4-Thiouridine
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MedKoo CAT#: 574063

CAS#: 13957-31-8

Description: 4-Thiouridine, also known as 4-SU, is a photoactivatable ribonucleoside analog that is widely used for RNA analysis, including short-range RNA-RNA crosslinking and nascent RNA labeling. The crosslinking thio moiety is attached directly to the nucleotide base, thus 4-SU differs from uridine only by a single sulfur substitution. This offers the advantage of incorporating into an RNA chain with minimal structural perturbation and with similar base-pairing properties, reducing the likelihood that substitution will impair RNA interactions or activities.

Chemical Structure

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4-Thiouridine
CAS# 13957-31-8
Instruction

Theoretical Analysis

MedKoo Cat#: 574063
Name: 4-Thiouridine
CAS#: 13957-31-8
Chemical Formula: C9H12N2O5S
Exact Mass: 260.05
Molecular Weight: 260.260
Elemental Analysis: C, 41.53; H, 4.65; N, 10.76; O, 30.74; S, 12.32

Price and Availability

Size Price Availability Quantity
5mg USD 215
25mg USD 335
100mg USD 500
250mg USD 750
500mg USD 1000
1g USD 1250
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Synonym: 4-Thiouridine, 4-SU

IUPAC/Chemical Name: 4-thio-uridine

InChi Key: ZLOIGESWDJYCTF-XVFCMESISA-N

InChi Code: InChI=1S/C9H12N2O5S/c12-3-4-6(13)7(14)8(16-4)11-2-1-5(17)10-9(11)15/h1-2,4,6-8,12-14H,3H2,(H,10,15,17)/t4-,6-,7-,8-/m1/s1

SMILES Code: OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=CC(NC2=O)=S)O1

Appearance: Yellow Solid

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: DMSO (Slightly), Methanol (Slightly)

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 260.26 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1. Burger, K., Mühl, B., Kellner, M., et al. 4-Thiouridine inhibits rRNA synthesis and causes a nucleolar stress response. RNA Biol. 10(10), 1623-1630 (2013).

2. Thomas, G., and Favre, A. 4-Thiouridine triggers both growth delay induced by near-ultraviolet light and photoprotection. European Journal of Biochemistry 113(1), 67-74 (1980).

3. Harris, M.E., and Christian, E.L. RNA crosslinking methods. Methods in Enzymology 468, 127-146 (2009).