LM22B-10
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 555271

CAS#: 342777-54-2

Description: LM22B-10 is a TrkB and TrkC agonist. LM22B-10 exhibits neurotrophic activity (EC50 = 200-300 nM). LM22B-10 improves survival and increases neurite outgrowth in hippocampal cells in vitro. LM22B-10 increased cell survival and strongly accelerated neurite outgrowth, superseding the effects of brain-derived neurotrophic factor (BDNF), NT-3 or the two combined.

Chemical Structure

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LM22B-10
CAS# 342777-54-2
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 555271
Name: LM22B-10
CAS#: 342777-54-2
Chemical Formula: C27H33ClN2O4
Exact Mass: 484.21
Molecular Weight: 485.021
Elemental Analysis: C, 66.86; H, 6.86; Cl, 7.31; N, 5.78; O, 13.19

Price and Availability

Size Price Availability Quantity
50mg USD 450
100mg USD 850
200mg USD 1450
500mg USD 2250
1g USD 3450
2g USD 5650
5g USD 7850
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Synonym: LM22B-10; LM22B 10; LM22B10; LM 22B10; LM-22B10; LM22B10;

IUPAC/Chemical Name: 2,2',2',2'''-[[(4-Chlorophenyl)methylene]bis(4,1-phenylenenitrilo)]tetrakisethanol

InChi Key: QCXQLSGBOUUVNH-UHFFFAOYSA-N

InChi Code: InChI=1S/C27H33ClN2O4/c28-24-7-1-21(2-8-24)27(22-3-9-25(10-4-22)29(13-17-31)14-18-32)23-5-11-26(12-6-23)30(15-19-33)16-20-34/h1-12,27,31-34H,13-20H2

SMILES Code: ClC1=CC=C(C(C2=CC=C(N(CCO)CCO)C=C2)C3=CC=C(N(CCO)CCO)C=C3)C=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Instruction:
View handling instruction
Biological target: LM22B-10 is an activator of TrkB/TrkC neurotrophin receptor, and can induce TrkB, TrkC, AKT and ERK activation in vitro and in vivo.
In vitro activity: The cells were treated with LM22B-10 (an ERK activator) or transfected with the constitutive activation of the mitogen-activated protein kinase 1 (CaMEK) gene. Administering LM22B-10 or transfecting the CaMEK gene significantly activated the phosphorylation levels of ERK1/2 protein and reduced the proportion of cardiomyocyte apoptosis (P<0.05). Reference: Ann Transl Med. 2022 Nov;10(22):1238. https://pubmed.ncbi.nlm.nih.gov/36544682/
In vivo activity: In vivo model of PNI (perineural invasion) was established via inoculating PANC-1 cells into mice. PANC-1 cells and mice were also treated with LM22B-10 (an activator of TrkC) to confirm the mechanisms involving NT-3/TrkC in PNI of PC both in vivo and in vitro. LM22B-10 partially abolished the effects of MMP1 knockdown both in vivo and in vitro. Reference: J Clin Lab Anal. 2022 Nov;36(11):e24719. https://pubmed.ncbi.nlm.nih.gov/36181286/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 81.4 167.78
Ethanol 44.0 90.61

Preparing Stock Solutions

The following data is based on the product molecular weight 485.02 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Yu F, Liu F, Luo JY, Zhao Q, Wang HL, Fang BB, Li XM, Yang YN. Targeted activation of ERK1/2 reduces ischemia and reperfusion injury in hyperglycemic myocardium by improving mitochondrial function. Ann Transl Med. 2022 Nov;10(22):1238. doi: 10.21037/atm-22-5149. PMID: 36544682; PMCID: PMC9761117. 2. Yang Y, Wu Y, Hou L, Ge X, Song G, Jin H. Heat shock protein 20 suppresses breast carcinogenesis by inhibiting the MAPK and AKT signaling pathways. Oncol Lett. 2022 Nov 3;24(6):462. doi: 10.3892/ol.2022.13582. PMID: 36380873; PMCID: PMC9650602. 3. Xu X, Lu X, Chen L, Peng K, Ji F. Downregulation of MMP1 functions in preventing perineural invasion of pancreatic cancer through blocking the NT-3/TrkC signaling pathway. J Clin Lab Anal. 2022 Nov;36(11):e24719. doi: 10.1002/jcla.24719. Epub 2022 Sep 30. PMID: 36181286; PMCID: PMC9701873. 4. Wang D, Li Y, Dai L, Wang Y, Zhao C, Wang W, Zhang Y, Zhao Y, Yu T. 1,2,3,4,6‑penta‑O‑galloyl‑β‑D‑glucose alleviates inflammation and oxidative stress in diabetic nephropathy rats through MAPK/NF‑κB and ERK/Nrf2/HO‑1 signaling pathways. Exp Ther Med. 2022 Aug 26;24(4):639. doi: 10.3892/etm.2022.11576. PMID: 36160883; PMCID: PMC9468796.
In vitro protocol: 1. Yu F, Liu F, Luo JY, Zhao Q, Wang HL, Fang BB, Li XM, Yang YN. Targeted activation of ERK1/2 reduces ischemia and reperfusion injury in hyperglycemic myocardium by improving mitochondrial function. Ann Transl Med. 2022 Nov;10(22):1238. doi: 10.21037/atm-22-5149. PMID: 36544682; PMCID: PMC9761117. 2. Yang Y, Wu Y, Hou L, Ge X, Song G, Jin H. Heat shock protein 20 suppresses breast carcinogenesis by inhibiting the MAPK and AKT signaling pathways. Oncol Lett. 2022 Nov 3;24(6):462. doi: 10.3892/ol.2022.13582. PMID: 36380873; PMCID: PMC9650602.
In vivo protocol: 1. Xu X, Lu X, Chen L, Peng K, Ji F. Downregulation of MMP1 functions in preventing perineural invasion of pancreatic cancer through blocking the NT-3/TrkC signaling pathway. J Clin Lab Anal. 2022 Nov;36(11):e24719. doi: 10.1002/jcla.24719. Epub 2022 Sep 30. PMID: 36181286; PMCID: PMC9701873. 2. Wang D, Li Y, Dai L, Wang Y, Zhao C, Wang W, Zhang Y, Zhao Y, Yu T. 1,2,3,4,6‑penta‑O‑galloyl‑β‑D‑glucose alleviates inflammation and oxidative stress in diabetic nephropathy rats through MAPK/NF‑κB and ERK/Nrf2/HO‑1 signaling pathways. Exp Ther Med. 2022 Aug 26;24(4):639. doi: 10.3892/etm.2022.11576. PMID: 36160883; PMCID: PMC9468796.

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1: Zhang T, Liao JY, Yu L, Liu GS. Regulating effect of glycyrrhetinic acid on
bronchial asthma smooth muscle proliferation and apoptosis as well as
inflammatory factor expression through ERK1/2 signaling pathway. Asian Pac J Trop
Med. 2017 Dec;10(12):1172-1176. doi: 10.1016/j.apjtm.2017.10.025. Epub 2017 Oct
28. PubMed PMID: 29268974.


2: Yang T, Massa SM, Tran KC, Simmons DA, Rajadas J, Zeng AY, Jang T, Carsanaro
S, Longo FM. A small molecule TrkB/TrkC neurotrophin receptor co-activator with
distinctive effects on neuronal survival and process outgrowth.
Neuropharmacology. 2016 Nov;110(Pt A):343-361. doi:
10.1016/j.neuropharm.2016.06.015. Epub 2016 Jun 19. PubMed PMID: 27334657.