AK-295

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 592344

CAS#: 160399-35-9

Description: AK 295, also known asCX 295, is a dipeptide alpha-ketoamide calpain inhibitor.

Chemical Structure

img
AK-295
CAS# 160399-35-9
Instruction

Theoretical Analysis

MedKoo Cat#: 592344
Name: AK-295
CAS#: 160399-35-9
Chemical Formula: C26H40N4O6
Exact Mass: 504.29
Molecular Weight: 504.630
Elemental Analysis: C, 61.88; H, 7.99; N, 11.10; O, 19.02

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

Request quote for custom synthesis

Synonym: AK 295; AK-295; AK295; CX295; CX-295; CX 295;

IUPAC/Chemical Name: Carbamic acid, ((1S)-1-(((1-ethyl-3-((3-(4-morpholinyl)propyl)amino)-2,3-dioxopropyl)amino)carbonyl)-3-methylbutyl)-, phenylmethyl ester

InChi Key: TZVQRMYLYQNBOA-KEKNWZKVSA-N

InChi Code: InChI=1S/C26H40N4O6/c1-4-21(23(31)25(33)27-11-8-12-30-13-15-35-16-14-30)28-24(32)22(17-19(2)3)29-26(34)36-18-20-9-6-5-7-10-20/h5-7,9-10,19,21-22H,4,8,11-18H2,1-3H3,(H,27,33)(H,28,32)(H,29,34)/t21?,22-/m0/s1

SMILES Code: O=C(OCC1=CC=CC=C1)N[C@H](C(NC(CC)C(C(NCCCN2CCOCC2)=O)=O)=O)CC(C)C

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 504.63 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Colak A, Kaya M, Karaoğlan A, Sağmanligil A, Akdemir O, Sahan E, Celik O. Calpain inhibitor AK 295 inhibits calpain-induced apoptosis and improves neurologic function after traumatic spinal cord injury in rats. Neurocirugia (Astur). 2009 Jun;20(3):245-54. PubMed PMID: 19575128.

2: Wang MS, Davis AA, Culver DG, Wang Q, Powers JC, Glass JD. Calpain inhibition protects against Taxol-induced sensory neuropathy. Brain. 2004 Mar;127(Pt 3):671-9. Epub 2004 Feb 4. PubMed PMID: 14761904.

3: Caba E, Brown QB, Kawasaki B, Bahr BA. Peptidyl alpha-keto amide inhibitor of calpain blocks excitotoxic damage without affecting signal transduction events. J Neurosci Res. 2002 Mar 15;67(6):787-94. PubMed PMID: 11891793.

4: Blomgren K, Zhu C, Wang X, Karlsson JO, Leverin AL, Bahr BA, Mallard C, Hagberg H. Synergistic activation of caspase-3 by m-calpain after neonatal hypoxia-ischemia: a mechanism of "pathological apoptosis"? J Biol Chem. 2001 Mar 30;276(13):10191-8. Epub 2000 Dec 21. PubMed PMID: 11124942.

5: DeBiasi RL, Edelstein CL, Sherry B, Tyler KL. Calpain inhibition protects against virus-induced apoptotic myocardial injury. J Virol. 2001 Jan;75(1):351-61. PubMed PMID: 11119604; PubMed Central PMCID: PMC113928.

6: Wang MS, Wu Y, Culver DG, Glass JD. Pathogenesis of axonal degeneration: parallels between Wallerian degeneration and vincristine neuropathy. J Neuropathol Exp Neurol. 2000 Jul;59(7):599-606. PubMed PMID: 10901231.

7: Saatman KE, Zhang C, Bartus RT, McIntosh TK. Behavioral efficacy of posttraumatic calpain inhibition is not accompanied by reduced spectrin proteolysis, cortical lesion, or apoptosis. J Cereb Blood Flow Metab. 2000 Jan;20(1):66-73. PubMed PMID: 10616794.

8: Blomgren K, Hallin U, Andersson AL, Puka-Sundvall M, Bahr BA, McRae A, Saido TC, Kawashima S, Hagberg H. Calpastatin is up-regulated in response to hypoxia and is a suicide substrate to calpain after neonatal cerebral hypoxia-ischemia. J Biol Chem. 1999 May 14;274(20):14046-52. PubMed PMID: 10318818.

9: Bartus RT, Chen EY, Lynch G, Kordower JH. Cortical ablation induces a spreading calcium-dependent, secondary pathogenesis which can be reduced by inhibiting calpain. Exp Neurol. 1999 Feb;155(2):315-26. PubMed PMID: 10072307.

10: James T, Matzelle D, Bartus R, Hogan EL, Banik NL. New inhibitors of calpain prevent degradation of cytoskeletal and myelin proteins in spinal cord in vitro. J Neurosci Res. 1998 Jan 15;51(2):218-22. PubMed PMID: 9469575.

11: Saatman KE, Murai H, Bartus RT, Smith DH, Hayward NJ, Perri BR, McIntosh TK. Calpain inhibitor AK295 attenuates motor and cognitive deficits following experimental brain injury in the rat. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3428-33. PubMed PMID: 8622952; PubMed Central PMCID: PMC39625.

12: Bartus RT, Hayward NJ, Elliott PJ, Sawyer SD, Baker KL, Dean RL, Akiyama A, Straub JA, Harbeson SL, Li Z, et al. Calpain inhibitor AK295 protects neurons from focal brain ischemia. Effects of postocclusion intra-arterial administration. Stroke. 1994 Nov;25(11):2265-70. PubMed PMID: 7974554.