LysoFP-NH2
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MedKoo CAT#: 574185

CAS#: 69408-85-1

Description: LysoFP-NH2 is the fluorescent form of the lysosomal turn-on fluorescent probe for carbon monoxide (CO) lysoFP-NO2. LysoFP-NH2 is formed when lysoFP-NO2 reacts with CO. It displays excitation/emission maxima of 440/528 nm, respectively.

Chemical Structure

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LysoFP-NH2
CAS# 69408-85-1
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 574185
Name: LysoFP-NH2
CAS#: 69408-85-1
Chemical Formula: C18H19N3O3
Exact Mass: 325.14
Molecular Weight: 325.368
Elemental Analysis: C, 66.45; H, 5.89; N, 12.91; O, 14.75

Price and Availability

Size Price Availability Quantity
5mg USD 350 2 weeks
10mg USD 650 2 weeks
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Synonym: LysoFP-NH2

IUPAC/Chemical Name: 5-amino-2-[2-(4-morpholinyl)ethyl]-1H-benz[de]isoquinoline-1,3(2H)-dione

InChi Key: JYKPDVFVKOXSSF-UHFFFAOYSA-N

InChi Code: InChI=1S/C18H19N3O3/c19-13-10-12-2-1-3-14-16(12)15(11-13)18(23)21(17(14)22)5-4-20-6-8-24-9-7-20/h1-3,10-11H,4-9,19H2

SMILES Code: NC1=CC(C2=O)=C3C(C(N2CCN4CCOCC4)=O)=CC=CC3=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info: Emission: 528 nm Excitation: 440 nm

Product Data:
Product Data
Instruction:
View handling instruction
Biological target: LysoFP-NH2 is the fluorescent form of the lysosomal turn-on fluorescent probe for carbon monoxide (CO) lysoFP-NO2.
In vitro activity: A lysosome-targetable fluorogenic probe, LysoFP-NO2, was designed and synthesized based on a naphthalimide fluorophore that can detect selectively carbon monoxide (CO) in HEPES buffer (pH 7.4, 37 °C) through the transformation of the nitro group into an amino-functionalized system in the presence of CO. LysoFP-NO2 triggered a "turn-on" fluorescence response to CO with a simultaneous increase of fluorescence intensity by more than 75 times. Reference: Anal Chem. 2018 Feb 20;90(4):2933-2938. https://pubmed.ncbi.nlm.nih.gov/29353475/
In vivo activity: TBD

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 20.0 61.47
DMSO 20.0 61.47
DMSO:PBS (pH 7.2) (1:30) 0.0 0.09
Ethanol 1.0 3.07

Preparing Stock Solutions

The following data is based on the product molecular weight 325.37 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Dhara K, Lohar S, Patra A, Roy P, Saha SK, Sadhukhan GC, Chattopadhyay P. A New Lysosome-Targetable Turn-On Fluorogenic Probe for Carbon Monoxide Imaging in Living Cells. Anal Chem. 2018 Feb 20;90(4):2933-2938. doi: 10.1021/acs.analchem.7b05331. Epub 2018 Feb 1. PMID: 29353475.
In vitro protocol: 1. Dhara K, Lohar S, Patra A, Roy P, Saha SK, Sadhukhan GC, Chattopadhyay P. A New Lysosome-Targetable Turn-On Fluorogenic Probe for Carbon Monoxide Imaging in Living Cells. Anal Chem. 2018 Feb 20;90(4):2933-2938. doi: 10.1021/acs.analchem.7b05331. Epub 2018 Feb 1. PMID: 29353475.
In vivo protocol: TBD

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1. Dhara, K., Lohar, S., Patra, A., et al. A new lysosome-targetable turn-on fluorogenic probe for carbon monoxide imaging in living cells. Anal. Chem. 90(4), 2933-2938 (2018).