N2-OH-PhIP
featured

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 563231

CAS#: 124489-20-9

Description: N2-OH-PhIP is a PhIP metabolite.


Chemical Structure

img
N2-OH-PhIP
CAS# 124489-20-9

Theoretical Analysis

MedKoo Cat#: 563231
Name: N2-OH-PhIP
CAS#: 124489-20-9
Chemical Formula: C13H12N4O
Exact Mass: 240.10
Molecular Weight: 240.260
Elemental Analysis: C, 64.99; H, 5.03; N, 23.32; O, 6.66

Price and Availability

Size Price Availability Quantity
5mg USD 305 2 Weeks
10mg USD 500 2 Weeks
25mg USD 895 2 Weeks
50mg USD 1600 2 Weeks
100mg USD 2250 2 Weeks
Bulk inquiry

Synonym: N2-OH-PhIP; N2 OH PhIP; N2OHPhIP; N-Hydroxy PhIP; N-OH PhIP;

IUPAC/Chemical Name: 2-Hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine

InChi Key: DCVWQAGUQFBCTF-UHFFFAOYSA-N

InChi Code: InChI=1S/C13H12N4O/c1-17-11-7-10(9-5-3-2-4-6-9)8-14-12(11)15-13(17)16-18/h2-8,18H,1H3,(H,14,15,16)

SMILES Code: CN1C(NO)=NC2=NC=C(C3=CC=CC=C3)C=C21

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 240.26 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Vlaming ML, Teunissen SF, van de Steeg E, van Esch A, Wagenaar E, Brunsveld L, de Greef TF, Rosing H, Schellens JH, Beijnen JH, Schinkel AH. Bcrp1;Mdr1a/b;Mrp2 combination knockout mice: altered disposition of the dietary carcinogen PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) and its genotoxic metabolites. Mol Pharmacol. 2014 Mar;85(3):520-30. doi: 10.1124/mol.113.088823. Epub 2013 Dec 13. PubMed PMID: 24334255.

2: Chen C, Ma X, Malfatti MA, Krausz KW, Kimura S, Felton JS, Idle JR, Gonzalez FJ. A comprehensive investigation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) metabolism in the mouse using a multivariate data analysis approach. Chem Res Toxicol. 2007 Mar;20(3):531-42. Epub 2007 Feb 6. PubMed PMID: 17279779; PubMed Central PMCID: PMC1850849.

3: Kulp KS, Knize MG, Fowler ND, Salmon CP, Felton JS. PhIP metabolites in human urine after consumption of well-cooked chicken. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Mar 25;802(1):143-53. PubMed PMID: 15036006.

4: Prabhu S, Lee MJ, Hu WY, Winnik B, Yang I, Buckley B, Hong JY. Determination of 2-amino-1-methyl-6-phenylimidazo[4,5- b]pyridine (PhIP) and its metabolite 2-hydroxyamino-PhIP by liquid chromatography/electrospray ionization-ion trap mass spectrometry. Anal Biochem. 2001 Nov 15;298(2):306-13. PubMed PMID: 11700987.

5: Crofts FG, Sutter TR, Strickland PT. Metabolism of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine by human cytochrome P4501A1, P4501A2 and P4501B1. Carcinogenesis. 1998 Nov;19(11):1969-73. PubMed PMID: 9855011.

6: Crofts FG, Strickland PT, Hayes CL, Sutter TR. Metabolism of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) by human cytochrome P4501B1. Carcinogenesis. 1997 Sep;18(9):1793-8. PubMed PMID: 9328177.

7: Dragsted LO, Alexander J, Wallin H, Frandsen H, Vang O. Bioactivation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]-pyridine by liver microsomes from three different rat strains. Pharmacol Toxicol. 1993 Jun;72(6):388-93. PubMed PMID: 8361949.