Saikosaponin C
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MedKoo CAT#: 461706

CAS#: 20736-08-7

Description: Saikosaponin C is one of the major active constituents of dried Radix bupleuri root that has been widely used in China to treat a variety of conditions. Recently, saikosaponins have been reported to have properties of cell growth inhibition, inducing cancer cells differentiation and apoptosis.


Chemical Structure

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Saikosaponin C
CAS# 20736-08-7

Theoretical Analysis

MedKoo Cat#: 461706
Name: Saikosaponin C
CAS#: 20736-08-7
Chemical Formula: C48H78O17
Exact Mass: 926.52
Molecular Weight: 927.130
Elemental Analysis: C, 62.18; H, 8.48; O, 29.34

Price and Availability

Size Price Availability Quantity
1mg USD 350 2 weeks
5mg USD 530 2 Weeks
10mg USD 800 2 Weeks
20mg USD 1050 2 Weeks
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Synonym: Saikosaponin C;

IUPAC/Chemical Name: (2S,3R,4R,5R,6S)-2-(((2R,3R,4S,5S,6R)-4,5-dihydroxy-2-((6aR,6bS,8R,12aS,14bS)-8-hydroxy-4,4,6a,6b,11,11,14b-heptamethyl-2,3,4,4a,5,6,6a,6b,7,8,10,11,12,12a,14a,14b-hexadecahydro-1H,9H-12b,8a-(epoxymethano)picen-3-yl)-6-(hydroxymethyl)-2-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)tetrahydro-2H-pyran-3-yl)oxy)-6-methyltetrahydro-2H-pyran-3,4,5-triol

InChi Key: IUZOALRTJNKYRZ-ANYCVEQWSA-N

InChi Code: InChI=1S/C48H78O17/c1-22-30(52)33(55)36(58)39(61-22)63-38-35(57)32(54)24(20-50)64-48(38,65-40-37(59)34(56)31(53)23(19-49)62-40)26-9-12-43(6)25(42(26,4)5)10-13-44(7)27(43)11-14-47-28-17-41(2,3)15-16-46(28,21-60-47)29(51)18-45(44,47)8/h11,14,22-40,49-59H,9-10,12-13,15-21H2,1-8H3/t22-,23+,24+,25?,26?,27?,28-,29+,30-,31+,32+,33+,34-,35-,36+,37+,38+,39-,40-,43-,44+,45-,46?,47?,48+/m0/s1

SMILES Code: C[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](O[C@@H]2[C@@H](O)[C@H](O)[C@@H](CO)O[C@@]2(O[C@H]3[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O3)C4CC[C@@]5(C)C(CC[C@]6(C)C5C=CC78[C@@]6(C)C[C@@H](O)C9(CO8)[C@@H]7CC(C)(C)CC9)C4(C)C)O1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

More Info:

Product Data:
Biological target: Saikosaponin C targets amyloid beta and tau in Alzheimer's disease. Saikosaponin C inhibits the secretion of both Aβ1-40 and Aβ1-42, and suppresses abnormal tau phosphorylation, but shows no effect on BACE1 activity and expression.
In vitro activity: Saikosaponin C may offer a promising therapy for Alzheimer's disease and other neurodegenerative diseases. It reduces Aβ peptide release, does not impact BACE1, inhibits abnormal tau phosphorylation, promotes neurite growth and microtubules, and enhances synaptic markers like synaptophysin and PSD-95. Saikosaponin C has potential as a multifaceted therapeutic agent. Reference: J Neurochem. 2016 Mar;136(6):1232-1245. https://pubmed.ncbi.nlm.nih.gov/26710244/
In vivo activity: Saikosaponin C attenuated chronic social defeat stress-induced depression-like behavior in a murine model. Saikosaponin C alleviated depressive-like behavior; inhibited inflammatory cytokine levels; increased dendritic spine density; promoted synaptic plasticity; downregulated IL-6, STAT3 and DNMT1 expression; decreased the percentage of monocytes in peripheral blood; and suppressed neuroinflammation. Reference: Int Immunopharmacol. 2023 May;118:110043. https://pubmed.ncbi.nlm.nih.gov/36965369/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 100.0 107.86

