoleru, D., et al. Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. Proc. Natl. Acad. Sci. USA 109(2), 413-418 (2012).

2. Forester, C.M., Zhao, Q., Phillips, N.J., et al. Revealing nascent proteomics in signaling pathways and cell differentiation. Proc. Natl. Acad. Sci. USA 115(10), 2353-2358 (2018).