WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 563561

CAS#: 3348-03-6

Description: 6-CFDA is a cell membranes permeatable non-fluorescent prodrug of 5-carboxyfluorescein. It shows strongly fluorescent to differentiate viable cells from apoptotic cells after being enzymatically hydrolyzed inside cells.

Chemical Structure

CAS# 3348-03-6

Theoretical Analysis

MedKoo Cat#: 563561
Name: 6-CFDA
CAS#: 3348-03-6
Chemical Formula: C25H16O9
Exact Mass: 460.0794
Molecular Weight: 460.39
Elemental Analysis: C, 65.22; H, 3.50; O, 31.28

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: 6-CFDA; 6 CFDA; 6CFDA; CFDA-6; CFDA 6; CFDA6;

IUPAC/Chemical Name: 3',6'-Diacetyloxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid


InChi Code: InChI=1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)

SMILES Code: O=C(C1=CC=C2C(OC3(C4=C(OC5=C3C=CC(OC(C)=O)=C5)C=C(OC(C)=O)C=C4)C2=C1)=O)O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

Preparing Stock Solutions

The following data is based on the product molecular weight 460.39 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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