WARNING: This product is for research use only, not for human or veterinary use.
MedKoo CAT#: 574080
Description: 6-TAMRA is a fluorescent dye that has commonly been used for the covalent labeling of oligonucleotides for DNA analysis. It displays excitation/emission maxima of 543/572 nm, respectively. 6-Carboxytetramethylrhodamine has been used in various DNA-protein binding studies, DNA FRET experiments, and as a standard reporter or quencher dye in RT-PCR.
MedKoo Cat#: 574080
Chemical Formula: C25H22N2O5
Exact Mass: 430.1529
Molecular Weight: 430.46
Elemental Analysis: C, 69.76; H, 5.15; N, 6.51; O, 18.58
Synonym: 6-Carboxytetramethylrhodamine, 6-TAMRA
IUPAC/Chemical Name: 9-(2,5-dicarboxyphenyl)-3,6-bis(dimethylamino)-xanthylium, inner salt
InChi Key: COCMHKNAGZHBDZ-UHFFFAOYSA-N
InChi Code: InChI=1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)20-11-14(24(28)29)5-8-17(20)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
SMILES Code: [O-]C(C(C=CC(C(O)=O)=C1)=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)=O
Appearance: Very Dark Green to Black Solid
Purity: >90% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Acetonitrile (Slightly), DMSO (Slightly), Methanol (Very Slightly, Heated)
Shelf Life: >3 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
The following data is based on the product molecular weight 430.46 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.15 mL||5.76 mL||11.51 mL|
|5 mM||0.23 mL||1.15 mL||2.3 mL|
|10 mM||0.12 mL||0.58 mL||1.15 mL|
|50 mM||0.02 mL||0.12 mL||0.23 mL|
1. Kvach, M.V., Stepanova, I.A., Prokhorenko, I.A., et al. Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes. Bioconjug. Chem. 20(8), 1673-1682 (2009).
2. Han, C., Chen, T., and Zu, L. Terbium chelate as donor label in time-resolved fluorescence energy transfer study of DNA hybridization. Chem. Phys. Lett. 500(4-6), 323-326 (2010).
Emission: 572 nm
Excitation: 543 nm
λmax: 253, 353, 546 nm