Lysopine

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 530785

CAS#: 34522-31-1

Description: Lysopine is a bioactive chemical.

Chemical Structure

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Lysopine
CAS# 34522-31-1
Instruction

Theoretical Analysis

MedKoo Cat#: 530785
Name: Lysopine
CAS#: 34522-31-1
Chemical Formula: C9H18N2O4
Exact Mass: 218.13
Molecular Weight: 218.253
Elemental Analysis: C, 49.53; H, 8.31; N, 12.84; O, 29.32

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

Request quote for custom synthesis

Synonym: Lysopine

IUPAC/Chemical Name: (R)-N(2)-(1-Carboxyethyl)-L-lysine

InChi Key: ZZYYVZYAZCMNPG-RQJHMYQMSA-N

InChi Code: InChI=1S/C9H18N2O4/c1-6(8(12)13)11-7(9(14)15)4-2-3-5-10/h6-7,11H,2-5,10H2,1H3,(H,12,13)(H,14,15)/t6-,7+/m1/s1

SMILES Code: NCCCC[C@@H](C(O)=O)N[C@@H](C(O)=O)C

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 218.25 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Venter L, Jansen van Rensburg PJ, Loots DT, Vosloo A, Lindeque JZ. From untargeted LC-QTOF analysis to characterisation of opines in abalone adductor muscle: Theory meets practice. J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Dec 15;1071:44-48. doi: 10.1016/j.jchromb.2017.01.025. Epub 2017 Feb 24. PubMed PMID: 28262445.

2: Al-Subiai SN, Jha AN, Moody AJ. Contamination of bivalve haemolymph samples by adductor muscle components: implications for biomarker studies. Ecotoxicology. 2009 Apr;18(3):334-42. doi: 10.1007/s10646-008-0287-9. Epub 2008 Dec 14. PubMed PMID: 19083092.

3: Chilton WS, Petit A, Chilton MD, Dessaux Y. Structure and characterization of the crown gall opines heliopine, vitopine and ridéopine. Phytochemistry. 2001 Sep;58(1):137-42. PubMed PMID: 11524123.

4: Coughlan M, O'Carra P. The lysopine and octopine dehydrogenase activities of Mytilus edulis are catalysed by a single enzyme. Biochem Soc Trans. 1996 Feb;24(1):128S. PubMed PMID: 8674614.

5: Dairi T, Asano Y. Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C. Appl Environ Microbiol. 1995 Aug;61(8):3169-71. PubMed PMID: 7487048; PubMed Central PMCID: PMC167592.

6: Bell CR, Moore LW, Canfield ML. Growth of Octopine-Catabolizing Pseudomonas spp. under Octopine Limitation in Chemostats and Their Potential To Compete with Agrobacterium tumefaciens. Appl Environ Microbiol. 1990 Sep;56(9):2834-9. PubMed PMID: 16348292; PubMed Central PMCID: PMC184851.

7: Gleba YY, Kanevsky IF, Skarzhynskaya MV, Komarnitsky IK, Cherep NN. Hybrids between tobacco crown gall cells and normal somatic cells of Atropa belladonna : Isolation and characterization of cell lines. Plant Cell Rep. 1986 Oct;5(5):394-7. doi: 10.1007/BF00268611. PubMed PMID: 24248308.

8: Kanevsky IF, Gleba YY. Analysis of characters coded for by T-DNA using hybridization between tumorous and normal plant cells. Plant Cell Rep. 1986 Oct;5(5):352-5. doi: 10.1007/BF00268600. PubMed PMID: 24248297.

9: Graves AC, Goldman SL. The transformation of Zea mays seedlings with Agrobacterium tumefaciens. Plant Mol Biol. 1986 Jan;7(1):43-50. doi: 10.1007/BF00020130. PubMed PMID: 24302156.

10: Le Goff L, Beljanski M. The in vitro effects of opines and other compounds on DNAs originating from bacteria, and from healthy and tumorous plant tissues. Exp Cell Biol. 1985;53(6):335-50. PubMed PMID: 3935498.

11: Dahl GA, Tempé J. Studies on the use of toxic precursor analogs of opines to select transformed plant cells. Theor Appl Genet. 1983 Sep;66(3-4):233-9. doi: 10.1007/BF00251151. PubMed PMID: 24263922.

12: Schröder J, Hillebrand A, Klipp W, Pühler A. Expression of plant tumor-specific proteins in minicells of Escherichia coli: a fusion protein of lysopine dehydrogenase with chloramphenicol acetyltransferase. Nucleic Acids Res. 1981 Oct 24;9(20):5187-202. PubMed PMID: 6117827; PubMed Central PMCID: PMC327514.

13: Wullems GJ, Molendijk L, Ooms G, Schilperoort RA. Differential expression of crown gall tumor markers in transformants obtained after in vitro Agrobacterium tumefaciens-induced transformation of cell wall regenerating protoplasts derived from Nicotiana tabacum. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4344-8. PubMed PMID: 16593059; PubMed Central PMCID: PMC319786.

14: Otten L, De Greve H, Hernalsteens JP, Van Montagu M, Schieder O, Straub J, Schell J. Mendelian transmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens. Mol Gen Genet. 1981;183(2):209-13. PubMed PMID: 6948997.

15: Otten LA, Schilperoort RA. A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities. Biochim Biophys Acta. 1978 Dec 8;527(2):497-500. PubMed PMID: 31918.

16: Kemp JD. In Vivo Synthesis of Crown Gall-specific Agrobacterium tumefaciens-directed Derivatives of Basic Amino Acids. Plant Physiol. 1978 Jul;62(1):26-30. PubMed PMID: 16660462; PubMed Central PMCID: PMC1092048.

17: Otten LA, Vreugdenhil D, Schilperoort RA. Properties of D(+)-lysopine dehydrogenase from crown gall tumour tissue. Biochim Biophys Acta. 1977 Dec 8;485(2):268-77. PubMed PMID: 21695.

18: Hack E, Kemp JD. Comparison of octopine, histopine, lysopine, and octopinic acid synthesizing activities in sunflower crown gall tissues. Biochem Biophys Res Commun. 1977 Sep 23;78(2):785-91. PubMed PMID: 20891.

19: Klapwijk PM, Hooykaas PJ, Kester HC, Schilperoort RA, RORSCH A. Isolation and characterization of Agrobacterium tumefaciens mutants affected in the utilization of octopine, octopinic acid and lysopine. J Gen Microbiol. 1976 Sep;96(1):155-63. PubMed PMID: 978177.

20: Lippincott BB, Lippincott JA. Induction of responsiveness to octopine and lysopine in crown-gall tumors. Plant Physiol. 1975 Aug;56(2):213-5. PubMed PMID: 16659274; PubMed Central PMCID: PMC541791.