Preparing Stock Solutions

The following data is based on the product molecular weight 927.13 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Pan Y, Ke Z, Ye H, Sun L, Ding X, Shen Y, Zhang R, Yuan J. Saikosaponin C exerts anti-HBV effects by attenuating HNF1α and HNF4α expression to suppress HBV pgRNA synthesis. Inflamm Res. 2019 Dec;68(12):1025-1034. doi: 10.1007/s00011-019-01284-2. Epub 2019 Sep 17. PMID: 31531682; PMCID: PMC7079752. 2. Lee TH, Park S, You MH, Lim JH, Min SH, Kim BM. A potential therapeutic effect of saikosaponin C as a novel dual-target anti-Alzheimer agent. J Neurochem. 2016 Mar;136(6):1232-1245. doi: 10.1111/jnc.13515. Epub 2016 Jan 21. PMID: 26710244. 3. Bai Z, Gao T, Zhang R, Lu Y, Tian J, Wang T, Zhao K, Wang H. Inhibition of IL-6 methylation by Saikosaponin C regulates neuroinflammation to alleviate depression. Int Immunopharmacol. 2023 May;118:110043. doi: 10.1016/j.intimp.2023.110043. Epub 2023 Mar 23. PMID: 36965369.
In vitro protocol: 1. Pan Y, Ke Z, Ye H, Sun L, Ding X, Shen Y, Zhang R, Yuan J. Saikosaponin C exerts anti-HBV effects by attenuating HNF1α and HNF4α expression to suppress HBV pgRNA synthesis. Inflamm Res. 2019 Dec;68(12):1025-1034. doi: 10.1007/s00011-019-01284-2. Epub 2019 Sep 17. PMID: 31531682; PMCID: PMC7079752. 2. Lee TH, Park S, You MH, Lim JH, Min SH, Kim BM. A potential therapeutic effect of saikosaponin C as a novel dual-target anti-Alzheimer agent. J Neurochem. 2016 Mar;136(6):1232-1245. doi: 10.1111/jnc.13515. Epub 2016 Jan 21. PMID: 26710244.
In vivo protocol: 1. Bai Z, Gao T, Zhang R, Lu Y, Tian J, Wang T, Zhao K, Wang H. Inhibition of IL-6 methylation by Saikosaponin C regulates neuroinflammation to alleviate depression. Int Immunopharmacol. 2023 May;118:110043. doi: 10.1016/j.intimp.2023.110043. Epub 2023 Mar 23. PMID: 36965369.

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1: Chen YC, Wang HM, Niu QX, Ye DY, Liang GW. Binding between Saikosaponin C and Human Serum Albumin by Fluorescence Spectroscopy and Molecular Docking. Molecules. 2016 Jan 28;21(2):153. doi: 10.3390/molecules21020153. PubMed PMID: 26828474.

2: Hu SC, Lee IT, Yen MH, Lin CC, Lee CW, Yen FL. Anti-melanoma activity of Bupleurum chinense, Bupleurum kaoi and nanoparticle formulation of their major bioactive compound saikosaponin-d. J Ethnopharmacol. 2016 Feb 17;179:432-42. doi: 10.1016/j.jep.2015.12.058. Epub 2015 Dec 31. PubMed PMID: 26748071.

3: Lee TH, Park S, You MH, Lim JH, Min SH, Kim BM. A potential therapeutic effect of saikosaponin C as a novel dual-target anti-Alzheimer agent. J Neurochem. 2016 Mar;136(6):1232-1245. doi: 10.1111/jnc.13515. Epub 2016 Jan 21. PubMed PMID: 26710244.

4: Zhang Y, Liu F, Zhang X, Xu T, Quan W, Wang H, Shi J, Dai Z, Wu B, Wu Q. Recognition and identification of active components from Radix Bupleuri using human neuroblastoma SH-SY5Y cells. Biomed Chromatogr. 2016 Mar;30(3):440-6. doi: 10.1002/bmc.3567. Epub 2015 Aug 27. PubMed PMID: 26194341.

5: Lee TH, Chang J, Kim BM. Saikosaponin C inhibits lipopolysaccharide-induced apoptosis by suppressing caspase-3 activation and subsequent degradation of focal adhesion kinase in human umbilical vein endothelial cells. Biochem Biophys Res Commun. 2014 Mar 14;445(3):615-21. doi: 10.1016/j.bbrc.2014.02.046. Epub 2014 Feb 22. PubMed PMID: 24565837.

6: Yu KU, Jang IS, Kang KH, Sung CK, Kim DH. Metabolism of saikosaponin c and naringin by human intestinal bacteria. Arch Pharm Res. 1997 Oct;20(5):420-4. doi: 10.1007/BF02973933. PubMed PMID: 18982483.

7: Shyu KG, Tsai SC, Wang BW, Liu YC, Lee CC. Saikosaponin C induces endothelial cells growth, migration and capillary tube formation. Life Sci. 2004 Dec 31;76(7):813-26. PubMed PMID: 15581913.